Proportionally controlled dual-chiral covalent organic frameworks via thiol-ene click reaction for efficient enantioseparation.

Developing a universally applicable and commercially hopeful chiral material is a significant challenge for the separation and analysis field. In this study, innovative dual-chiral CCOFs ([SH-β-CD-NALC]-COFs) modified with 6-deoxy-6-mercapto-β-cyclodextrin (SH-β-CD) and N-acetyl-l-cysteine (NALC) are presented. These CCOFs were synthesized using a single, one-step bottom-up approach at room temperature, specifically designed for enantiomer recognition and separation.

Enhanced Two-Step LAMP-CRISPR Assay with an Engineered Polymerase for Contamination-Free and Ultrasensitive DNA Detection.

Current loop-mediated isothermal amplification (LAMP)-coupled clustered regularly interspaced short palindromic repeats (LAMP-CRISPR) biosensing in two-step or one-step formats has been applied to next-generation accurate molecular diagnosis. However, two-step LAMP-CRISPR assays intrinsically confront aerosol contamination, while one-step assays possess a compromised detection performance. To this end, we propose an enhanced two-step LAMP-CRISPR assay (ETL-CRISPR) with an engineered polymerase to mediate ultrasensitive DNA detection and thoroughly eliminate aerosol contamination.

An Antibacterial, Highly Sensitive Strain Sensor Based on an Anionic Copolymer Interpenetrating with κ-Carrageenan.

Polysaccharide-based hydrogels are suitable for use in the field of flexible bioelectronics due to their benign mechanical properties and biocompatibility. However, the preparation of hydrogel sensors with high performance without affecting their physicochemical properties (e.g.

Clinical efficacy of bronchoalveolar lavage in the treatment of small airway diseases in children.

Objective: This study aimed to evaluate the efficacy of bronchoalveolar lavage (BAL) in the treatment of children with small airway diseases.

Methods: Children [ = 112; boys: 76, girls: 36 (ratio 2.1:1); age range: 1 month-10 years; median age: 12 months] with small airway diseases diagnosed by high-resolution computed tomography (HRCT) were enrolled in this study.

Specific serum autoantibodies predict the development and progression of Alzheimer's disease with high accuracy.

Autoimmunity plays a key role in the pathogenesis of Alzheimer's disease (AD). However, whether autoantibodies in peripheral blood can be used as biomarkers for AD has been elusive. Serum samples were obtained from 1,686 participants, including 767 with AD, 146 with mild cognitive impairment (MCI), 255 with other neurodegenerative diseases, and 518 healthy controls.

Sulfonated Hydrogel Formed via CO-in-Water Emulsion: Potential in Antibiotic Removal.

Herein, a green, carbon dioxide-in-water high-internal-phase emulsion (C/W HIPEs) was developed and stabilized with polyvinyl alcohol (PVA) for the formation of chitosan oligosaccharide/poly(acrylamide-co-sodium 4-styrene sulfonate) [COS/P(AM-co-SSS)] monolithic porous hydrogel. The obtained monolith was characterized via FT-IR and SEM. The SEM patterns depicted that the monoliths were interconnected, the void sizes were 78.

Heparin-Mimetic Chitooligosaccharides-Based Monoliths Obtained from C/W Emulsions: Hemocompatibility and Toxin Removal Ability.

Hemoperfusion (HP) is one of the most prominent therapies for treating uremia, hyperbilirubinemia, and acute drug toxicity. The comprehensive performance of currently used porous HP adsorbents needs to be improved due to the impediment to their synthesis strategy. Herein, green carbon dioxide-in-water high internal phase emulsions (C/W HIPEs) were utilized and emulsified with poly(vinyl alcohol) (PVA) for the formation of a heparin-mimetic chitosan oligosaccharides/poly(acrylamide--sodium 4-styrenesulfonate) [COS/P(AM-co-SSS)] monolith, which exhibited good mechanical properties, stable swelling performance, hydrophilic properties, anticoagulant effect, and low hemolysis.

Preparation of Reusable Porous Carbon Nanofibers from Oxidized Coal Liquefaction Residue for Efficient Adsorption in Water Treatment.

Porous carbon nanofibers are commonly used for adsorption processes owing to their high specific surface area and rich pore structure. However, the poor mechanical properties of polyacrylonitrile (PAN)-based porous carbon nanofibers have limited their applications. Herein, we introduced solid waste-derived oxidized coal liquefaction residue (OCLR) into PAN-based nanofibers to obtain activated reinforced porous carbon nanofibers (ARCNF) with enhanced mechanical properties and regeneration for efficient adsorption of organic dyes in wastewater.

Chirality-Controlled Mercapto-β-cyclodextrin Covalent Organic Frameworks for Selective Adsorption and Chromatographic Enantioseparation.

Chiral covalent organic frameworks (CCOFs) benefit from superior stability, abundant chiral environment, and homogeneous pore configuration. In its constructive tactics, only the post-modification method allows for the integration of supramolecular chiral selectors into achiral COFs. Here, the finding utilizes 6-deoxy-6-mercapto-β-cyclodextrin (SH-β-CD) as chiral subunits and 2,5-dihydroxy-1,4-benzenedicarboxaldehyde (DVA) as the platform molecule to synthesize chiral functional monomers through thiol-ene click reactions and directly establish ternary "pendant-type" SH-β-CD COFs.

CUDC-101 as a dual-target inhibitor of EGFR and HDAC enhances the anti-myeloma effects of bortezomib by regulating G2/M cell cycle arrest.

CUDC-101, an effective and multi-target inhibitor of epidermal growth factor receptor (EGFR), histone deacetylase (HDAC), and human epidermal growth factor receptor 2 (HER2), has been reported to inhibit many kinds of cancers, such as acute promyelocytic leukemia and non-Hodgkin's lymphoma. However, no studies have yet investigated whether CUDC-101 is effective against myeloma. Herein, we proved that CUDC-101 effectively inhibits the proliferation of multiple myeloma (MM) cell lines and induces cell apoptosis in a time- and dose-dependent manner.

Quantitative Analysis of Mixed Minerals with Finite Phase Using Thermal Infrared Hyperspectral Technology.

It is crucial but challenging to detect intermediate or end products promptly. Traditional chemical detection methods are time-consuming and cannot detect mineral phase content. Thermal infrared hyperspectral (TIH) technology is an effective means of real-time imaging and can precisely capture the emissivity characteristics of objects.

Calcium silicate/bortezomib combinatory therapy for multiple myeloma.

Multiple myeloma (MM) is the second most common hematologic malignancy. Bortezomib (BOR), a first-generation proteasome inhibitor, is the basic agent for the treatment of MM and has greatly improved the survival of patients with MM. However, the side effects of BOR ( peripheral neuropathy) occur frequently and almost all MM patients eventually develop resistance to BOR and go on to develop refractory relapsed multiple myeloma (RRMM).

Chidamide and venetoclax synergistically exert cytotoxicity on multiple myeloma by upregulating BIM expression.

Background: Multiple myeloma (MM) is the second most common hematologic malignancy with almost all patients eventually having relapse or refractory MM (RRMM), thus novel drugs or combination therapies are needed for improved prognosis. Chidamide and venetoclax, which target histone deacetylase and BCL2, respectively, are two promising agents for the treatment of RRMM.

Results: Herein, we found that chidamide and venetoclax synergistically exert an anti-myeloma effect in vitro in human myeloma cell lines (HMCLs) with a combination index (CI) < 1.

Composite Aerogel Comprised of Sodium Alginate and Bentonite via Supercritical CO Drying: An Efficient Adsorbent for Lysozyme.

To meet the demand for the separation of specific substances, the construction of porous composite aerogels with a high specific surface area and a strong adsorption capacity is still a challenge. Herein, a sodium alginate/bentonite composite aerogel was efficiently prepared through supercritical fluid drying. The aerogel's volume shrank less during supercritical drying, maintaining its original three-dimensional mesh structure.

Enhanced Chiral Recognition Abilities of Cyclodextrin Covalent Organic Frameworks via Chiral/Achiral Functional Modification.

β-Cyclodextrin covalent organic frameworks (β-CD COFs) show great potential in enantioseparation due to their uniformly distributed chiral recognition sites and good chemical stability. The hydroxyl and amino groups of β-CD COFs enable facile post-modification to introduce the desired functionality into the frameworks. In this study, we perform post-modification of β-CD COF with 1,4-butane sultone and [(3,4)-4-acetyloxy-2,5-dioxooxolan-3-yl] acetate to construct two kinds of novel functional β-CD COFs.

Myeloma-derived IL-32γ induced PD-L1 expression in macrophages facilitates immune escape via the PFKFB3-JAK1 axis.

Multiple myeloma (MM) is characterized by an accumulation of monoclonal plasma cells within the bone marrow (BM). Macrophages are an abundant component of myeloma BM microenvironment and support survival of the malignant cells and foster myeloma development and progression by suppression of the immune response. In our previous study, we found that MM patients overexpress pro-inflammatory cytokine interleukin-32 (IL-32).

Bacterial diversity in the intestinal mucosa of heart failure rats treated with Sini Decoction.

Background: Sini Decoction (SND), a classic Chinese medicine prescription, has been proved to have a good effect on heart failure (HF), whereas its underlying mechanism is still unclear. In order to explore the therapeutic mechanism of SND, we combined with 16S rRNA gene sequencing to analyze the composition of gut microflora in rats with HF.

Material And Methods: Twenty Sprague-Dawley (SD) rats were divided into four groups (n = 5): normal group, model group, SND treatment group (SNT group), and metoprolol (Met) treatment group (Meto group).

Zinc Metal-Organic Framework Growing on the Surface of Fruit Peels and Its Photocatalytic Activity.

The development of water treatment materials using environmentally friendly natural biomasses as substitutes plays an increasingly important role in environmental protection. Zeolitic imidazolate framework-8 (ZIF-8) is often used for the catalytic degradation of dye wastewater, but due to its small particle size, its disadvantage of easy agglomeration prevents it from being fully functional. Herein, we report an efficient method for synthesizing biomasses/ZIF-8 using four different fruit peels as carriers.

Posterior subthalamic nucleus (PSTh) mediates innate fear-associated hypothermia in mice.

The neural mechanisms of fear-associated thermoregulation remain unclear. Innate fear odor 2-methyl-2-thiazoline (2MT) elicits rapid hypothermia and elevated tail temperature, indicative of vasodilation-induced heat dissipation, in wild-type mice, but not in mice lacking Trpa1-the chemosensor for 2MT. Here we report that Trpa1 mice show diminished 2MT-evoked c-fos expression in the posterior subthalamic nucleus (PSTh), external lateral parabrachial subnucleus (PBel) and nucleus of the solitary tract (NTS).

Thiazoline-related innate fear stimuli orchestrate hypothermia and anti-hypoxia via sensory TRPA1 activation.

Thiazoline-related innate fear-eliciting compounds (tFOs) orchestrate hypothermia, hypometabolism, and anti-hypoxia, which enable survival in lethal hypoxic conditions. Here, we show that most of these effects are severely attenuated in transient receptor potential ankyrin 1 (Trpa1) knockout mice. TFO-induced hypothermia involves the Trpa1-mediated trigeminal/vagal pathways and non-Trpa1 olfactory pathway.

Discovery of novel VEGFR-2 inhibitors embedding 6,7-dimethoxyquinazoline and diarylamide fragments.

VEGF/VEGFR-2 signaling plays a critical part in tumor angiogenesis. Inhibition of this pathway has been considered as a promising approach for cancer treatment. In this work, a series of 6,7-dimethoxy-4-anilinoquinazoline derivatives bearing diarylamide moiety were designed, synthesized and evaluated as potent inhibitors of VEGFR-2 kinase.

Hyper-Activation of mPFC Underlies Specific Traumatic Stress-Induced Sleep-Wake EEG Disturbances.

Sleep disturbances have been recognized as a core symptom of post-traumatic stress disorders (PTSD). However, the neural basis of PTSD-related sleep disturbances remains unclear. It has been challenging to establish the causality link between a specific brain region and traumatic stress-induced sleep abnormalities.

Identification and characterization of a cDNA encoding a gametocyte-specific protein of the avian coccidial parasite Eimeria necatrix.

Gametocyte proteins of Eimeria spp. are essential components of the oocyst wall, and some of these proteins have been analysed to identify targets of transmission-blocking vaccines against avian coccidiosis. In the present study, a cDNA from E.