Molecular Evolution of Infectious Pancreatic Necrosis Virus in China.

Passive virus surveillance was performed in twenty-nine salmon and trout farms from seven provinces and districts in China during the period 2017-2020. A total of 25 infectious pancreatic necrosis virus (IPNV) isolates were obtained, mainly from rainbow trout (). The molecular evolution of these Chinese IPNV isolates and the previously reported Chinese IPNV strains ChRtm213 and WZ2016 was analyzed, based on their VP2 gene coding region sequences (CDS).

Development of a Colloidal Gold Immunochromatographic Strip for the Rapid Detection of Channel Catfish Virus.

Background: Channel catfish virus disease (CCVD) has resulted in great economic losses and has restricted the development of fisheries. There is therefore, a need for rapid and efficient diagnostic methods to control the spread of CCVD.

Objective: A colloidal gold immunochromatographic strip has been developed for the detection of CCVD.

Downregulation of PSCA promotes gastric cancer proliferation and is related to poor prognosis.

Dysregulation of prostate stem cell antigen (PSCA) has been implicated in human cancers. Studies have reported that PSCA expression is generally high in prostate cancer, which correlates with a worse survival. PSCA is also highly expressed in bladder, ovarian, and pancreatic cancers.

A homogenized approach to classify advanced gastric cancer patients with limited and adequate number of pathologically examined lymph nodes.

Background: The prognosis of gastric cancer patients with a limited number of pathologically examined lymph nodes (eLN, < 16) is dismal compared to those with adequately eLN (≥ 16), yet they are still classified within the same subgroups using the American Joint Committee on Cancer (AJCC) staging system. We aimed at formulating an easy-to-adopt and clinically reliable stratification approach to homogenize the classification for these two categories of patients.

Methods: Patients staged according to the 8th AJCC pathological nodal (N) and tumor-node-metastasis (TNM) classification were stratified into a Limited and Adequate eLN cohort based on their number of pathologically examined LNs.

Glasgow Prognostic Score is superior to ECOG PS as a prognostic factor in patients with gastric cancer with peritoneal seeding.

The Glasgow Prognostic Score (GPS) has been shown to be associated with survival rates in patients with advanced cancer. The present study aimed to compare the GPS with the Eastern Cooperative Oncology Group Performance Status (ECOG PS) in patients with gastric cancer with peritoneal seeding. For the investigation, a total of 384 gastric patients with peritoneal metastasis were retrospectively analyzed.

Clinicopathological Characteristics and Prognostic Value of Signet Ring Cells in Gastric Carcinoma: A Meta-Analysis.

Previous studies of the prognostic value of the signet ring cell (SRC) type have yielded inconsistent results. Therefore, the aim of the present meta-analysis is to explore the clinicopathological characteristics and prognostic value of SRCs. Relevant articles that compared SRC and non-SRC type in PubMed and Web of Science were comprehensively searched.

Selective Gastric Cancer Patients with Peritoneal Seeding Benefit from Gastrectomy after Palliative Chemotherapy: A Propensity Score Matching Analysis.

The present study aimed to explore whether gastric cancer patients with peritoneal seeding after receiving palliative chemotherapy could benefit from gastrectomy and to identify patients with peritoneal seeding who should be selected to receive gastrectomy. A total of 201 gastric cancer patients were diagnosed with peritoneal seeding and received palliative chemotherapy. Propensity score matching (PSM) was performed to balance the selection bias.

Endoscopic ultrasonography compared with multidetector computed tomography for the preoperative staging of gastric cancer: a meta-analysis.

Background: The current study sought to perform a meta-analysis to compare the preoperative staging of endoscopic ultrasonography (EUS) and multidetector computed tomography (MDCT) in gastric carcinoma.

Methods: Articles published between January 1, 2000, and April 1, 2016, that compared EUS with MDCT were included, and data were presented as 2 × 2 tables. The sensitivities, specificities and summary receiver operating characteristic (ROC) curves for T and N staging were calculated using a bivariate mixed effects model.

Establishment and characterization of a heart-derived cell line from goldfish (Carassius auratus).

The goldfish Carassius auratus, a freshwater fish in the family Cyprinidae, was one of the earliest fish to be domesticated for ornamental purposes. A cell line was established from goldfish heart (GH) tissue to create a biological monitoring tool for viral diseases. The GH cell line was optimally maintained at 25 °C in M199 medium supplemented with 10-20% fetal bovine serum.

[Isolation, identification and drug sensitivity of Streptococcus iniae from hybrid sturgeons (Huso dauricus female x Acipenser schrencki male)].

Objective: Sturgeons were the important economic species in Beijing. In July 2012, continuous mortality of cultured hybrid sturgeons was occurred on a farm in Huairou.

Methods: We isolated three pathogens from liver, kidney and spleen of the dying sturgeons with clinical symptoms, marked HRS12718L, HRS12718K and HRS12718S respectively.

[Identification and drug sensitivity of a Plesiomonas shigelloides isolated from diseased sturgeons].

Objective: In 2012, the cumulative mortality of farmed sturgeons in Beijing was almost 60% with various symptoms, including the reddening of the anus with yellow exudation, ascities in the peritoneal cavity, petechial haemorrhages in liver and internal muscle wall, and the swollen spleen.

Methods: We isolated the pathogen from the dying sturgeons with significant pathological signs, and then analyzed its morphological, physiological and biochemical characteristics, taxonomic status, and drug sensitivity. Moreover, the pathogenic characteristic of presumptive pathogens was identified by artificial infection.

Detection of grass carp reovirus (GCRV) with monoclonal antibodies.

Grass carp reovirus (GCRV) is a pathogen that causes hemorrhagic disease of grass carp. It is the most serious infectious disease of carp and causes serious losses of fingerlings of grass carp and black carp. In this study, a recombinant VP4, one of the viral core proteins, was constructed with a histidine tag and expressed at a high level in E.

Actinomycetes and earthy-musty odorous compounds in brackish fishponds in Tianjin, China.

The presence of the odorous compounds, 2-methylisoborneol (MIB) and geosmin, as well as causative microorganisms in brackish intensive cultivation fishponds in Tianjin, China that had a severe earthy-musty odor were evaluated. The results revealed that MIB was the primary odorous compound present in the Tianjin fishponds, with a concentration ranging from 0.53-5302.

[Process, mechanism, and research method of fish invasion].

Fish invasion has become a serious environmental and economic damage, and obtained a priority concern by the conservation ichthyologists and land managers. A better understanding of the invasive mechanisms is of significance in developing ecological theories and in controlling exotic pests. In this paper, the progress in studying the mechanisms of fish invasion was reviewed, involving the biological characteristics of exotic fish, their adaptive evolution, interactions among exotic and indigenous fish, and relationships between species diversity and community resistance to invasion, etc.