[Monoclonal antibodies against squamous epithelial carcinomas of the upper aerodigestive tract].

Background: Squamous cell carcinoma is the most common cancer of the head and neck. The application of monoclonal hybridoma technology to the identification and characterization of molecules preferentially expressed in carcinoma cells may provide potentially important diagnostic and therapeutic tools.

Methods: After immunization of mice with viable squamous cell carcinoma cells derived from human tumors of the upper aerodigestive tract hybridomas were selectively grown for production of monoclonal antibodies.

[Nd:YAG laser light-induced reduction of the nasal turbinates].

Background: Different types of laser have been used for reduction of hyperplastic inferior turbinates in the recent years with good results. Reports about the long-term benefit of the laser treatment are rare.

Patients And Methods: At the Dept.

(1,3-Dimethyluracil-5-yl)mercury(II): Preparative, Structural, and NMR Spectroscopic Studies of an Analog of CH(3)Hg(II).

The solution behavior of (1,3-DimeU-C5)Hg(CH(3)COO) (1a) (1,3-DimeU = 1,3-dimethyluracil) with regard to acetate replacement by anions X (Cl(-), Br(-), I(-), NO(3)(-), SCN(-), CN(-)) and by other model nucleobases (1-methylcytosine, 1-MeC, 1-methyluracil, 1-MeUH, 1-methylthymine, 1-MeTH, 9-ethylguanine, 9-EtGH, and 2-thiouracil, 2-ThioUH) has been studied, primarily by means of (1)H and (199)Hg NMR spectroscopy. Moreover, the bis(1,3-DimeU-C5) complex of Hg has been crystallized and studied by X-ray crystallography. 7a: orthorhombic system, space group Fdd2, a = 14.

[Incidence of point mutations in Ki-ras codon 12 and 13 in squamous epithelial carcinomas of the head-neck region].

Background: Point mutations of ras genes are one of the most frequent genetic alterations in human malignancies with up to over 95% in certain tumors. Some studies have presumed a low incidence of point mutations in Ki-ras gene codons 12 and 13 in squamous cell carcinoma of the head and neck (SCCHN). The objective of this study was to gain more information about the significance of point mutations in Ki-ras codons 12 and 13 by investigation of a high number of cases.

[Immune response to p53 protein in patients with squamous epithelial carcinoma of the upper aerodigestive tract is independent of p53 point mutation].

Objective: Mutations of the p53 gene are the most prevalent genomic changes in human malignancies. The detection of these mutations is usually carried out by gene sequencing or with immunohistochemical methods. A new approach is to search for p53 antibodies in serum of cancer patients.

Model of the Second Most Abundant Cisplatin-DNA Cross-Link: X-ray Crystal Structure and Conformational Analysis of cis-[(NH(3))(2)Pt(9-MeA-N7)(9-EtGH-N7)](NO(3)).2H(2)O (9-MeA = 9-Methyladenine; 9-EtGH = 9-Ethylguanine).

A model compound of the second most abundant DNA adduct of the antitumor agent cisplatin has been synthesized and structurally and spectroscopically characterized and its conformational behavior examined: cis-[(NH(3))(2)Pt(9-MeA-N7)(9-EtGH-N7)](NO(3))(2).2H(2)O (9-MeA = 9-methyladenine; 9-EtGH = 9-ethylguanine) crystallizes in the monoclinic system, space group P2(1)/n (No. 14) with a = 7.

Leiomyosarcoma of the nasal cavity. Case report and literature review.

Leiomyosarcoma (LMS) accounts for approximately 7% of all soft tissue sarcomas and occurs most frequently in the gastrointestinal tract and uterus. LMS of the nasal cavity and paranasal sinuses are rare and only about 40 cases of LMS of the nose and paranasal sinuses have been reported in the literature. A further case of LMS of the right turbinate is presented, treated twice by surgery.

The Uracil C(5) Position as a Metal Binding Site: Solution and X-ray Crystal Structure Studies of Pt(II) and Hg(II) Compounds.

1,3-Dimethyluracil (1,3-DimeU) reacts with trans-[(CH(3)NH(2))(2)Pt(H(2)O)(2)](+) to give trans-[(CH(3)NH(2))(2)Pt(1,3-DimeU-C5)(H(2)O)]X (X = NO(3)(-), 1a, ClO(4)(-), 1b) and subsequently with NaCl to give trans-(CH(3)NH(2))(2)Pt(1,3-DimeU-C5)Cl (2) or with NH(3) to yield trans-[(CH(3)NH(2))(2)Pt(1,3-DimeU-C5)(NH(3))]ClO(4) (3). In a similar way, (dien)Pt(II) forms [dienPt(1,3-DimeU-C5)](+) (4). Reactions leading to formation of 1 and 4 are slow, taking days.

Extent of the Acidification by N7-Coordinated cis-Diammine-Platinum(II) on the Acidic Sites of Guanine Derivatives.

Coordination of two monoprotonated 2'-deoxyguanosine 5'-monophosphate species, H(dGMP)(-), via N7 to cis-(NH(2))(2)Pt(2+) gives the complex cis-(NH(2))(2)Pt(H.dGMP)(2) which is a four-protonic acid. The corresponding acidity constants were measured by potentiometric pH titrations (25; I = 0.

Recurrent laryngeal papillomatosis. Retrospective analysis of 95 patients and review of the literature.

Background: Laryngeal papillomatosis is a benign neoplastic disease which is associated with and probably by the Human Papilloma Virus. It can be of significant importance for affected patients because of its recurrent clinical course. A great variety of therapeutic measures have been described including surgical removal either with conventional instruments or by using the laser.

[Antibody formation to cellular p53 protein in patients with squamous cell carcinomas of the upper respiratory and digestive system].

Background: The mutated form of the nuclear tumor suppressor protein p53 plays an important role as oncogene in the malignant transformation of cells. A new serologic parameter are antibodies against the p53 protein, that are found in many carcinoma entities and localizations and may have a prognostic relevance. Antibodies against p53 in the serum of patients with squamous cell carcinoma of the head and neck (SCCHN) have not yet been described.

Trans-diammineplatinum(II): what makes it different from cis-DDP? Coordination chemistry of a neglected relative of cisplatin and its interaction with nucleic acids.

The question raised, i.e., "trans-diammineplatinum(II)--what makes it different from its cis isomer?" permits a number of answers, relating to various aspects of the chemistry of the two compounds, but the crucial one concerning antitumor activity is yet to be answered.

[Animal experiment studies of laser effects on lymphatic vessels. A contribution to the discussion of laser surgery segmental resection of carcinomas].

Background: The potential effect of laser surgery on the process of lymphogenic metastases is subject to controversy. In this context it would be of interest to identify a morphological basis for an increased intraoperative entry of tumor cells into the lymphatic system during resection of advanced carcinomas in two or more pieces via laser surgery.

Methods: The tissue effect of laser incisions (CO2 laser: 3030 W/cm2; Nd:YAG-laser: 3180 W/cm2) on the lymphatics of the buccal mucosa in 60 Wistar rats was studied by light microscopy, histochemistry, and lymphography during surgery and subsequent wound healing.

[Establishment and characterization of 2 cell lines of squamous epithelial carcinoma of the mouth floor and tongue].

Objective: The cultivation of squamous cell carcinoma cells of the upper aerodigestive tract is more difficult compared to other malignancies because of microbial contamination. The establishment of new squamous cell carcinoma cell lines is absolutely necessary to further characterize this tumor entity.

Methods: Two squamous cell carcinoma cell lines of the floor of the mouth and the tongue were established from tumor biopsies.

[Results and surgical experiences in web transsection of hypopharyngeal (Zenker) diverticulum with the CO2 laser].

Since 1984 at the Department of Otorhinolaryngology, Head and Neck Surgery, University of Kiel, 34 patients with hypopharyngeal diverticular have been operated microendoscopically using the CO2 laser. Results and complications of this technique were compared to a group of patients in whom standard (non-laser) diverticulotomy was carried out from 1976 to 1993. Ninety per cent of the patients treated endoscopically with laser surgery have not had complaints and the rate of postoperative complications has been low.

[Effect of various culture media on growth behavior of human squamous epithelial carcinoma cells of the upper aerodigestive tract].

Sera containing media are frequently used for cultivation of epithelial cells, but the influence of serum on analytic methods can be significant. For this reason the development of serum-free media is of great significance. We investigated the influence of serum-free medium on growth characteristics of human squamous cell carcinoma (SCC) cells of the upper aerodigestive tract and compared these in serum-containing media, using cloned SCC cell lines HLaC-78 and HCFMK-1.

[The laryngeal lymph vessel system of the human. A morphologic and lymphography study with clinical viewpoints].

Laryngeal carcinomas have different metastatic characteristics depending on their locations. Although these changes could be explained by differences in density of the lymphatic system, studies of the laryngeal lymphatic system have been controversial. To clarify these differences the lymphatic systems of 184 organ specimens were investigated with light and electron microscopy, histochemistry and injection techniques.

[Serum soluble interleukin 2 receptor in patients with squamous epithelial carcinomas of the upper aerodigestive tract].

Background: Several investigations showed increased levels of the soluble interleukin-2 receptor (sIL-2 R) in chronic and malignant diseases. A correlation between elevated sIL-2 R levels and disease activity or tumor size was observed in some patients. At present, there are no studies of sIL-2 R serum levels in patients with squamous cell carcinomas of the upper aerodigestive tract.

DNA amplification for the in vitro detection of Candida albicans in head and neck squamous cell carcinomas.

DNA was extracted from whole cells of Candida albicans and digested with HindIII restriction enzyme. After electrophoresis in a segment of the lane containing between 800 and 1200 base pairs (bp) of DNA fragments, a 1.1-kilobase (kb) fragment was found that hybridizes to biopsied tumor cells from head and neck squamous cell carcinomas (SCC).