The impact of carbon and oxygen in alpha-titanium: ab initio study of solution enthalpies and grain boundary segregation.

The solution, grain boundary (GB) segregation, and co-segregation of carbon and oxygen atoms in α-titanium are studied using density functional theory. For five titanium tilt boundaries, including T1, T2, and C1 twin systems, we determine the GB structure, as well as GB energy and excess volume. The segregation energies and volumes of carbon and oxygen are calculated for 23 inequivalent interstitial voids, while for co-segregation 75 configurations are considered.

[Characteristics of isogenic variants of Bacillus anthracis with various content of virulence plasmids].

Aim: Production and characteristics by main cultural-morphologic and antigenic properties of isogenic variants Bacillus anthracis, that differ by the presence of virulence plasmids.

Materials And Methods: B. anthracis 81/1, 575/122 virulent and B.

[Epidemiologic situation of West Nile fever in Russia in 2010].

Problem of tropical arbovirus infection--West Nile fever (WNF)--spread in Russian Federation is discussed. Biology of WNF is discussed, WNF sources and reservoir are characterized. WNF outbreaks in Russia during the past 2 decades are presented in detail.

[Species-specific sera against surface antigens of Bacillus anthracis strains].

The species-related specificity of sera against 94-KD proteins isolated from culture filtrates of B. anthracis strains with different levels of virulence plasmids was studied to determine whether they might be used to identify the pathogen of anthrax. Sera against fractions 1 of culture filtrates of B.

[Experience with sevofluorane and halothane used during general anesthesia in children].

The investigators made a prospective analysis of the specific features of anesthesias with sevoflurane and halothane in 70 children aged 1 to 11 years with systemic surgical diseases and assessed their physical status as ASA Class I. The anesthetics sevoflurane, 3.1 +/- 0.

[Pathogenicity of West Nile virus: molecular markers].

The review describes the phenotypic properties, structure, and expression pattern of West Nile virus genome (Flaviviridae, Flavivirus, Japanese encephalitis antigenic complex), as well as the clinical picture and pathogenesis of its etiologically related disease West Nile fever. It also analyzes the available data on the impact of genetic mutations in the genome on the biological properties of the virus.

[Identification of the agents of coccidioidomycosis using polymerase chain reaction].

Two pairs of primers for diagnosis of coccidioidomycosis using the method of PCR were constructed. One pair was used for identification of the two species of Coccidioides (C. immitis and C.

[Venezuelan equine encephalitis: state-of-the-art].

The paper provides the currently available data on the global prevalence of Venezuelan equine encephalitis (VEE), its epidemiology, clinical picture, and specific prevention in human beings. It also discussed the problem of potential use of the causative agent of VEE as a subject of bioterrorism.

[Induction of Bacillus anthracis hemolytic activity with the use of Myxococcus xanthus].

The development of a new method for the induction of the B. anthracis hemolytic activity with the use of M. xanthus and the differentiation of pure cultures of the causative agent of anthrax from those contaminated with myxobacteria is presented.

[The effect of heterologous bacilli on the infectious process in anthrax in an experiment].

The possibility of the survival of guinea pigs after the simultaneous administration of different doses of Bacillus anthracis virulent strain 81/1 in combination with both B. anthracis vaccine strain STI and heterologous bacilli (B. subtilis strain PAC) has been experimentally shown.

[The characteristics of the interaction of Bacillus anthracis with host phagocytes in relation to the plasmid spectrum of the causative agent].

The study revealed that after the intraperitoneal inoculation of spores of B. anthracis strains with different plasmid composition into guinea pigs the active germination of the spores both outside and inside the cells of the host occurred as early as on hour 2 of their interaction with the macroorganism. The further fate of the infective agent and the character of its interaction with peritoneal exudate cells depended on the plasmid composition of the bacilli.

[A species-specific DNA probe for identifying toxic strains of anthrax pathogens].

On the plasmid DNA pOX01 of the anthrax pathogen two BamHI fragments were localized which facilitate detection of the Bacillus anthracis strains carrying pXO1 replicon. These fragments, after complete hydrolysis of plasmid DNA by HindIII, were cloned on the vector plasmids pUC19 and pBR322 by the "shot-gun" method in Escherichia coli cells. It is shown that the 900 bp BamHI/HindIII fragment from the pZAT1 recombinant plasmid has an ability for specific hybridization with DNA of toxigenic strains of B.

[Dimorphism in the fungus Coccidioides immitis Rixford et Gilchrist under the action of saprophytic bacilli].

It is shown that there are specific substances produced and secreted into the environment by saprotrophic bacilli. These inhibit the growth of the coccidioidal fungus in its mycelial form and some cells are converted into the yeast form, which leads to the destruction of the fungi (in natural environment) or, if the conditions allow, to their growth in the yeast form. This phenomenon, existence of a large amount of bacilli antagonistic to Coccidioides immitis, may be one of reasons why the latter has not been isolated so far from the soil in the territory of the USSR.

Immunobiological characteristic of antigens from the parasitic phase of Coccidiodes immitis.

The possibility of obtaining serologically active antigens from tissue spherules extracted from the lungs of albino mice infected with C. immitis has been demonstrated. Unlike the method of obtaining spherules in vitro, the accumulation of sufficient quantities of tissue spherules does not involve special difficulties.