RNA interference‑mediated inhibition of survivin and VEGF in pancreatic cancer cells in vitro.

The aim of the present study was to investigate the effects of simultaneous short hairpin RNA (shRNA)‑targeted survivin and vascular endothelial growth factor (VEGF) inhibition on the proliferation, apoptosis and angiogenesis of human pancreatic cancer cells (Panc‑1). Targeted small interfering RNA (siRNA) expression vectors of survivin and VEGF were constructed and transfected into Panc‑1 cells. The downregulation of survivin and VEGF expression was evaluated by real‑time PCR and western blot analysis.