Some Human Anti-Glycan Antibodies Lack the Ability to Activate the Complement System.

Naturally occurring human antibodies against glycans recognize and quickly eliminate infectious bacteria, viruses and aberrantly glycosylated neoplastic malignant cells, and they often initiate processes that involve the complement system. Using a printed glycan array (PGA) containing 605 glycoligands (oligo- and polysaccharides, glycopeptides), we examined which of the glycan-binding antibodies are able to activate the complement system. Using this PGA, the specificities of antibodies of the IgM and IgG classes were determined in the blood serum of healthy donors (suggested as mostly natural), and, then, using the same array, it was determined which types of the bound immunoglobulins were also showing C3 deposition.

Chemical Resolution of an Epitope Recognized by Blood Group Antibodies Capable of Binding Both A and B Red Blood Cells.

The high specificity of human antibodies to blood group A and B antigens is impressive, especially when considering the structural difference between these antigens (tetrasaccharides) is a NHAc versus a hydroxyl group on the terminal monosaccharide residue. It is well established that in addition to anti-A and anti-B there is a third antibody, anti-A,B capable of recognizing both A and B antigens. To analyze this AB specificity, we synthesized a tetrasaccharide, where the NHAc of the A antigen was replaced with an NH.

Turbulent burning velocity and thermodiffusive instability of premixed flames.

Reported in the paper are results of unsteady three-dimensional direct numerical simulations of laminar and turbulent, lean hydrogen-air, complex-chemistry flames propagating in forced turbulence in a box. To explore the eventual influence of thermodiffusive instability of laminar flames on turbulent burning velocity, (i) a critical length scale Λ_{n} that bounds regimes of unstable and stable laminar combustion is numerically determined by gradually decreasing the width Λ of computational domain until a stable laminar flame is obtained, and (ii) simulations of turbulent flames are performed by varying the width from Λ<Λ_{n} (in this case, the instability is suppressed) to Λ>Λ_{n} (in this case, the instability may grow). Moreover, simulations are performed either using mixture-averaged transport properties (low Lewis number flames) or setting diffusivities of all species equal to heat diffusivity of the mixture (equidiffusive flames), with all other things being equal.

Simple criterion of importance of laminar flame instabilities in premixed turbulent combustion of mixtures characterized by low Lewis numbers.

By (i) highlighting the mitigation effect of strain rates on laminar flame instabilities and (ii) comparing peak growth rates of laminar flame instabilities with strain rates generated by small-scale turbulent eddies, a simple criterion of importance of the influence of the instabilities on an increase in premixed flame surface area in turbulent flows is suggested. The criterion implies that, even in lean hydrogen-air mixtures, laminar flame instabilities can significantly affect the flame area only in weak or moderate turbulence (the Karlovitz number defined using laminar flame speed, thermal flame thickness, and Kolmogorov time scale is on the order of 10 or less under room conditions).

Smallest scale of wrinkles of a Huygens front in extremely strong turbulence.

By analyzing the statistically stationary stage of propagation of a Huygens front in homogeneous, isotropic, constant-density turbulence, a length scale l_{0} is introduced to characterize the smallest wrinkles on the front surface in the case of a low constant speed u_{0} of the front when compared to the Kolmogorov velocity u_{K}. The length scale is derived following a hypothesis of dynamical similarity that highlights a balance between (i) creation of a front area due to advection and (ii) destruction of the front area due to propagation. Consequently, the front speed is compared with the magnitude of the fluid velocity difference in two points separated by a distance smaller than the Kolmogorov length scale.

Surface-averaged quantities in turbulent reacting flows and relevant evolution equations.

While quantities conditioned to an isosurface of reaction progress variable c, which characterizes fluid state in a turbulent reacting flow, have been attracting rapidly growing interest in the recent literature, a mathematical and physical framework required for research into such quantities has not yet been elaborated properly. This paper aims at filling two fundamental gaps in this area, i.e.

INCREASED FORMATION OF PHOSPHORYLATED H2AX FOCI IN NUCLEI OF CELLS INFECTED BY HEPATITIS B AND B+D VIRUSES.

Liver cirrhosis and hepatocellular carcinoma are the most common outcomes of chronic hepatitis B. Hepatitis B virus (HBV) induces transformation and cell death in chronic hepatitis B (CHB). DNA double strand breaks (DSBs) represent the most dangerous type of genome damage.

A DNS Study of Closure Relations for Convection Flux Term in Transport Equation for Mean Reaction Rate in Turbulent Flow.

The present work aims at modeling the entire convection flux in the transport equation for a mean reaction rate in a turbulent flow, which (equation) was recently put forward by the present authors. In order to model the flux, several simple closure relations are developed by introducing flow velocity conditioned to reaction zone and interpolating this velocity between two limit expressions suggested for the leading and trailing edges of the mean flame brush. Subsequently, the proposed simple closure relations for are assessed by processing two sets of data obtained in earlier 3D Direct Numerical Simulation (DNS) studies of adiabatic, statistically planar, turbulent, premixed, single-step-chemistry flames characterized by unity Lewis number.

LC/MS at the whole protein level: Studies of biomolecular structure and interactions using native LC/MS and cross-path reactive chromatography (XP-RC) MS.

Interfacing liquid chromatography (LC) with electrospray ionization (ESI) to enable on-line MS detection had been initially implemented using reversed phase LC, which in the past three decades remained the default type of chromatography used for LC/MS and LC/MS/MS studies of protein structure. In contrast, the advantages of other types of LC as front-ends for ESI MS, particularly those that allow biopolymer higher order structure to be preserved throughout the separation process, enjoyed relatively little appreciation until recently. However, the past few years witnessed a dramatic surge of interest in the so-called "native" (with "non-denaturing" being perhaps a more appropriate adjective) LC/MS and LC/MS/MS analyses within the bioanalytical and biophysical communities.

THE ROLE OF DNA-METHYLTRANSFERASES IN THE LIFE CYCLE OF HEPATITIS B VIRUS AND PATHOGENESIS OF CHRONIC HEPATITIS B.

Chronic hepatitis B is caused by a persistent form of hepatitis B virus, covalently closed circular DNA (cccDNA). Stability of cccDNA is associated with intracellular localization of cccDNA and formation of minichromosome, regulated by epigenetic mechanisms. One of the key mechanisms in epigenetics is methylation of DNA on CpG islands.

Turbulent diffusion of chemically reacting flows: Theory and numerical simulations.

The theory of turbulent diffusion of chemically reacting gaseous admixtures developed previously [T. Elperin et al., Phys.

[Overexpression of DNA-methyltransferases in persistency of cccDNA pool in chronic hepatitis B].

Aim: To define the role of DNA-methyltransferases of type 1 and type 3A in hepatitis B viral cycle.

Material And Methods: Human hepatoma cells HepG2 with stable expression of 1.1-mer HBV genome were transfected with vectors encoding DNA-methyltransferase 1 (DNMT1), DNA-methyltransferase 3A (DNMT3A) or were co-transfected with these vectors.

Direct numerical simulation study of statistically stationary propagation of a reaction wave in homogeneous turbulence.

A three-dimensional (3D) direct numerical simulation (DNS) study of the propagation of a reaction wave in forced, constant-density, statistically stationary, homogeneous, isotropic turbulence is performed by solving Navier-Stokes and reaction-diffusion equations at various (from 0.5 to 10) ratios of the rms turbulent velocity U^{'} to the laminar wave speed, various (from 2.1 to 12.

Does Density Ratio Significantly Affect Turbulent Flame Speed?

In order to experimentally study whether or not the density ratio substantially affects flame displacement speed at low and moderate turbulent intensities, two stoichiometric methane/oxygen/nitrogen mixtures characterized by the same laminar flame speed = 0.36 m/s, but substantially different were designed using (i) preheating from = 298 to 423 K in order to increase , but to decrease , and (ii) dilution with nitrogen in order to further decrease and to reduce back to the initial value. As a result, the density ratio was reduced from 7.

Analytical and numerical study of travelling waves using the Maxwell-Cattaneo relaxation model extended to reaction-advection-diffusion systems.

Within the framework of the Maxwell-Cattaneo relaxation model extended to reaction-diffusion systems with nonlinear advection, travelling wave (TW) solutions are analytically investigated by studying a normalized reaction-telegraph equation in the case of the reaction and advection terms described by quadratic functions. The problem involves two governing parameters: (i) a ratio φ^{2} of the relaxation time in the Maxwell-Cattaneo model to the characteristic time scale of the reaction term, and (ii) the normalized magnitude N of the advection term. By linearizing the equation at the leading edge of the TW, (i) necessary conditions for the existence of TW solutions that are smooth in the entire interval of -∞<ζ<∞ are obtained, (ii) the smooth TW speed is shown to be less than the maximal speed φ^{-1} of the propagation of a substance, (iii) the lowest TW speed as a function of φ and N is determined.

Speed selection for traveling-wave solutions to the diffusion-reaction equation with cubic reaction term and Burgers nonlinear convection.

The problem of traveling wave (TW) speed selection for solutions to a generalized Murray-Burgers-KPP-Fisher parabolic equation with a strictly positive cubic reaction term is considered theoretically and the initial boundary value problem is numerically solved in order to support obtained analytical results. Depending on the magnitude of a parameter inherent in the reaction term (i) the term is either a concave function or a function with the inflection point and (ii) transition from pulled to pushed TW solution occurs due to interplay of two nonlinear terms; the reaction term and the Burgers convection term. Explicit pushed TW solutions are derived.