Laser-induced hyperthermia: comparison of two different methods.

The effectiveness of two different techniques of laser-induced hyperthermia was analyzed in healthy piglet livers. Hyperthermia was produced using a continuous-wave Nd:YAG laser with both a contact sapphire probe and a bare quartz fibre. In both methods the tip was inserted into the liver, and the temperature 1 cm from the tip was raised and held between 42 degrees C and 44 degrees C for 600 seconds.

Comparison of surgical lasers and conventional methods in skin incisions.

Steel scalpel, electrocautery, CO2 laser used in a continuous wave mode (CW) and rapid superpulse mode (RSP), and contact Nd: YAG laser were tested on pig skin incisions. Speed of incision and histological changes near the wounds were examined. Light microscopical observations were made on postoperative day 0 using standard Van Gieson stain.

Lactate dehydrogenase in laser incisions: a comparative analysis of skin wounds made with steel scalpel, electrocautery, superpulse--continuous wave mode carbon-dioxide lasers, and contact Nd:YAG laser.

A histochemical method for demonstrating lactate dehydrogenase activity was used in addition to standard Van Gieson stain to study early alterations near wounds made in pig skin by steel scalpel, electrocautery, two modes of CO2 laser (the rapid super-pulse mode and the continuous wave mode), and contact Nd:YAG laser. The enzyme-free zone near the wounds made using the thermal knives appeared to be twice as wide as the necrotic zone observed with Van Gieson stain. In polarized light, the enzyme-free area showed two zones of equal width with respect to birefringence of collagen fibers.

Flow cytometric analysis of the DNA profile of renal cell carcinoma.

DNA flow cytometry was applied to one hundred consecutive renal cell carcinomas from years 1974-1979. Single cell suspensions were prepared from paraffin-embedded tissue. From 96 evaluable tumours 34 were considered diploid and 62 aneuploid.

Comparison of dental information obtained from self-examination and clinical examination.

The validity of self-examination information regarding the number and distribution of remaining teeth, the presence of dentures and the validity of molar support construed professionally from the self-examination data was assessed from 93 persons. This was done by comparing self-examination information with data from clinical and radiographical examinations. Agreement between self-examination and clinical examination was found to be fairly good regarding the number of remaining teeth and the presence of removable dentures.

Ischaemic contracture in isolated rat heart: reversible or irreversible myocardial injury?

An isolated rat heart preparation was reperfused at 37 degrees C for 10 min after 10, 20, 30 and 40 min of ischaemia. The left ventricular tension was measured by means of a balloon catheter filled with water and connected to a pressure recorder. The left ventricular resting tension began to increase at 9 +/- 1 min (mean +/- SEM) and was maximally developed (myocardial contracture) at 18 +/- 1 min of ischaemia.

Renal oncocytoma: the incidence of 18 surgical and 12 autopsy cases.

The material of 562 surgically removed renal cell carcinomas or adenomas was reviewed, and 18 tumors were diagnosed as renal oncocytomas. The incidence seemed to increase strikingly during recent years. Similarly, among 112 renal cell tumours from 8489 autopsies, 12 oncocytomas were found.

Anaerobic glycolysis and the development of ischaemic contracture in isolated rat heart.

The relationship between myocardial ATP content and the increase in left ventricular resting tension during ischaemia (ischaemic contracture) was studied in isolated rat hearts perfused for 15 min either with aerobic buffer (pO2 greater than 500 mmHg) containing non-glycolytic substrate, acetate (5 mmol X litre-1), or with hypoxic buffer (pO2 less than 10 mmHg) with glucose (10 mmol X litre-1) before making them totally ischaemic for 10 min. ATP was determined spectrophotometrically from extracts of frozen whole hearts. Left ventricular tension was recorded by intraventricular balloon catheter.

Effect of verapamil on reperfusion damage and calcium paradox in isolated rat heart.

The effect of verapamil on ischaemic contracture, reperfusion injury after global ischaemia, and calcium paradox was studied in isolated rat heart. The development of ischaemic contracture was measured by a balloon catheter inserted into the left ventricle, filled with water, and connected to a pressure recorder. Myocardial perfusability was tested by infusing sodium fluorescein solution into the cannulated aortic root.

The relationship between ischemic contracture and no-reflow phenomenon in isolated rat heart.

The relationship between ischemic contracture and no-reflow phenomenon was studied in 59 isolated rat hearts during global ischemia. The contracture was measured by a water-filled balloon catheter placed in the left ventricular lumen. The time of onset of contracture was changed by preischemic infusion with buffer containing 0.

Effect of hypoxia on the microvascular function in isolated rat hearts.

The effect of hypoxia on the microvascular function in continuously perfused isolated rat hearts was studied. The development of hypoxic contracture was determined by measuring the left ventricular pressure or the decrease of the left ventricular volume. The myocardial perfusability was tested by infusing 0.

Ischaemic contracture and myocardial perfusion in isolated rat heart.

The development of left ventricular contracture and myocardial perfusion defect was studied in isolated rat hearts during global ischaemia of 90 min duration. The left ventricular pressure was measured by a balloon catheter inserted into the ventricle and filled with water. The pressure reached the maximum at 16 min of ischaemia.