Regeneration of adult rat sensory and motor neuron axons through chimeric peroneal nerve grafts containing donor Schwann cells engineered to express different neurotrophic factors.

Large peripheral nerve (PN) defects require bridging substrates to restore tissue continuity and permit the regrowth of sensory and motor axons. We previously showed that cell-free PN segments repopulated ex vivo with Schwann cells (SCs) transduced with lentiviral vectors (LV) to express different growth factors (BDNF, CNTF or NT-3) supported the regeneration of axons across a 1 cm peroneal nerve defect (Godinho et al., 2013).

Immunohistochemical, ultrastructural and functional analysis of axonal regeneration through peripheral nerve grafts containing Schwann cells expressing BDNF, CNTF or NT3.

We used morphological, immunohistochemical and functional assessments to determine the impact of genetically-modified peripheral nerve (PN) grafts on axonal regeneration after injury. Grafts were assembled from acellular nerve sheaths repopulated ex vivo with Schwann cells (SCs) modified to express brain-derived neurotrophic factor (BDNF), a secretable form of ciliary neurotrophic factor (CNTF), or neurotrophin-3 (NT3). Grafts were used to repair unilateral 1 cm defects in rat peroneal nerves and 10 weeks later outcomes were compared to normal nerves and various controls: autografts, acellular grafts and grafts with unmodified SCs.

Closed digital artery injury.

Closed digital artery injury with secondary acute ischemia is a rare phenomenon. The most common injury pattern involves a crush mechanism with a resultant transverse fracture in proximity to the interphalangeal joints. Secondary acute ischemia requires urgent surgical exploration with digital arterial repair to avoid necrosis and associated decreased hand function.

Localized myxedema of the foot associated with trauma and surgery.

Localized extratibial myxedema is a rare presentation of thyroid disease that manifests with varied symptoms. Previous surgical or radioiodine treatment of hyperthyroidism is linked to the development of localized myxedema, as is prior trauma or surgery. We present the first known case of localized foot myxedema on a background of Graves disease following a traumatic and surgical precipitant and compare and discuss similar cases found in a literature review.

Spontaneous common iliac artery dissection after exercise.

Purpose: To review the clinical features and management of spontaneous iliac dissections.

Case Report: A healthy 60-year-old competitive cyclist presented with acute onset of short-distance claudication following vigorous exercise. Angiography showed a dissection flap extending from the right common iliac artery to the external iliac artery.

Deep circumflex iliac artery as a cause of type II endoleak.

Purpose: To report a collateral pathway involving the deep circumflex iliac artery causing a type II endoleak following endoluminal exclusion of an abdominal aortic aneurysm (AAA).

Case Report: A 75-year-old man was investigated for a persistent type II endoleak 2 years after endovascular AAA repair with a Zenith Trifab stent-graft. Angiography revealed contrast in the sac from a lumbar artery fed via a collateral of the deep circumflex iliac artery.