Development of a visual detection method of porcine deltacoronavirus using loop-mediated isothermal amplification.

The emergence of porcine deltacoronavirus (PDCoV) presents a significant threat to both human and animal health due to its ability to cause highly contagious enteric diseases. This underscores the crucial need for timely and accurate diagnosis to facilitate effective epidemiological investigation and clinical management. This research aimed to establish a visual detection method based on reverse transcription loop-mediated isothermal amplification (RT-LAMP) for PDCoV testing.

A long-cycle-life reversible Li-CO2 battery enabled by engineering the active sites of graphene with Pd nanoparticles.

Li-CO2 batteries have been recognized as an emerging technology for energy storage systems owing to their high theoretical specific energy and environmentally friendly CO2 fixation ability. However, their development for applications requires a high energy efficiency and long cycle-life, this is currently limited to the formation of wide-bandgap insulator Li2CO3 during discharge. Here, nanoparticle Pd supported on reduced graphene oxide (rGO) is utilized as cathodes for Li-CO2 batteries, Pd nanoparticles as active centers significantly enhance CO2RR/CO2ER reaction activity, which can support the fast formation and decomposition of Li2CO3 in organic electrolytes and achieve a high discharge capacity of 7500 mAh g-1.

Halogenated Functional Electrolyte Additive for Li-CO Batteries.

In recent years, functional electrolyte additives have been widely studied during the CO evolution reaction (COER) and CO reduction reaction (CORR) processes for Li-CO batteries. Owing to different concerns, functions of these additives are also multiple and limited. In this work, the multiple impacts of functional electrolyte additives for Li-CO batteries are discussed.

A Rule for Response Sensitivity of Structural-Color Photonic Colloids.

To mimic natural photonic crystals having color regulation capacities dynamically responsive to the surrounding environment, periodic assembly structures have been widely constructed with response materials. Beyond monocomponent materials with stimulus responses, binary and multiphase systems generally offer extended color space and complex functionality. Constructing a rule for predicting response sensitivity can provide great benefits for the tailored design of intelligently responsive photonic materials.

Computationally-driven epitope identification of PEDV N-protein and its application in development of immunoassay for PEDV detection.

The nucleocapsid (N) protein is a suitable candidate for early diagnosis of porcine epidemic diarrhea virus (PEDV). Here, we identified the linear B-cell epitopes of the PEDV N-protein by integrating a computational-experimental framework and constructed three-dimensional (3D) structure model of the N protein using the ColabFold program in Google Colaboratory. Furthermore, we prepared the monoclonal antibodies against the predicted epitopes and recombinant N protein, respectively, and selected pairing mAbs (named 9C4 and 3C5) to develop a double-antibody sandwich immunochromatographic test strip using CdSe/ZnS quantum dots (QDs)-labelled 9C4 and 3C5 as capture and detection antibodies, respectively.

Sophisticated yet Convenient Information Encryption/Decryption Based on Synergistically Time-/Temperature-Resolved Photonic Inks.

Exploring high-safety but convenient encryption and decryption technologies to combat threats of information leakage is urgently needed but remains a great challenge. Here, a synergistically time- and temperature-resolved information coding/decoding solution based on functional photonic inks is demonstrated. Encrypted messages can be stored into multiple channels with dynamic-color patterns, and information decryption is only enabled at appointed temperature and time points.

Research on Continuous Error Compensation of a Sub-Arc-Second Macro/Micro Dual-Drive Rotary System.

In this paper, a sub-arc-second macro/micro dual-drive rotary system is designed, and the continuous compensation of the system error and its experimental research are completed. First, the macro-drive system is driven by a direct-drive motor, and the micro-drive system uses a piezoelectric ceramic to drive the micro-drive rotary mechanism; the system uses a micro-drive system to compensate the motion error of the macro-drive system, and uses circular grating to feedback the displacement of the macro/micro total output turntable to form a macro/micro dual-drive closed-loop control system. Second, based on the establishment of the system error model, the design of the system's continuous error compensation scheme is completed.

Point-of-Care Tests for Rapid Detection of Porcine Epidemic Diarrhea Virus: A Systematic Review and Meta-Analysis.

The timely and accurate diagnosis of porcine epidemic diarrhea virus (PEDV) infection is crucial to reduce the risk of viral transmission. Therefore, the objective of this review was to evaluate the overall diagnostic accuracy of rapid point-of-care tests (POCTs) for PEDV. Studies published before 7 January 2022 were identified by searching PubMed, EMBASE, Springer Link, and Web of Science databases, using subject headings or keywords related to point of care and rapid test diagnostic for PEDV and PED.

The Cardiac Glycoside Deslanoside Exerts Anticancer Activity in Prostate Cancer Cells by Modulating Multiple Signaling Pathways.

Prostate cancer (PCa) is a leading cause of cancer-related deaths among men worldwide, and novel therapies for advanced PCa are urgently needed. Cardiac glycosides represent an attractive group of candidates for anticancer repurposing, but the cardiac glycoside deslanoside has not been tested for potential anticancer activity so far. We found that deslanoside effectively inhibited colony formation in vitro and tumor growth in nude mice of PCa cell lines 22Rv1, PC-3, and DU 145.

Positioning Performance of a Sub-Arc-Second Micro-Drive Rotary System.

In the macro/micro dual-drive rotary system, the micro-drive system compensates for the position error of the macro-drive system. To realize the sub-arc-second (i.e.