Developed BL-EF to acquire plant growth-promoting functions under salt stress by introducing the ACC deaminase gene.

The application of plant growth-promoting rhizobacteria (PGPR) is a novel and effective strategy to ameliorate soil salinity and increase agricultural productivity. ACC deaminase (ACCD) in PGPR plays a key role in alleviating salt stress and promoting plant growth. This study aimed to investigate the potential of ACCD-producing strain BL-EF to mitigate salt stress in tomato plants.

Combination with isopentenyl diphosphate isomerase gene affects expression of two linalool/nerolidol synthases isoforms from Lingzhi.

GlSTS21 and GsSTS41, derived from Ganoderma lucidum (Leyss. ex Fr.) Karst.

Tumor-intrinsic PRMT5 upregulates FGL1 methylating TCF12 to inhibit CD8 T-cell-mediated antitumor immunity in liver cancer.

Protein arginine methyltransferase 5 (PRMT5) acts as an oncogene in liver cancer, yet its roles and in-depth molecular mechanisms within the liver cancer immune microenvironment remain mostly undefined. Here, we demonstrated that disruption of tumor-intrinsic PRMT5 enhances CD8 T-cell-mediated antitumor immunity both and . Further experiments verified that this effect is achieved through downregulation of the inhibitory immune checkpoint molecule, fibrinogen-like protein 1 (FGL1).

Omics Analysis Revealing Flavonoid Content During Maize Grain Germination.

The germination process initiates an active process of secondary metabolism, which produces a series of secondary metabolites, including flavonoids. A metabolomics and transcriptomics analysis was conducted on maize grains germinated at three different stages. A total of 374 metabolites were detected in maize grains.

Clinical characteristics and outcomes of surgical resection for brain metastases from lung adenocarcinoma.

Objective: The purpose of this study was to explore the clinical characteristics, survival time and prognostic factors of patients undergoing craniotomy for brain metastases (BM) from lung adenocarcinoma (LUAD).

Methods: A total of 208 patients with BM from LUAD who underwent craniotomy at the Zhengzhou University People's Hospital, Henan province, China from March 2005 to October 2022 were included in this retrospective study. All patients were confirmed as BM of LUAD by histopathology.

Radiomic signatures of brain metastases on MRI: utility in predicting pathological subtypes of lung cancer.

Background: The pathological sub-classification of lung cancer is crucial in diagnosis, treatment and prognosis for patients. Quick and timely identification of pathological subtypes from imaging examinations rather than histological tests could help guiding therapeutic strategies. The aim of the study is to construct a non-invasive radiomics-based model for predicting the subtypes of lung cancer on brain metastases (BMs) from multiple magnetic resonance imaging (MRI) sequences.

Effects of different additives on fermentation quality, mycotoxin concentrations, and microbial communities in high-moisture corn kernels during wet storage.

Introduction: The moisture content of corn kernels at harvest in China is relatively high, and wet storage effectively preserves high-moisture corn kernels. However, ensuring effective fermentation during storage is crucial.

Methods: To address this, we systematically investigated the variations in fermentation quality, mycotoxin concentrations, and microbial community composition under different additive treatments.

Solvent-mediated analgesia via the suppression of water permeation through TRPV1 ion channels.

Activation of the ion channel transient receptor potential vanilloid 1 (TRPV1), which is integral to pain perception, leads to an expansion of channel width, facilitating the passage of cations and large organic molecules. However, the permeability of TRPV1 channels to water remains uncertain, owing to a lack of suitable tools to study water dynamics. Here, using upconversion nanophosphors to discriminate between HO and DO, by monitoring water permeability across activated TRPV1 at the single-cell and single-molecule levels, and by combining single-channel current measurements with molecular dynamics simulations, we show that water molecules flow through TRPV1 and reveal a direct connection between water migration, cation flow and TRPV1 functionality.

Distribution, transport and ecological risk prediction of organophosphate esters in China seas based on machine learning.

Organophosphate esters (OPEs), widely used globally, have been detected in significant amounts in various environmental media, raising concerns about their persistence, bioaccumulation, and associated risks. Traditional sampling and detection methods are time-consuming and labor-intensive, limiting a comprehensive understanding. This study employs Extreme Gradient Boosting (XGBoost) and Light Gradient Boosting Machine (LGBM) models, using 12 feature variables and 463 OPEs concentration data points, to investigate the distribution and ecological risk of total OPEs (T-OPEs), chlorinated alkyl OPEs (Cl-OPEs), and aryl-OPEs in seawater of China Seas.

Parishin Alleviates Pulmonary Fibrosis by Reducing CD38 Levels in Naturally Aging Mice.

Parishin, a natural compound, has demonstrated significant potential in mitigating age-related phenotypes and improving outcomes in age-associated diseases. Given that aging is a major risk factor for numerous chronic conditions, including pulmonary fibrosis, we investigated parishin's effects on cellular senescence and lung health. In our study, we treated mouse lung epithelial cells with parishin and observed a reduction in cellular senescence markers alongside an upregulation of sirtuin 1 (SIRT1).

Integrated analysis of single-cell RNA-seq and bulk RNA-seq reveals mediated expression patterns by distinct tumor microenvironment immune profiles in cervical cancer.

Few studies have analyzed the effect of () expression patterns on the tumor microenvironment (TME) during development of cervical cancer (CC). We elucidated the landscape and score of expression in CC using single-cell RNA sequencing (scRNA-seq) and RNA sequencing datasets. Further, we aimed the MMPscore to probe the infiltration of immune cells.

Psychotropic medication and hepatobiliary health: Ultrasound observations on patients with schizophrenia.

Objective: This study sought to examine the association between antipsychotic drug use and hepatobiliary health based on serum markers and ultrasound observations on a sample of patients with schizophrenia compared to age and gender matched healthy controls.

Methods: The 120 patients with schizophrenia and 60 control subjects had their blood drawn to measure liver function tests and underwent hepatobiliary ultrasonography to determine hepatobiliary lesions. Liver function tests included total cholesterol (TC), triglycerides (TG), alanine aminotransferase (ALT), and aspartate aminotransferase (AST).

Study on the prebiotic effects of insoluble crude and fine fibers of wheat bran after simulated digestion in vitro.

This study aims to evaluate the probiotic effects of insoluble crude and fine fibers of wheat bran on the intestine after simulated in vitro digestion. It was found that the particle size distribution of modified fine wheat bran (MWB) was significantly smaller than that of natural crude wheat bran (NWB). MWB had a looser texture and more porous structure.

Ferroptosis-Related Gene Signatures in Epilepsy: Diagnostic and Immune Insights.

Epilepsy is characterized by a multifaceted aetiology. Ferroptosis has recently been implicated in seizure pathophysiology, although its mechanistic role in epilepsy remains obscure. We examined the roles of ferroptosis-related genes (FRGs) in epilepsy cohorts from the GSE143272 dataset.

PKMYT1 knockdown inhibits cholesterol biosynthesis and promotes the drug sensitivity of triple-negative breast cancer cells to atorvastatin.

Triple negative breast cancer (TNBC) as the most aggressive molecular subtype of breast cancer is characterized by high cancer cell proliferation and poor patient prognosis. Abnormal lipid metabolism contributes to the malignant process of cancers. Study observed significantly enhanced cholesterol biosynthesis in TNBC.

Association between Branched-Chain amino acids and Epilepsy: A Mendelian randomized study.

Background: Branched-chain amino acids (BCAAs) have been affected epilepsy, yet conclusions remain inconclusive, lacking causal evidence regarding whether BCAAs affect epilepsy. Systematic exploration of the causal relationship between BCAAs and epilepsy could hand out new ideas for the treatment of epilepsy.

Methods: Utilizing bidirectional Mendelian randomization (MR) study, we investigated the causal relationship between BCAA levels and epilepsy.

Differences and Mechanism of Waxy Corn Starch and Normal Corn Starch in the Preparation of Recrystallized Resistant Starch (RS3).

This study prepared resistant starch (RS) from waxy corn starch and normal corn starch and analyzed the effects of its molecular and microstructural characteristics on RS content. The RS content of waxy corn resistant starch (RS-WCS) was highest at 57.8%, whereas that of normal corn resistant starch (RS-NCS) was 41.

Exosomes derived from hTERT-immortalized cells delay cellular senescence of human fibroblasts.

hTERT gene therapies hold significant promise for treating age-related diseases. However, further research is required to address the challenges of delivery and ethical considerations. We hypothesized that exosomes derived from hTERT-immortalized cells could function similarly to hTERT gene therapies by maintaining telomere length and attenuating cellular senescence biomarkers.

Lower serum creatinine to cystatin C ratio associated with increased incidence of frailty in community-dwelling elderly men but not in elderly women.

Background: Sarcopenia has been reported to play an important role in frailty syndrome. The serum creatinine/serum cystatin C ratio (Scr/Cys C ratio) has recently been recognized as a valuable indicator for assessing sarcopenia. However, few studies have examined the association between serum creatinine/serum cystatin C ratio and frailty.

Wearable, Biocompatible, and Dual-Emission Ocular Multisensor Patch for Continuous Profiling of Fluoroquinolone Antibiotics in Tears.

The abuse or misuse of antibiotics in clinical and agricultural settings severely endangers human health and ecosystems, which has raised profound concerns for public health worldwide. Trace detection and reliable discrimination of commonly used fluoroquinolone (FQ) antibiotics and their analogues have consequently become urgent to guide the rational use of antibiotic medicines and deliver efficient treatments for associated diseases. Herein, we report a wearable eye patch integrated with a quadruplex nanosensor chip for noninvasive detection and discrimination of primary FQ antibiotics in tears during routine eyedrop treatment.

Automatic detection of pulmonary embolism on computed tomography pulmonary angiogram scan using a three-dimensional convolutional neural network.

Objective: To propose a convolutional neural network (EmbNet) for automatic pulmonary embolism detection on computed tomography pulmonary angiogram (CTPA) scans and to assess its diagnostic performance.

Methods: 305 consecutive CTPA scans between January 2019 and December 2021 were enrolled in this study (142 for training, 163 for internal validation), and 250 CTPA scans from a public dataset were used for external validation. The framework comprised a preprocessing step to segment the pulmonary vessels and the EmbNet to detect emboli.