Publications by authors named "Linley R Pierce"
Article Synopsis
- - Norovirus is a major cause of gastroenteritis globally, and its ability to infect cells is influenced by the arrangement of lipids in cell membranes, although this connection has not been fully explored.
- - Research shows that the protein TMEM30a, which is part of lipid flippases, is essential for the replication of murine norovirus (MNV) and helps the virus bind and enter host cells.
- - The study reveals that lipid asymmetry in cell membranes aids in MNV infection and persistence, challenging previous assumptions about cell markers, as certain lipids do not inhibit the virus but are instead linked to its successful entry.
Article Synopsis
- Trim7 is an E3 ubiquitin ligase that can influence host-viral interactions, showing both pro-viral and anti-viral roles in cell culture by targeting viral proteins for degradation.
- In a study involving two mouse lines lacking Trim7, researchers found no significant changes in viral load or tissue spread of murine norovirus (MNV) during infection, indicating that Trim7 may not play a crucial role in MNV infection in vivo.
- The investigation also showed that removing innate immune responses like STING and STAT1 did not uncover any regulatory function of Trim7 on MNV replication, suggesting that classifying Trim7 as a broad-spectrum antiviral may be misleading.
Noroviruses are a leading cause of gastroenteritis worldwide, yet the molecular mechanisms of how host antiviral factors restrict norovirus infection are poorly understood. Here, we present a CRISPR activation screen that identifies mouse genes which inhibit murine norovirus (MNV) replication. Detailed analysis of the major hit Trim7 demonstrates a potent inhibition of the early stages of MNV replication.
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