Chitosan/Sodium Tripolyphosphate/Sodium Alginate/miRNA-34c-5p Antagomir Scaffolds Promote the Functionality of Rabbit Cranial Parietal Repair.

Purpose: MicroRNA-34c-5p (miR-34c-5p) plays a pivotal role in bone remodeling, yet its therapeutic potential is hindered by challenges such as instability, limited cellular internalization, and immune responses. This study was aimed at developing innovative scaffolds capable of efficiently delivering microRNAs (miRNAs), specifically miR-34c-5p.

Methods: Chitosan (CS)/sodium tripolyphosphate (STPP)/sodium alginate (SA) scaffolds, referred to as CTS scaffolds, were synthesized at a specific ratio and characterized using dynamic light scattering and scanning electron microscopy (SEM).

-glycosylation of SCAP exacerbates hepatocellular inflammation and lipid accumulation via ACSS2-mediated histone H3K27 acetylation.

Sterol regulatory element binding protein (SREBP) cleavage-activating protein (SCAP) is a widely expressed membrane glycoprotein that acts as an important modulator of lipid metabolism and inflammatory stress. -glycosylation of SCAP has been suggested to modulate cancer development, but its role in nonalcoholic steatohepatitis (NASH) is poorly understood. In this study, the -glycosylation of SCAP was analyzed by using sequential trypsin proteolysis and glycosidase treatment.

Periodontal ligament cells-derived exosomes promote osteoclast differentiation via modulating macrophage polarization.

Several studies have demonstrated that exosomes (Exos) are involved in the regulation of macrophage polarization and osteoclast differentiation. However, the characteristics as well as roles of exosomes from human periodontal ligament cells (hPDLCs-Exos) in M1/M2 macrophage polarization and osteoclast differentiation remain unclear. Here, periodontal ligament cells were successfully extracted by method of improved Type-I collagen enzyme digestion.

Synthesis of X@DRHC (X=Co, Ni, Mn) catalyst from comprehensive utilization of waste rice husk and spent lithium-ion batteries for efficient peroxymonosulfate (PMS) activation.

Highly efficient resource recycling and comprehensive utilization play a crucial role in achieving the goal of reducing resource wasting, environmental protection, and achieving goal of sustainable development. In this work, the two kinds waste resources of agricultural rice husk and metal ions (Co, Ni, and Mn) from spent lithium-ion batteries have been skillfully utilized to synthesize novel Fenton-like catalysts. Desiliconized rice husk carbon (DRHC) with rich pore structure and large specific surface area from rice husk has been prepared and used as scalable carrier, and dandelion-like nanoparticles cluster could be grown in situ on the surface of the carrier by using metal ions contained waste water.

Effect of Mechanical Loading on Bone Regeneration in HA/β-TCP/SF Scaffolds Prepared by Low-Temperature 3D Printing .

It has been well demonstrated that a dynamic culture environment improves tissue-engineered bone formation , but little is known about how cyclical mechanical loading induced bone formation in scaffolds . To mimic the organic and inorganic components and multilevel structure of a bony microenvironment, hydroxyapatite/β tricalcium phosphate/silk fibroin(HA/β-TCP/SF) composite scaffolds with macro- and micropores were fabricated in this study. The mechanical properties and structure of the scaffolds were adjusted based on the ratio of organic and inorganic components and three-dimensional (3D) printing parameters.

Respiratory Fluid Mechanics of the Effect of Mouth Breathing on High-Arched Palate: Computational Fluid Dynamics Analyses.

Computational fluid dynamics (CFD) was introduced into the study of palate growth and development to explain the mechanisms by which mouth breathing affects palate descent from an aerodynamic perspective. Cone beam computed tomography (CBCT) data were used to reconstruct a 3-dimensional model during natural mouth breathing of a volunteer. The model was imported into CFX 19.

Hepatocyte CD36 protects mice from NASH diet-induced liver injury and fibrosis via blocking N1ICD production.

Background & Aims: Fatty acid translocase CD36 (CD36/FAT) is a widely expressed membrane protein with multiple immuno-metabolic functions. Genetic CD36 deficiency is associated with increased risk of metabolic dysfunction-associated fatty liver disease (MAFLD) in patients. Liver fibrosis severity mainly affects the prognosis in patients with MAFLD, but the role of hepatocyte CD36 in liver fibrosis of MAFLD remains unclear.

Hepatocyte CD36 modulates UBQLN1-mediated proteasomal degradation of autophagic SNARE proteins contributing to septic liver injury.

Macroautophagy/autophagy plays a protective role in sepsis-induced liver injury. As a member of class B scavenger receptors, CD36 plays important roles in various disorders, such as atherosclerosis and fatty liver disease. Here we found that the expression of CD36 in hepatocytes was increased in patients and a mouse model with sepsis, accompanied by impaired autophagy flux.

Periodontal ligament fibroblasts-derived exosomes induced by PGE inhibit human periodontal ligament stem cells osteogenic differentiation via activating miR-34c-5p/SATB2/ERK.

Several studies have confirmed that exosomes containing microRNAs (miRNAs) from the aseptic inflammatory microenvironment play an important role in bone remodeling. But the mechanism that induces changes in the osteogenic ability of periodontal ligament stem cells (PDLSCs) is still unclear. In the present study, the osteogenic function of periodontal ligament fibroblasts-derived exosomes induced by PGE on PDLSCs was detected by real-time PCR, alizarin red assay and alkaline phosphatase staining.

Clinical effectiveness of different types of bone-anchored maxillary protraction devices for skeletal Class III malocclusion: Systematic review and network meta-analysis.

Objective: This study aimed to estimate the clinical effects of different types of bone-anchored maxillary protraction devices by using a network meta-analysis.

Methods: We searched seven databases for randomized and controlled clinical trials that compared bone-anchored maxillary protraction with tooth-anchored maxillary protraction interventions or untreated groups up to May 2021. After literature selection, data extraction, and quality assessment, we calculated the mean differences, 95% confidence intervals, and surface under the cumulative ranking scores of eleven indicators.

Clinical effects of maxillary protraction in different stages of dentition in skeletal class III children: A systematic review and meta-analysis.

Objectives: This systematic review and meta-analysis aimed to evaluate the effectiveness of using maxillary protraction during different stages of the dentition by assessing changes in the jaws and inclination of incisors.

Materials And Methods: MEDLINE (PubMed), Embase, Cochrane, Web Of Science, China National Knowledge Infrastructure and Wanfang Databases were searched without time limitations up to 15 January 2022. Google Scholar was used to search grey literature.

Prognostic analysis and nomogram establishment in patients with head and neck myoepithelial carcinoma.

Purpose: To further explore the clinicopathological characteristics and determinants of survival of patients with HNMC.

Methods: The Surveillance, Epidemiology and End Results (SEER) database was used to collect the data of patients diagnosed with HNMC from 1975 to 2016. Kaplan-Meier analysis and log-rank testing compared the survival difference.

Low torque is a risk factor for non-carious cervical lesions (NCCLs) in maxillary premolars.

Purpose: To determine the prevalence of non-carious cervical lesions (NCCLs) in maxillary premolars of different torques and simulated cervical stress profiles of the premolars under coincident loadings using finite element analysis (FEA).

Methods: The CBCT scans of 616 maxillary premolars from 154 subjects were retrospectively evaluated. The premolars were ascribed into low torque group (LTG) <-10.

Novel Prediction Models for Patients With Oral Squamous Cell Carcinoma at Different Anatomical Sites.

Purpose: The individualized prediction of oral cavity squamous cell carcinoma (OC-SCC) is essential and should be as comprehensive as possible. The aim of this study was to identify new risk factors and develop nomograms comparing all anatomic sites of the oral cavity.

Materials And Methods: We performed a retrospective cohort study using the Surveillance, Epidemiology, and End Results (SEER) database.

The association between nutritive, non-nutritive sucking habits and primary dental occlusion.

Background: The development of primary dentition can be affected by oral sucking habits. Therefore, this study aims to investigate the association of nutritive and non-nutritive sucking habits with primary dentition development.

Methods: One thousand one hundred and fourteen children aged 2 to 5 years old in Hong Kong were recruited in a cross-sectional study.

Serum Bisphenol A is an independent risk factor of hyperuricemia: A 6-year prospective study.

Objective: This study aims to evaluate whether serum Bisphenol A (BPA) is a risk factor for hyperuricemia.

Methods: In this prospective study, a total of 482 participants without hyperuricemia were enrolled at baseline and followed up for 6 years. Clinical characteristics were recorded, and serum levels of uric acid and BPA were measured.

Bisphenol A promotes hyperuricemia via activating xanthine oxidase.

The prevalence of hyperuricemia has increased rapidly over the past decades. Bisphenol A (BPA) is an environmental endocrine disruptor. We investigated the effects of BPA on uric acid metabolism and its potential mechanisms.

Influence of different intensities of vibration on proliferation and differentiation of human periodontal ligament stem cells.

Introduction: To understand the effects of low-magnitude, high-frequency (LMHF) mechanical vibration at different intensities on human periodontal ligament stem cell (hPDLSC) proliferation and osteogenic differentiation.

Material And Methods: The effect of vibration on hPDLSC proliferation, osteogenic differentiation, tenogenic differentiation and cytoskeleton was assessed at the cellular, genetic and protein level.

Results: The PDLSC proliferation was decreased after different magnitudes of mechanical vibration; however, there were no obvious senescent cells in the experimental and the static control group.

Association of breastfeeding and three-dimensional dental arch relationships in primary dentition.

Background: The benefits of breastfeeding on oral health are still inconclusive, especially the association on occlusion. This study aimed to investigate the association of breastfeeding and the development of primary dentition.

Methods: A cross-sectional study was conducted with 851 Asian children aged 2-5 years old in Hong Kong.

The role of traditional Chinese medicines in osteogenesis and angiogenesis.

The article aims to review various Traditional Chinese Medicines (TCMs) with both osteogenic and angiogenic effects, alone and in combination, and to consider whether these TCMs promote osteogenesis via angiogenesis and vascular endothelial growth factor (VEGF). Each of the TCMs involving in osteogenesis was searched through PubMed and CBMdisc using its Latin name and English name, and keywords such as 'osteogenesis', 'bone', 'osteoblast', 'angiogenesis', 'VEGF' were used. A total of 241 articles were screened from PubMed and CBMdisc.

Up-regulated osteogenic transcription factors during early response of human periodontal ligament stem cells to cyclic tensile strain.

Introduction: As one group of periodontal ligament (PDL) cells, human periodontal ligament stem cells (hPDLSCs) have been isolated and identified as mesenchymal adult stem cells (MSCs) since 2004. It has been well accepted that PDL sensitively mediates the transmission of stress stimuli to the alveolar bone for periodontal tissue remolding. Besides, the direction of MSCs differentiation has been verified regulated by mechanical signals.

The role of vascular endothelial growth factor in ossification.

Osteogenesis and angiogenesis are two closely correlated processes during bone growth, development, remodelling and repair.Vascular endothelial growth factor (VEGF) is an essential mediator during the process of angiogenesis. Based on an extensive literature search, which was carried out using the PubMed database and the keywords of osteogenesis, VEGF, endochondral ossification and intramembranous ossification, this manuscript reviews the role of VEGF in ossification, with emphasis on its effect in endochondral and intramembranous ossification.