Publications by authors named "Linkai Zhu"
Article Synopsis
- A thorough understanding of molecular and cellular processes during peri-implantation is crucial for improving pregnancy outcomes, which is when most pregnancy failures happen.
- Researchers analyzed single-cell transcriptomes of bovine embryos at different peri-implantation stages (days 12, 14, 16, and 18) to identify cell types and gene expression patterns in important embryonic structures.
- The study unveiled a new primitive trophoblast lineage and highlighted similarities and differences in developmental processes between bovines and other mammals, emphasizing the importance of cell communication for healthy early development.
Article Synopsis
- Single-cell analysis of bovine blastocyst transcriptome has identified key cell lineages, including the inner cell mass (ICM), trophectoderm (TE), and transitional cells.
- Comparing embryos derived from in vivo (IVV) and two different in vitro (IVC and IVR) protocols revealed delays in cell fate commitment to ICM in IVC and IVR embryos, affecting their development potential.
- Pathway analysis showed that IVC embryos had reduced cellular signaling and transport activities, leading to lower developmental potential, while IVR embryos exhibited increased signaling but issues with ion balance, resulting in compromised development compared to IVV embryos.
In Brief: Inadequate maternal nutrition during gestation can have immediate and lifelong effects on offspring. This study shows that maternal restricted - and over- nutrition during gestation do not affect semen characteristics in F1 male offspring but alters offspring sperm sncRNA profiles and DNA methylome in sheep.
Abstract: There is a growing body of evidence that inadequate maternal nutrition during gestation can have immediate and lifelong effects on offspring.
Unlabelled: Supporting healthy pregnancy outcomes requires a comprehensive understanding of the cellular hierarchy and underlying molecular mechanisms during peri-implantation development. Here, we present a single-cell transcriptome-wide view of the bovine peri-implantation embryo development at day 12, 14, 16 and 18, when most of the pregnancy failure occurs in cattle. We defined the development and dynamic progression of cellular composition and gene expression of embryonic disc, hypoblast, and trophoblast lineages during bovine peri-implantation development.
View Article and Find Full Text PDFProfiling transcriptome at single cell level of bovine blastocysts derived in vivo (IVV), in vitro from conventional culture medium (IVC), and reduced nutrient culture medium (IVR) has enabled us to reveal cell lineage segregation, during which forming inner cell mass (ICM), trophectoderm (TE), and an undefined population of transitional cells. Only IVV embryos had well-defined ICM, indicating in vitro culture may delay the first cell fate commitment to ICM. Differences between IVV, IVC and IVR embryos were mainly contributed by ICM and transitional cells.
View Article and Find Full Text PDFHere, we report that a chemical cocktail (LCDM: leukemia inhibitory factor [LIF], CHIR99021, dimethinedene maleate [DiM], minocycline hydrochloride), previously developed for extended pluripotent stem cells (EPSCs) in mice and humans, enables de novo derivation and long-term culture of bovine trophoblast stem cells (TSCs). Bovine TSCs retain developmental potency to differentiate into mature trophoblast cells and exhibit transcriptomic and epigenetic (chromatin accessibility and DNA methylome) features characteristic of trophectoderm cells from early bovine embryos. The bovine TSCs established in this study will provide a model to study bovine placentation and early pregnancy failure.
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- Researchers conducted advanced ribosome profiling on bovine oocytes and early embryos to understand how translation changes during early development.
- They identified four distinct modes of how different types of mRNAs are translated, with a focus on low-abundance transcripts vital for metabolic processes and mitochondrial function.
- They observed that while translation patterns matched transcription during the oocyte and two-cell stages, a significant shift occurred in the eight-cell stage, reflecting the onset of embryonic genome activation.
Blockchain Data Secure Transmission Method Based on Homomorphic Encryption.
Comput Intell Neurosci
May 2022
To ensure the security of data transmission and recording in Internet environment monitoring systems, this paper proposes a study of a secure method of blockchain data transfer based on homomorphic encryption. Blockchain data transmission is realized through homomorphic encryption. Homomorphic encryption can not only encrypt the original data, but also ensure that the data result after decrypting the data is the same as the original data.
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- * Researchers discovered an oocyte-specific lncRNA that exists in two variants and is found in the nucleus and cytoplasm of oocytes and embryos.
- * Overexpressing this lncRNA in oocytes did not alter meiotic maturation, but knocking it down led to issues with oocyte cytokinesis and embryonic development, indicating its crucial role in reproductive success.
The epigenome is dynamic and forged by epigenetic mechanisms, such as DNA methylation, histone modifications, chromatin remodeling, and non-coding RNA species. Increasing lines of evidence support the concept that certain acquired traits are derived from environmental exposure during early embryonic and fetal development, i.e.
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