Improved performance of hip DXA using a novel region of interest in the upper part of the femoral neck: in vitro study using bone strength as a standard of reference.

We tested the hypothesis that bone mineral density (BMD) and bone mineral content (BMC) in proximal human femur specimens in the upper neck region of interest (ROI) and femoral neck axis length (FNAL) provide a significantly better prediction of femoral bone strength than standard ROIs in vitro. BMD and BMC were measured in 110 proximal femur specimens using a standard dual-energy X-ray absorptiometry (DXA) scanner. The analysis included a new ROI in the upper neck as well as the standard ROIs.

MR imaging findings in the follow-up of patients with different stages of knee osteoarthritis and the correlation with clinical symptoms.

Objective: To assess the rate of cartilage loss, the change in bone marrow edema pattern and internal joint derangement at 1.5-T MRI in patients with knee osteoarthritis and to correlate these findings with the clinical Western Ontario and McMaster University Osteoarthitis (WOMAC) score.

Methods: Forty subjects (mean age 57.

3.0 vs 1.5 T MRI in the detection of focal cartilage pathology--ROC analysis in an experimental model.

Objective: To use receiver operator characteristics (ROC) analysis for assessing the diagnostic performance of three cartilage-specific MR sequences at 1.5 and 3 T in detecting cartilage lesions created in porcine knees.

Design: Eighty-four cartilage lesions were created in 27 porcine knee specimens at the patella, the medial and lateral femoral and the medial and lateral tibial cartilage.

In vivo 3T spiral imaging based multi-slice T(1rho) mapping of knee cartilage in osteoarthritis.

T(1rho) describes the spin-lattice relaxation in the rotating frame and has been proposed for detecting damage to the cartilage collagen-proteoglycan matrix in osteoarthritis. In this study, a multi-slice T(1rho) imaging method for knee cartilage was developed using spin-lock techniques and a spiral imaging sequence. The adverse effect of T(1) regrowth during the multi-slice acquisition was eliminated by RF cycling.

Buprenorphine diffusion: the attitudes of substance abuse treatment counselors.

In October 2002, the Food and Drug Administration approved buprenorphine for use in the treatment of opioid dependence. Successful diffusion, adoption, and implementation of this medication within the treatment field depend in part on substance abuse counselors. Using questionnaire data obtained from 2,298 counselors in community-based treatment programs in the private and public sectors between June 2002 and July 2004, we explored the diffusion of this new treatment technique.

Current diagnostic techniques in the evaluation of bone architecture.

Bone quality, which encompasses trabecular bone and cortical bone architecture, bone mineralization, turnover, and microdamage, is an essential component of bone strength. Therefore, bone quality, bone density, and total content are the most important parameters in the diagnosis of osteoporosis. Noninvasive assessment of bone quality has recently received considerable attention because bone density alone is not a surrogate for fracture prevalence and occurrence, and does not completely explain the therapeutic efficacy of emerging treatments.

Bone imaging: traditional techniques and their interpretation.

Patients with osteoporosis have to be diagnosed at an early stage to prevent fractures, the worst complication of this disease. Currently, measurements of bone mineral density (BMD) are used most frequently in the diagnosis of osteoporosis. According to the World Health Organization, osteoporosis is defined on the basis of BMD measurements that are compared with those of a healthy, young, female population.

Normal and pathological MR findings in osteochondral autografts with longitudinal follow-up.

The purpose of this study was to analyze normal and pathological MR findings in osteochondral autograft transfer systems (OATS) of the knee and ankle in the longitudinal follow-up and in relation to the clinical findings. MR imaging was performed in 55 patients (21 females: 34 males; mean age 34.5+/-12.

Feasibility of in vivo structural analysis of high-resolution magnetic resonance images of the proximal femur.

Previously, high resolution MRI to assess bone structure of deep-seated regions of the skeleton such as the proximal femur was substantially limited by signal-to-noise ratio (SNR). With the advent of new optimized pulse sequences in MRI at 1.5 T and 3 T, it may now be possible to depict and quantify the trabecular microarchitecture in the proximal femur.

Cartilage MR imaging at 3.0 versus that at 1.5 T: preliminary results in a porcine model.

Purpose: To compare 1.5- and 3.0-T magnetic resonance (MR) images of porcine knee specimens containing artificial cartilage lesions in terms of accuracy of lesion depiction, image quality, and signal-to-noise ratio (SNR).

Recombinant tumor-associated MUC1 glycoprotein impairs the differentiation and function of dendritic cells.

Tumors exploit several strategies to evade immune recognition, including the production of a large number of immunosuppressive factors, which leads to reduced numbers and impaired functions of dendritic cells (DCs) in the vicinity of tumors. We have investigated whether a mucin released by tumor cells could be involved in causing these immunomodulating effects on DCs. We used a recombinant purified form of the MUC1 glycoprotein, an epithelial associated mucin that is overexpressed, aberrantly glycosylated, and shed during cancer transformation.

Optimization of gadodiamide concentration for MR arthrography at 3 T.

Objective: The purpose of our study was to determine the optimal concentration of a gadolinium-based contrast agent (gadodiamide) for direct MR arthrography at 3 T compared with 1.5 T in an in vitro study.

Conclusion: Optimized concentrations of gadolinium-based contrast agents for MR arthrography are similar at 3 and 1.

Computer-aided quantification of focal cartilage lesions using MRI: accuracy and initial arthroscopic comparison.

Objective: The purpose of the study was to validate a Gradient Peak Method (GPM) by evaluating its accuracy and consistency at different magnetic field strengths. The GPM using magnetic resonance imaging (MRI) was previously proposed to quantitatively assess the morphology of focal cartilage lesions, and its feasibility was demonstrated.

Methods: GPM quantifies the morphologic properties of cartilage lesions based on their three-dimensional geometry.

Microbiology of middle ear effusions from 292 patients undergoing tympanostomy tube placement for middle ear disease.

Objective: Otitis media is one of the most commonly diagnosed childhood illnesses. Ideally, culture directed therapy for otitis media would be available, however, the common approach is to treat infections with antibiotics that cover the most common pathogens. The objective of this study is to describe the pathogens cultured from the middle ear effusions (MEE) of patients that underwent tympanostomy tube placement for middle ear disease, compare these results with previous studies, and assess for trends suggestive of changes in the microbiology of these patients.

Retinopathy from a green laser pointer: a clinicopathologic study.

Objective: To report retinopathy following exposure to light from a commercially available class 3A green laser pointer.

Methods: A 55-year-old woman with a ring melanoma was scheduled for enucleation. The eye (visual acuity 20/20) had a healthy-appearing macular retina.

Quantification of trabecular bone structure using magnetic resonance imaging at 3 Tesla--calibration studies using microcomputed tomography as a standard of reference.

The purpose of this study is to use high-resolution magnetic resonance (MR) imaging at 3 Tesla (3T) to quantify trabecular bone structure in vitro using femoral head specimens, and to correlate the calculated structure measures with those that were determined using microcomputed tomography (microCT), the standard of reference. Fifteen cylindrical cores were obtained from fresh femoral heads after total hip arthroplasty. MR images were obtained at 3T using a transmit-receive wrist coil.

Radiologic assessment of osteoporotic vertebral fractures: diagnostic and prognostic implications.

As populations age osteoporosis becomes an increasingly important public health issue. Among osteoporotic fractures vertebral fractures are of particular relevance: they are the most common fractures, frequently are asymptomatic but have an important influence on prognosis and morbidity in the osteoporotic patient. Previous studies have suggested that these fractures are frequently not diagnosed and that radiologists miss a high percentage of osteoporotic, vertebral fractures present on lateral chest radiographs.

Detection of hepatocellular carcinoma: comparison of Gd-DTPA- and ferumoxides-enhanced MR imaging.

The aim was to compare the diagnostic performance of dynamic Gd-DTPA- and ferumoxides-enhanced MRI for hepatocellular carcinoma (HCC). Twenty-five patients with chronic hepatitis or liver cirrhosis underwent both dynamic gadopentetate- and ferumoxides-enhanced MRI studies of the liver for HCC detection on the same day. MR data of both studies were retrospectively and independently analyzed.

Characterization of a novel NADP(+)-dependent D-arabitol dehydrogenase from the plant pathogen Uromyces fabae.

We have identified and characterized a novel NADP(+)-dependent D-arabitol dehydrogenase and the corresponding gene from the rust fungus Uromyces fabae, a biotrophic plant pathogen on broad bean (Vicia faba). The new enzyme was termed ARD1p (D-arabitol dehydrogenase 1). It recognizes D-arabitol and mannitol as substrates in the forward reaction, and D-xylulose, D-ribulose and D-fructose as substrates in the reverse reaction.

Trabecular bone structure obtained from multislice spiral computed tomography of the calcaneus predicts osteoporotic vertebral deformities.

Purpose: To compare multislice computed tomography (MSCT)-derived parameters of the trabecular bone structure of the calcaneus with bone mineral density (BMD) in their ability to differentiate between donors with and without osteoporotic fractures of the spine and to optimize CT scan protocols.

Methods: Forty-two postmortem calcanei (81.2 +/- 10 years) were imaged with a 16-detector row MSCT system using 4 different scan protocols varying spatial resolution (12-24 lp/cm) and radiation dose.

Gender differences in trabecular bone architecture of the distal radius assessed with magnetic resonance imaging and implications for mechanical competence.

High-resolution magnetic resonance imaging (hrMRI) has recently made it possible to evaluate trabecular bone structure in vivo. Despite obvious gender differences in fracture incidence at the distal radius, little is known about gender differences in trabecular bone microarchitecture and its relationship to the structural strength of the forearm. The aim of this study was to determine trabecular bone structure in the distal radius of elderly women and men and its correlation with failure loads of the distal radius as determined in a fall configuration.

Chest radiography with a digital flat-panel detector: experimental receiver operating characteristic analysis.

Purpose: To evaluate the influence of different detector radiation doses and peak kilovoltage settings on diagnostic performance and radiation dose at posteroanterior (PA) chest radiography performed with an amorphous silicon flat-panel detector (FPD).

Materials And Methods: All examinations were performed by using a digital FPD. PA chest radiographs of an anthropomorphic chest phantom were obtained with detector radiation doses of 2.

Comparison of multislice CT arthrography and MR arthrography for the detection of articular cartilage lesions of the elbow.

The objective of this study was to compare the value of multislice CT arthrography and MR arthrography in the assessment of cartilage lesions of the elbow joint. Twenty-six cadaveric elbow specimens were examined with the use of CT arthrography and MR arthrography prior to joint exploration and macroscopic inspection of articular cartilage. Findings at CT and MR arthrography were compared with macroscopic assessments in 104 cartilage areas.

Possible roles for mannitol and mannitol dehydrogenase in the biotrophic plant pathogen Uromyces fabae.

Levels of the C6-polyol mannitol were observed to rise dramatically in the biotrophic interaction of the rust fungus Uromyces fabae and its host plant Vicia faba. Mannitol was found in millimolar concentrations in extracts and apoplastic fluids of infected leaves and also in extracts of spores. We suggest that this polyol might have at least a dual function: first, as a carbohydrate storage compound, and second, as a scavenger of reactive oxygen species.

Migration of iron oxide-labeled human hematopoietic progenitor cells in a mouse model: in vivo monitoring with 1.5-T MR imaging equipment.

Purpose: To evaluate the use of clinical 1.5-T magnetic resonance (MR) imaging equipment to depict the in vivo distribution of iron oxide-labeled human hematopoietic progenitor cells in athymic mice.

Materials And Methods: This study was approved by the ethical committee, and all women had given consent to donate umbilical cord blood for research.