To overcome the problems of easy aggregation, poor reproducibility and homogeneity of metal nanosols, a SERS substrate with good sensitivity, homogeneity and reproducibility was designed and prepared for the detection of disease markers in urine. Silver nanocubes (Ag NCs) were firstly prepared and then dispersed in cationic cellulose (C-CNF) to form a homogeneous gel, which was dropped on a filter paper to develop a substrate with good SERS activity. This substrate combines the superior SERS properties of Ag NCs with the stability of C-CNF and has a minimum detection concentration of 10 M for R6G.
View Article and Find Full Text PDFis a complex genus in the family Malvaceae that has high ecological and economical values. Owing to the lack of sufficient distinguishable morphological and molecular characteristics, interspecific relationships in this genus are not clear. Chloroplast (cp) genomes are small, meanwhile most angiosperms usually undergo matrilineal inheritance.
View Article and Find Full Text PDFThe objective of this research was to characterize the genetic diversity and phylogenetic diversity among 12 cattle breeds (10 Chinese breeds and two foreign taurine breeds as controls) utilizing gene mtDNA 16S rRNA. The complete sequences of the mtDNA 16S rRNA genes of the 251 animals were 1570 bp long. The mean percentages of the four nitrogen bases were 37.
View Article and Find Full Text PDFPCR-RFLP was applied to analyze the polymorphisms of POU1F1 gene in 218 Qinchuan cattle (QQ) and Chinese Holstein cattle (HC). Results demonstrated Hinf I polymorphisms in the 451 bp PCR product in the two populations. The frequencies of alleles A/B in QQ and HC populations were 0.
View Article and Find Full Text PDF