Cross-species transmission of a novel bisegmented orfanplasmovirus in the phytopathogenic fungus .

Mycoviruses have been found in various fungal species across different taxonomic groups, while no viruses have been reported yet in the fungus In this study, a novel orfanplasmovirus, namely Exserohilum rostratum orfanplasmovirus 1 (ErOrfV1), was identified in the strain JZ1 from maize leaf. The complete genome of ErOrfV1 consists of two positive single-stranded RNA segments, encoding an RNA-dependent RNA polymerase and a hypothetical protein with unknown function, respectively. Phylogenetic analysis revealed that ErOrfV1 clusters with other orfanplasmoviruses, forming a distinct phyletic clade.