Enantioselective disruption of circadian rhythm behavior in goldfish (Carassius auratus) induced by chiral fungicide triadimefon at environmentally-relevant concentration.

The pollution of triadimefon (TDF) fungicides significantly hinders the "One Health" frame achievement. However, the enantioselective effects of chiral TDF on the circadian rhythm of fish remained unclear. Herein, TDF enantiomers (R(-)-TDF and S(+)-TDF) and racemic Rac-TDF were selected to investigate their enantioselective effects and mechanisms on circadian rhythm of goldfish (Carassius auratus) at an environmentally-relevant concentration (100 µg L⁻¹).

Phthalates esters disrupt demersal fish behavior: Unveiling the brain-gut axis impact.

The widespread use of plasticizers like phthalate esters (PAEs) has led to environmental and health concerns. The neurobehavioral toxicity of these compounds in marine environments, particularly regulated by the "brain-gut" axis, remains unclear, especially concerning wild demersal fish of high ecological value. Our investigation into the behavioral effects of three common PAEs, i.

Mechanistic Insight into Receptor-Mediated Delivery of Cationic-β-Cyclodextrin:Hyaluronic Acid-Adamantamethamidyl Host:Guest pDNA Nanoparticles to CD44(+) Cells.

Targeted delivery is a key element for improving the efficiency and safety of nonviral vectors for gene therapy. We have recently developed a CD44 receptor targeted, hyaluronic acid-adamantamethamidyl based pendant polymer system (HA-Ad), capable of forming complexes with cationic β-cyclodextrins (CD-PEI(+)) and pDNA. Complexes formed using these compounds (HA-Ad:CD-PEI(+):pDNA) display high water solubility, good transfection efficiency, and low cytotoxicity.

Efficient pDNA Delivery Using Cationic 2-Hydroxypropyl-β-Cyclodextrin Pluronic-Based Polyrotaxanes.

A family of cationic Pluronic-based polyrotaxanes (PR(+)), threaded with 2-hydroxypropyl-β-cyclodextrin (HPCD), was synthesized for pDNA delivery into multiple cell lines. All PR(+) formed highly stable, positively charged pDNA complexes that were < 250 nm in diameter. The cellular uptake and pDNA transfection efficiencies of the PR(+):pDNA complexes was enhanced relative to the commercial transfection standards L2K and bPEI, while displaying similar or lower toxicity profiles.