Mass production of cation-exchange cryogels and their chromatographic adsorption performance for bioseparation.

The preparation of cryogels with enhanced protein adsorption capabilities holds significant promise in bioseparation. The challenge of industrializing cryogels lies in achieving efficient large-scale production while maintaining controllable performance characteristics. In this work, 200 of poly (hydroxyethyl methacrylate) (pHEMA) monolithic cryogels were mass-produced per batch by cryo-polymerization.

Salt-tolerant and thermostable mechanisms of an endoglucanase from marine Aspergillus niger.

The cellulase cocktail of marine Aspergillus niger exhibited salt-tolerant and thermostable properties, which is of great potential in industrial application. In order to excavate the single tolerant cellulase components from complex cellulase cocktail, constitutive homologous expression was employed for direct obtainment of the endoglucanase (AnEGL). Enzymatic property study revealed that AnEGL exhibited a property of salt tolerance and a strong thermostability in high salinity environment.

Directed expression of halophilic and acidophilic β-glucosidases by introducing homologous constitutive expression cassettes in marine Aspergillus niger.

The hydrolysis step by β-glucosidase (BGL) is generally recognized as the major limiting step in cellulose degradation and the BGLs with prominent enzymatic properties are of great importance for efficient utilization of lignocellulosic biomass. In order to identify some salt-tolerant BGLs, two BGL genes were cloned from marine Aspergillus niger ZJUBE-1 genome. Then two bgl expression cassettes driven by gpdA promoter were respectively transformed into marine A.