No relationship between HTLV-1 infection and filariasis--Serological study on patients with filariasis in Recife, Brazil.

The seroprevalence of antibodies against human T-cell leukemia virus was determined by ELISA in 68 patients with filarial infestation living in an endemic area. The total seropositivity was 2.9% and the HTLV-1-positive cases were detected in 2 microfilaremic patients 12 and 40 years old.

Electrophoretically mediated micro-assay of alkaline phosphatase using electrochemical and spectrophotometric detection in capillary electrophoresis.

Electrophoretically mediated micro-assays of alkaline phosphatase (ALP) are demonstrated in capillary electrophoretic systems using both electrochemical (ED) and spectrophotometric (UV) detection. In the ED mode, p-aminophenylphosphate was used as the substrate and p-aminophenol (pAP) was monitored at a carbon fiber electrode held at +180 mV vs. Ag/AgCl.

Pharmacokinetic studies using microdialysis probes in subcutaneous tissue: effects of the co-administration of ethanol and acetaminophen.

Loop geometry microdialysis probes with membrane lengths of 40-60 mm were used to monitor the effects of acute and chronic doses of ethanol on acetaminophen pharmacokinetics in awake, freely-moving rats. Microdialysis probes used in this configuration provide very high concentration recoveries and good precision at flow rates below 2 microliters min-1. The ability of microdialysis to monitor pharmacokinetics in subcutaneous tissue and blood vessels is compared.

Prevalence of human herpesvirus 6 antibody among isolated Amazonian Amerindian communities in Brazil.

Human herpesvirus 6 (HHV6) antibody was detected by the anticomplement immunofluorescence test in 65 of 592 serum samples (10.9%) obtained from Amerindians belonging to 4 remote communities inhabiting the Amazon Region of Brazil. Seropositivity rates ranged from 5.

Human T cell leukemia virus type 1 (HTLV-1) antibodies in healthy populations and renal transplanted patients in the north-east of Brazil.

The seroprevalence of human T cell leukemia virus type 1 (HTLV-1) infection was investigated in Brazilians (570): native inhabitants (298) and descendants from Japanese (272) living in Recife and its neighborhoods--North-east of Brazil. Furthermore, polytransfused renal transplanted patients (54) were also examined for the serological status to this virus. The seropositivity to HTLV-1, screened by enzyme-linked immunosorbent assay (ELISA), was low: 1.

Determination of endogenous ions in intercellular fluid using capillary ultrafiltration and microdialysis probes.

Capillary ultrafiltration probes are novel sampling tools for continuously monitoring small molecules in the extracellular fluid of awake animals. Capillary ultrafiltration uses a vacuum applied to hydrophilic membrane fibres and extracts intercellular fluid and quantitatively recover many small hydrophilic molecules. The effects of continuously removing a small amount of fluid from the interstitial space are not known.

Cytomegalovirus, human herpesvirus 6 and human T cell leukemia virus infection in renal transplanted patients in the northeast of Brazil.

The seroprevalence of antibodies against cytomegalovirus (CMV), human herpesvirus 6 (HHV-6) and human T cell leukemia virus type 1 (HTLV-1), determined by ELISA and by fluorescence in 54 renal transplanted patients, was 96.2%, 88.8% and 11.

Pharmacokinetic studies using micellar electrokinetic capillary chromatography with in vivo capillary ultrafiltration probes.

Micellar electrokinetic capillary chromatography (MECC) is shown to be a quantitative method for the determination of theophylline in capillary ultrafiltrates of biological systems. MECC exhibits reproducibility in migration times of 1.3% relative standard deviation (R.

Pharmacokinetic monitoring in subcutaneous tissue using in vivo capillary ultrafiltration probes.

Capillary ultrafiltration probes were utilized for in vivo sampling of therapeutic drugs in awake rats. Capillary ultrafiltration probes implanted into subcutaneous tissue were able to follow the disposition of acetaminophen and theophylline. Ultrafiltration probes provided samples at a rate of 2-3 microL/min.

Capillary ultrafiltration: in vivo sampling probes for small molecules.

Capillary ultrafiltration is a novel sampling method convenient for low molecular weight substances in living biological systems. By application of a negative pressure across a hydrophilic membrane capillary, small molecules are actively "pulled" across the membrane and collected. By elimination of large molecules and cellular matter, the ultrafiltrate collected is well suited for further analysis by liquid chromatography, capillary electrophoresis, or mass spectrometry.

In vivo sampling using loop microdialysis probes coupled to a liquid chromatograph.

Microdialysis probes with longer membranes (20-100 mm) provide increased relative recovery over traditional shorter probes (1-4 mm) developed for neuroscience applications. The characterization and optimization of "straight through" or "loop type" probes for use in subcutaneous tissue are considered. Membrane area, probe size, inlet and outlet tubing dimensions, and flow-rate are examined for their effects on relative recovery, the total collection rate, and bulk flow through the membrane wall.

Nitrosyl hemoglobin: EPR components at low temperatures.

The EPR spectrum of nitrosyl hemoglobin has been studied from 7.5 K to 104 K. It is composed of at least three components (A, B and C) which have a different dependence on temperature and power level.

E.p.r. studies of photolysis of nitrosyl haemoglobin at low temperatures: effects of quaternary structure.

Photolysis of nitrosyl haemoglobin (HbNO) has been studied from 6.5 K to 20 K for different NO saturation conditions. The kinetic curves are fitted equally well by a biphasic exponential and a distribution of activation energies.

Seroprevalence of human herpesvirus 6 infection in Brazilian and Japanese populations in the north-east of Brazil.

The seroprevalence of human herpesvirus 6 (HHV-6) infection was examined for 434 Brazilians and 250 Japanese immigrants living in Recife and its vicinity, in the North-East of Brazil. A total of 684 sera from the healthy individuals were screened for IgG antibodies to HHV-6 by anticomplement immunofluorescence (ACIF) test. The seropositivity rate to HHV-6 showed little difference between the two groups: namely, it was 76.

E.p.r. studies of photolysis of nitrosyl haemoglobin at low temperatures.

Photolysis of HbNO has been studied from 6.2 K to 15.5 K by electron spin resonance during and after continuous illumination.

Prevalence of cytomegalovirus antibodies in Brazilian and Japanese populations in the north-east of Brazil.

The serological status to cytomegalovirus (CMV) was examined for 616 Brazilians and 399 Japanese immigrants living in the North-East of Brazil. The sera were screened for IgG antibodies to CMV by enzyme-linked immunosorbent assay (ELISA). The overall prevalence of CMV antibodies was higher in the Japanese population (83.