[Correlation analysis of the effect of postoperative drainage on surgical efficacy of thoracolumbar fractures through multifidus interspace approach].

Objective: To investigate correlation of the effect factors on surgical efficacythe of whether postoperative drainage through the multifidus interspace approach in the treatment of thoracolumbar fractures.

Methods: A total of 66 patients with thoracolumbar fractures admitted from January 2017 to December 2021 were retrospectively analyzed. According to whether postoperative drainage, 36 patients were in drainage group and 30 patients were in no drainage group.

's role in RANKL-induced osteoclast differentiation: insights from a bone marrow macrophage model.

Background: Osteoporosis (OP) is a chronic disease characterized by decreased bone mass, loss of skeletal structural integrity and increased susceptibility to fracture. Available studies have shown that the pyruvate dehydrogenase kinase (PDK) family is associated with osteoclastogenesis and bone loss, but the specific role of in bone pathology has not been systematically investigated.

Methods: A cell OP model was established in receptor activator for nuclear factor-B Ligand (RANKL)-induced bone marrow macrophages (BMMs).

De Garengeot Hernia, an acute appendicitis in the right femoral hernia canal, and successful management with transabdominal closure and appendectomy: a case Report.

Incarceration of the appendix within a femoral hernia is a rare condition of abdominal wall hernia about 0.1 to 0.5% in reported femoral hernia [1].

[Effects of oral and intravenous tranexamic acid on perioperative blood loss after lumbar spinal canal decompression and fusion].

Objective: To explore the effects of different administration methods of tranexamic acid(TXA) on the perioperative blood loss, hidden blood loss, transfusion rate and adverse reactions in lumbar spinal decompression and fusion.

Methods: Sixty patients who received lumbar spinal canal decompression and fusion from July 2019 to July 2020 were enrolled and divided into observation group and control group, with 30 cases in each group. The observation group was given 2 g TXA orally at 2 hours before operation, control group was given 1 g TXA for 5-10 min before skin incision and 6 hours after operation intravenously.

Cytoprotective effects of myristicin against hypoxia‑induced apoptosis and endoplasmic reticulum stress in rat dorsal root ganglion neurons.

The aim of the present study was to investigate the role of myristicin (Myr; 1‑allyl‑5‑methoxy‑3,4‑methylenedioxybenzene), an active aromatic compound isolated from nutmeg, carrot, basil, cinnamon and parsley, in hypoxia‑induced apoptosis in rat dorsal root ganglion (DRG) neurons. It was observed that Myr significantly enhanced cell viability in hypoxia‑induced DRG neurons in a dose‑dependent manner; the optimal concentration of Myr was 50 µM. Furthermore, Myr reduced the percentage of deoxynucleotidyl transferase‑mediated dUTP nick end‑labeling‑positive neuronal cells and influenced the expression of the pro‑apoptotic gene B‑cell lymphoma 2 (Bcl‑2) associated X protein, the apoptosis protease cleaved caspase‑3 and the anti‑apoptotic gene Bcl‑2, in the hypoxia‑induced group.

Synthesis, biological evaluation and molecular modeling of aloe-emodin derivatives as new acetylcholinesterase inhibitors.

A series of aloe-emodin derivatives were designed, synthesized and evaluated as acetylcholinesterase inhibitors. Most of the new prepared compounds showed remarkable acetylcholinesterase inhibitory activities. Among them, the compound 1-((4,5-dihydroxy-9,10-dioxo-9,10-dihydroanthracen-2-yl) methyl) pyridin-1-ium chloride (C3) which has a pyridinium substituent possessed the best inhibitory activity of acetylcholinesterase (IC(50)=0.

Effect of pcDNA3.1- vascular endothelial growth factor 165 recombined vector on vascular buds in rabbit vertebral cartilage endplate.

Background: This study aimed to investigate the effect of pcDNA3.1-vascular endothelial growth factor (VEGF)165 vector on vertebral cartilage endplate vascular buds and intervertebral discs.

Methods: Rabbits were randomly assigned to the control and experimental groups with 10 in each.

Intermittent Cyclic Mechanical Tension-Induced Calcification and downregulation of ankh gene expression of end plate chondrocytes.

Study Design: Intermittent Cyclic Mechanical Tension (ICMT) was applied to end plate chondrocytes by using an FX-4000T Flexercell Tension Plus unit (Flexcell International Corporation, Hillsborough, NC). Changes of end plate chondrocytes were observed after ICMT stimulation.

Objective: To investigate the relationship between mechanical stimulation and calcification of end plate chondrocytes.

[Effects of vascular endothelial growth factor vector on vascular buds of vertebral cartilaginous endplate in rabbits].

Objective: To directly inject recombinant pcDNA3.1-VEGF165 plasmid into degeneration intervertebral disc and explore its effects on vascular buds of vertebral cartilage endplate and intervertebral disc in rabbits.

Methods: Rabbits were randomly assigned into the experimental and control groups (n = 10 each).

Effects of mechanical strain on ANK, ENPP1 and TGF-β1 expression in rat endplate chondrocytes in vitro.

We investigated the effects of mechanical strain on the progressive ankylosis (ANK) gene and extracellular nucleotide phosphatase/phosphodiesterase (ENPP)1 mRNA expression and TGF-β1 protein expression in rat endplate chondrocytes in vitro. Endplate chondrocytes were isolated and cultured in vitro. Following identification with toluidine blue and immunocytochemical staining, chondrocytes were subjected to 10% elongation with various frequencies (0.

Finite element analysis of the screw in percutaneous axial lumbosacral interbody fusion.

Objective: To establish a three-dimensional finite element model of the L(5)/S(1) motion segment with percutaneous axial lumbosacral screw and analyze the biomechanical stress on the screw.

Methods: With the help of related software and the Mechanical Virtual Human of China, a three-dimensional finite element model was established. Three different loading conditions on the screw were analyzed with this model.

[Altered expression of Ank protein in vertebral end plate].

Objective: To observe the expression change of ANK protein in normal and degenerative vertebral endplate chondrocytes and explore the correlation between ANK gene expression and intervertebral disc degeneration.

Methods: Cartilaginous endplates of 45 patients were divided into experiment group (28 with cervical spondylotic myelopathy including 17 males and 11 females) and control group (17 with fracture or dislocation of cervical spine including 10 males and 7 females). The MRI examinations revealed that all the endplate in control group were grade 0 and grade I-III in experiment group according to Miller's classification.

[Finite element analysis of screw in percutaneous axial lumbosacral interbody fusion].

Objective: To establish a 3D finite element model of L5/S1 motion segment with percutaneous axial lumbosacral interbody fusion (AxiaLIF) screw and conduct a preliminary analysis of biomechanical stress.

Methods: The titanium screw was implanted in L5 and S1 vertebral body. Solid model was established by CAD software according to the actual dimensions of screw.

[Establishment of an in vitro model of natural degeneration of lumbar endplate chondrocytes and expression of Sox9 therein].

Objective: To establish an in vitro model of natural degeneration of lumbar endplate chondrocytes and explore the role and the expression change of Sox9 gene, an important gene in the differentiation and maturation of chondrocyte, in the process of natural degeneration of endplate chondrocytes.

Methods: The lumbar vertebrae of 35 SD rats were taken out to obtain the endplates. Endplate chondrocytes were isolated by enzyme digestion and cultured so as to establish an in vitro natural degeneration model of chondrocytes.