Production of L-Malic Acid by Metabolically Engineered Based on Efficient CRISPR-Cas9 and Cre- Systems.

has been more extensively characterized than other species considering its morphology, physiology, metabolic pathways, and genetic regulation. As it has a rapid growth rate accompanied by simple nutritional requirements and a high tolerance to extreme cultural conditions, is a promising microbial cell factory to biosynthesize various products in industry. However, it remains unclear for whether it is also a suitable host for synthesizing abundant L-malic acid.

Regulation of miR319b-Targeted during the Tomato Response to Low-Potassium Stress.

Potassium deficiency confines root growth and decreases root-to-shoot ratio, thereby limiting root K acquisition. This study aimed to identify the regulation network of microRNA319 involved in low-K stress tolerance in tomato (). SlmiR319b-OE roots demonstrated a smaller root system, a lower number of root hairs and lower K content under low-K stress.

Forecasting seasonal variations in electricity consumption and electricity usage efficiency of industrial sectors using a grey modeling approach.

The aim of this research is to forecast seasonal fluctuations in electricity consumption, and electricity usage efficiency of industrial sectors and identify the impacts of the novel coronavirus disease 2019 (COVID-19). For this purpose, a new seasonal grey prediction model (AWBO-DGGM(1,1)) is proposed: it combines buffer operators and the DGGM(1,1) model. Based on the quarterly data of the industrial enterprises in Zhejiang Province of China from the first quarter of 2013 to the first quarter of 2020, the GM(1,1), DGGM(1,1), SVM, and AWBO-DGGM(1,1) models are employed, respectively, to simulate and forecast seasonal variations in electricity consumption, the added value, and electricity usage efficiency.

An optimised grey buffer operator for forecasting the production and sales of new energy vehicles in China.

In recent years, under a series of policy shocks, the production and sales of new energy vehicles in China show the characteristics of trend mutation and non-smoothness. In order to forecast the production and sales of new energy vehicles in China, an optimised grey buffer operator is proposed by introducing accumulation and translation transformations. Meanwhile, a genetic algorithm is employed to ascertain its optimum parameters.

The NLS-Based Nonlinear Grey Multivariate Model for Forecasting Pollutant Emissions in China.

The relationship between pollutant discharge and economic growth has been a major research focus in environmental economics. To accurately estimate the nonlinear change law of China's pollutant discharge with economic growth, this study establishes a transformed nonlinear grey multivariable (TNGM (1, )) model based on the nonlinear least square (NLS) method. The Gauss-Seidel iterative algorithm was used to solve the parameters of the TNGM (1, ) model based on the NLS basic principle.

Investigation of a Brownfield Conflict Considering the Strength of Preferences.

By employing the Graph Model for Conflict Resolution methodology, this paper models and analyzes a brownfield conflict that occurred at the Changzhou Foreign Language School in Jiangsu, China, in 2016. This conflict made national headlines when news reports revealed that a large number of students and staff suffered from health issues after the school moved to a new site that is built on recently restored land adjacent to the original "Chang Long Chemical" block. Since stakeholders in the conflict hold different strengths of preference, a new option prioritization technique is employed to elicit both crisp preferences and the strength of preferences for the decision-makers (DMs) in the conflict.

Design, synthesis, and biological evaluation of NAD(P)H: Quinone oxidoreductase (NQO1)-targeted oridonin prodrugs possessing indolequinone moiety for hypoxia-selective activation.

The enzyme NQO1 is a potential target for selective cancer therapy due to its overexpression in certain hypoxic tumors. A series of prodrugs possessing a variety of cytotoxic diterpenoids (oridonin and its analogues) as the leaving groups activated by NQO1 were synthesized by functionalization of 3-(hydroxymethyl)indolequinone, which is a good substrate of NQO1. The target compounds (29a-m) exhibited relatively higher antiproliferative activities against NQO1-rich human colon carcinoma cells (HT-29) and human lung carcinoma (A549) cells (IC = 0.

Novel anticancer oridonin derivatives possessing a diazen-1-ium-1,2-diolate nitric oxide donor moiety: Design, synthesis, biological evaluation and nitric oxide release studies.

Oridonin (1) is a complex ent-kaurane diterpenoid with unique antitumor profile, which is isolated from Isodon rubescens. In order to develop novel derivatives of oridonin with improved potency, a series of nitric oxide (NO)-releasing oridonin derivatives were synthesized by coupling diazeniumdiolates with oridonin and its semisynthesized analogues. Their in vitro antiproliferative activity, nitric oxide release ability, and preliminary anticancer mechanism were further evaluated.

Synthesis and antimycobacterial evaluation of natural oridonin and its enmein-type derivatives.

A series of enmein-type derivatives were synthesized and assayed for their antimycobacterial effects. The structures of the synthesized compounds were established by (1)H NMR, (13)C NMR and mass spectral analysis. All the compounds were screened for their antimycobacterial properties against Mycobacterium phlei, Mycobacterium smegmatis and Mycobacterium marinum.

Novel hybrids of natural oridonin-bearing nitrogen mustards as potential anticancer drug candidates.

A series of novel hybrids from natural product oridonin and nitrogen mustards were designed and synthesized to obtain more efficacious and less toxic antitumor agents. The antiproliferative evaluation showed that most conjugates were more potent than their parent compounds oridonin and clinically used nitrogen mustards against four human cancer cell lines (K562, MCF-7, Bel-7402, and MGC-803). Furthermore, the representative compounds 16a-c exhibited antiproliferative activities against the multidrug resistant cell lines (SW620/AD300 and NCI-H460/MX20).

Design, synthesis and antimycobacterial activity evaluation of natural oridonin derivatives.

In an effort to develop novel potent antitubercular drugs, thirty-one oridonin derivatives were designed and prepared. All the compounds obtained were screened for their in vitro activities against Mycobacterium phlei, Mycobacterium smegmatis and Mycobacterium marinum. Among them, thirteen compounds showed significant inhibitory activity against M.

Enmein-type diterpenoid analogs from natural kaurene-type oridonin: Synthesis and their antitumor biological evaluation.

A series of enmein-type diterpenoid analogs (11-20) derived from natural kaurene-type diterpenoid oridonin were synthesized and biologically evaluated. All target compounds showed improved anti-proliferative activities against four human cancer cell lines compared with natural oridonin and parent compound 10. Some compounds were more potent than positive control Taxol.

Library construction and biological evaluation of enmein-type diterpenoid analogues as potential anticancer agents.

A library of promising enmein-type 14-O-diterpenoid derivatives was constructed from a commercially available kaurene-type oridonin by practical and efficient synthetic methods. These synthetic derivatives were evaluated for their antiproliferative activities against a set of four human cancer cell lines. The IC50 values are similar to or improved over those of the parent molecule and paclitaxel, the latter of which was used as a positive control.