Cinobufacini injection (CI), an aqueous extract of , is clinically used for cancer therapy in China, but its molecular mechanism for the treatment of osteosarcoma (OS) remains unclear. We constructed U2OS ectopic subcutaneous tumor model to verify the anti-OS effect of CI . Meanwhile, cell proliferation of U2OS and MG63 cells was monitored using the CCK-8 assay, colony formation and morphological changes.
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