[Effects of mid-palatal suture opening and midfacial bony structure changes induced by maxillary skeletal expansion in adults].

Purpose: To analyze the opening of mid-palatal suture, transverse changes in dental and dentoalveolar measurements and shift of midfacial bony structures induced by maxillary skeletal expansion (MSE) with cone-beam CT (CBCT), and to evaluate the effect of maxillary skeletal expansion and its influence on adjacent bony structures in adults.

Methods: The study sample consisted of 12 adult patients with maxillary transverse deficiency (4 males, 8 females) at a mean age of (21.17±4.

[Generation and validation of inducible osteoblast-specific Stat3 knockout mice].

Purpose: Based on the Cre-Loxp gene knockout system, this study intended to construct tamoxifen-inducible STAT3 conditional knockout mice and verify the knockout efficiency.

Methods: The inducible osteoblasts-specific Stat3 knockout mice Stat3Col1ERT2 were obtained by hybridization through C57 mice of Stat3fl/fl and Col1 creERT2. Bone mesenchymal stem cells(BMSCs) of these mice were isolated and cultured with or without 4-hydroxytamoxin(4-OTH), to verify the effect of Stat3 knockout in vitro by real-time quantitative PCR and Western blotting in the level of mRNA and protein.

[Expression of STAT3 in alveolar bone during orthodontic tooth movement in rats].

Purpose: To study the changes of expression of signal transducer and activator of transcription 3 (STAT3) during orthodontic tooth movement (OTM).

Methods: Twenty six-week-old SD rats were selected, the upper left first molars were moved by coil spring and lasted for 7 days. The maxillary tissues were obtained at 0, 1, 3, 7 d and subjected to histological study.

[The effect of estrogen on adipogenic differentiation of rat bone mesenchymal stem cells].

Purpose: In this study, 10⁻⁹ mol/L 17 β-estradiol (E2) was applied in the adipogenic differentiation of rat bone mesenchymal stem cells (rBMSCs) and the effect of E2 was explored.

Methods: Rat BMSCs were obtained from the femurs and tibias of SD rats. 10⁻⁹ mol/L E2 was involved in the adipogenic differentiation of rBMSCs.

[The effect of estrogen on proliferation and osteogenic differentiation of rat bone marrow mesenchymal stem cells].

Purpose: Different concentrations of 17 β-estradiol (E2) were applied in the osteogenic differentiation of rat bone mesenchymal stem cells (BMSCs), and the proliferation and apoptosis of BMSCs were explored.

Methods: BMSCs were obtained from the femurs and tibias of SD rats. The proliferation curve was conducted to rBMSCs in culture medium containing 0, 10(-9), and 10(-7) mol/L 17 β-estradiol by CCK-8 for 7 days.

[Changes of the microarchitecture of alveolar bone due to different duration of ovariectomy: a Micro-CT study in rats].

Purpose: To study the changes of microarchitecture of alveolar bone due to different duration of ovariectomy in rats.

Methods: Twenty-four virgin Sprague-Dawley rats were randomly assigned to ovariectomy group (OVX) or sham-ovariectomy group (sham). OVX rats were subjected to bilateral ovariectomy and sham-ovariectomy was conducted in sham rats.

[Osteogenic gene expression of human periodontal ligament stem cells during osteogenic induction].

Urpose: To isolate and identify the human periodontal ligament stem cells, evaluate osteogenetic capacity, and investigate the changes of osteogenic bone related gene expression in mineralized medium at different times.

Methods: PDLSCs were isolated by tissue culture and magnetic activated cell sorting. Immunofluorescence staining was used for identification.

[Exploration for micro-osteotomy assisted orthodontic treatment of skeletal Class III malocclusions with alveolar hypoplasia in the lower anterior region].

Purpose: To investigate the changes of periodontal conditions after micro-osteotomy assisted lower incisor decompensation for skeletal Class III malocclusions with alveolar hypoplasia in the lower anterior region.

Methods: The sample consisted of 22 cases diagnosed as skeletal Class III malocclusions with alveolar hypoplasia in the lower anterior region, selected from consecutive patients of Department of Oral & Cranio-maxillofacial Science of Shanghai Ninth People's Hospital during 2009-2012. The samples were divided into 2 groups; G1 comprised 10 patients who accepted micro-osteotomy assisted lower incisor decompensation; G2 comprised 12 patients who chose traditional pre-surgical decomposition.

[Effects of osteoporosis on experimental tooth movement in aged rats].

Objective: To examine the experimental tooth movement and recruitment of new osteoclasts in aged variotomized (OVX) rats.

Methods: Eighty-two 3-month female virgin health rats were divided into two groups. Each group was divided into seven sub-groups of tooth movement: 0,1,3,5,7,10 and 14 days.

[Effects of mechanical tensile stress on the expression of ICAM-1 mRNA in osteoblasts differentiated from rBMSCs].

Objective: To investigate the correlation between mechanical tensile stress and the expression of ICAM-1 mRNA in osteoblasts differentiatd from rBMSCs and elucidate the mechanism for osteoclastogenesis regulated by osteoblasts in bone modeling and remodeling during the process of orthodontic tooth movement.

Methods: rBMSCs-derived osteoblasts were isolated and cultured in vitro, and were subjected to static mechanical tensile stress of 1,3,5 kPa or dynamic tensile stress of 3, 5 kPa at 0.017 Hz with the use of cellular tension-stress system for 24 h.