[Intramuscular type of nodular fasciitis: a report of 3 cases and review of literature].

Purpose: To retrospectively analyze the clinical characteristics of impacted supernumerary teeth in 115 patients.

Methods: One hundred and fifteen patients with im-pacted supernumerary teeth who were admitted to the Department of Oral and Max-illofacial Surgery of Hefei Stomatological Hospital were selected randomly. The age, sex, number of teeth, location, direction, clinical manifestation, anaes-thesia method and operation time were analyzed retrospectively, T test and Chi-square test were used to determine the statistical differences with SPSS 19.

[Synovial chondromatosis in the temporomandibular joint: report of 2 cases].

The clinical manifestations, radiographic findings, intraoperative view, histopathologic features of synovial chondromatosis（SC） in the temporomandibular joint (TMJ) were summarized in 2 cases. Preoperative symptoms included preauricular pain（2/2）, swelling（2/2） and limitation of mouth opening (0/2). X-ray findings showed widened joint space.

[Pigmented villonodular synovitis of the temporomandibular joint：report of one case and review of literatures].

Pigmented villonodular synovitis (PVNS) is a benign， proliferative disorder of synovium. It often affects the knee, and rarely occurs in the temporomandibular joint (TMJ). This paper reported a 45-year-old male patient with PVNS of the TMJ, who was referred with a chief complaint of slowly growing and painless preauricular mass that was noticed about 1 year.

[Soft-tissue osteomas in buccal space: a case report and review of the literatures].

Osteoma is a benign tumor, which is composed of mature differentiated bone tissue .Osteoma can be central, peripheral or extraskeletal. Extraskeletal osteoma also is called soft-tissue osteoma.

[The effect evaluation of suction drainage to prevent fistula after superficial parotidectomy].

Purpose: To evaluate the clinical value of suction drainage to prevent fistula after parotidectomy, and seek the best opportunity to remove the drainage according to the draining output and duration.

Methods: One hundred and ninety-four patients with parotid diseases after superficial parotidectomy were assigned into pressure dressing group and suction drainage group. Pressure dressing was used after suction drainage tube was removed in the pressure group, while suction drainage tube was fixed through the process in the suction group.

Gene mutations in the D-loop region of mitochondrial DNA in oral squamous cell carcinoma.

The present study aimed to investigate gene mutations in the displacement‑loop (D‑loop) region of mitochondrial DNA (mtDNA) in patients with oral squamous cell carcinoma (OSCC) in order to examine the role of gene mutation in mtDNA in OSCC tumorigenesis. mtDNA was obtained from cancer tissues, paracancerous tissues and normal mucosal tissues of thirty patients with OSCC. The D‑loop region of the mtDNA was amplified using polymerase chain reaction, sequenced and then analyzed by Chromas software and BLAST to identify the mutation sites.

[Screening of the gene mutation in D-loop region of mitochondrial DNA in oral squamous cell carcinoma].

Objective: To investigate the gene mutation in D-loop region of mitochondrial DNA (mtDNA) in oral squamous cell carcinoma (OSCC) tissue and to explore the role of the gene mutation in D-loop region in the OSCC tumorigenesis.

Methods: mtDNA was obtained from cancer, paracancerous and normal mucosa tissues of thirty patients with OSCC. The D-loop regions of mtDNA were amplified with PCR, sequencing and then analyzed by Chromas software and BLAST to identify the mutation site.

A preliminary study on the application of bone marrow stromal cell sheet on the formation of functional tissue-engineered bone in dogs.

Purpose: The aim of this study was to construct functional tissue-engineered bone in dogs using cell sheet engineering, a new technique to gain and transfer seed cells.

Materials And Methods: Demineralized bone matrixes, prepared from homologous bone, were coated with recombinant human bone morphogenetic protein-2. Bone marrow stromal cells (BMSCs) were isolated and subcultured.

Single nucleotide polymorphisms in the u-PA gene are related to susceptibility to oral tongue squamous cell carcinoma in the Northern Chinese Han population.

Aim: The purpose of this study was to determine whether susceptibility to oral tongue squamous cell carcinoma (OSCC) is related to polymorphisms in the u-PA gene.

Methods: We examined the rs2227564 C/T and rs2227562 G/A single nucleotide polymorphisms (SNPs) in 196 OSCC patients and 201 age- and gender- matched controls via direct sequencing and PCR-RFLP methods.

Results: Significant differences were found in allelic and genotypic distributions of the rs2227564 and rs2227562 loci when comparing cases and controls.

[Construction of tissue engineering bone with bone marrow stromal cell sheets].

Objective: To construct tissue engineering bone with bone marrow stromal cell (BMSC) sheets of dogs.

Methods: BMSC were derived from dog bone marrow and cell sheets were prepared with temperature-responsive dishes after the cells were induced by osteogenesis. Allogeneic dogs decalcification bone matrixes (DBM) were prepared.

[The mutations of germline succinate dehydrogrnase subunit B (SDHB) in sporadic paragangliomas].

Purpose: The purpose of this study was to investigate the somatic mutations of human mitochondria succinate dehydrogenase subunit B (SDHB) in sporadic paragangliomas.

Methods: Eight exons of SDHB gene in 8 sporadic paragangliomas cases were amplified by PCR and sequenced, respectively. The sequences were analyzed to find mutations compared with human homology sequence in Genebank and SNP database.

[An experimental study of endothelial progenitor cells on bone formation in tissue-engineered bone in dogs].

Purpose: To observe the effects of endothelial progenitor cells (EPCs) on bone formation of tissue engineered bone in dogs.

Methods: Bone marrow stromal cells (BMSCs) and EPCs were derived from dog bone marrow and cultured in different medium in vitro. They were seeded on demineralized bone matrix (DBM) to build tissue engineered bone, then the construct was implanted into the fasciae of latissimus dorsi muscle, the degree of bone formation was analyzed with imaging and histological methods at different time points.

[Clinical observation of acupuncture and massage therapy for temporomandibular joint disorders].

Purpose: To explore simple and effective treatment method for temporomandibular joint disorders by investigating 96 temporomandibular joint disorder patients using electroacupuncture pulse stimulation and massage therapy.

Methods: Ninety-six patients with temporomandibular joint disorder including 54 patients with myofacial pain and 42 patients with external pterygoid muscle spasm, were invloved and then divided into electroacupuncture group and massage combined with electroacupuncture group equally and randomly, with 48 cases in each group. Patients in the electroacupuncture group only received electroacupuncture pulse stimulation therapy alone at the acupoints in the affected sides, including Shangguan, Xiaguan, Jiache, Tinggong and Hegu acupoint.

[The mutations of the D-loop hypervariable region II and hypervariable region III of mitochondrial DNA in oral squamous cell carcinoma].

Objective: To investigate the frequency of mitochondrial DNA (mtDNA) D-loop hypervariable region II (HVR II) and hypervariable region III (HVR III) mutations in oral squamous cell carcinoma (OSCC) and their correlation to provide the new targets for the prevention and treatment of OSCC.

Methods: The D-loop HVR II and HVR III regions of mtDNA in seven cases with OSCC tissues, matched with paracancerous tissues and normal mucosa tissues from the same case, were amplified by polymerase chain raction (PCR), then were detected by direct sequencing to find the mutantsites after the comparison of all sequencing results with the mtDNA Cambridge sequence in the GenBank database.

Results: 82 (56 species) nucleotide changes, with 51(26 species) nucleotide polymorphism, were found after the comparison of all sequencing results with the mtDNA Cambridge sequence in the GenBank database.

[Measurement of mandible bone mineral density of adults in north China].

Objective: To measure the bone mineral density (BMD) of mandible and investigate the relationship between mandible and the whole body skeleton BMD.

Methods: Healthy volunteers were recruited in north China, which were divided into 6 groups by age: > or = 20, > or = 30, > or = 40, > or = 50, > or = 60 and > or = 70 years older, 10 male and 10 female in each group. Dual-energy X-ray absoptiometery (DXA) was used to measure the BMD of the lumbar spine, the mentum of mandible and the mandibular angle.

[A comparison between CT and SPECT in detecting mandibular invasion by lower gingival squamous cell carcinoma].

Purpose: The purpose of this study is to compare the value of CT and SPECT in diagnosis of lower gingival carcinoma invading the mandible.

Methods: From February 2002 to October 2006, twenty-one patients with lower gingival squamous cell carcinoma were enrolled.The data of CT and SPECT were studied,and compared with histopathological findings.

[Maxillary sinus lifting and simultaneously placement of implants from the top of alveoli].

Objective: To study the effect of maxillary sinus floor elevation by the Frialit-2 Bone Condenser for implantation.

Methods: 11 patients underwent sinus floor lift by The Frialit-2 Bone Condenser and were inserted 14 implants. The time of following up was 10 - 21 months.