S-Nitrosylation of Akt by organic nitrate delays revascularization and the recovery of cardiac function in mice following myocardial infarction.

The effects of long-term nitrate therapy are compromised due to protein S-Nitrosylation, which is mediated by nitric oxide (NO). This study is to determine the role of Akt S-Nitrosylation in the recovery of heart functions after ischaemia. In recombinant Akt protein and in HEK293 cells, NO donor decreased Akt activity and induced Akt S-Nitrosylation, but was abolished if Akt protein was mutated by replacing cysteine 296/344 with alanine (Akt-C296/344A).

A systematic proteomic analysis of NaCl-stressed germinating maize seeds.

Salt (NaCl) is a common physiological stressor of plants. To better understand how germinating seeds respond to salt stress, we examined the changes that occurred in the proteome of maize seeds during NaCl-treated germination. Phenotypically, salt concentrations less than 0.

Morphologic characteristics of late stent malapposition after drug-eluting stents implantation by optical coherence tomography follow-up.

Background: Late stent malapposition was frequently observed after DES implantation, which has been associated with the occurrence of late stent thrombosis due to poor neointimal coverage. This study was designed to evaluate the frequency of late stent malapposition at least 1 year after different DESs implantation by optical coherence tomography (OCT).

Methods: Angiographic and OCT examinations were given to 68 patients who had received total 126 various DESs implantation for at least 1 year to detect late stent malapposition.

[Analysis of cDNA library of Cucumis sativus L. challenged by Pseudomonas syringae pv. Lachrymans].

A cDNA library was constructed from the leaves of the disease-resistant cucumber (Cucumis sativus L.) cultivar 'D0462' challenged by Pseudomonas syringae pv. Lachrymans for 48 h.

Diaqua-bis[5-(2-pyrid-yl)-1H-tetra-zolato-κN,N]cobalt(II).

In the title compound, [Co(C(6)H(4)N(5))(2)(H(2)O)(2)], the Co atom is bonded to two water mol-ecules and two bidentate 5-(2-pyrid-yl)tetra-zolate ligands resulting in a slightly distorted octa-hedral CoN(4)O(2) coordination geometry. The Co(II) cation is situated on a crystallographic center of inversion. The asymmetric unit therefore comprises one-half of the mol-ecule.

[Combined use of optical coherence tomography and intravascular ultrasound during percutaneous coronary intervention in patients with coronary artery disease].

Objective: To evaluate the value of combined optical coherence tomography (OCT) and intravascular ultrasound (IVUS) examinations in detecting coronary artery plaque during percutaneous transluminal coronary intervention (PCI).

Methods: OCT and IVUS examinations were performed on 30 diseased coronary vessels from 27 patients underwent PCI from Feb. 2008 to July.

A shotgun phosphoproteomics analysis of embryos in germinated maize seeds.

To better understand the role that reversible protein phosphorylation plays in seed germination, we initiated a phosphoproteomic investigation of embryos of germinated maize seeds. A total of 776 proteins including 39 kinases, 16 phosphatases, and 33 phosphoproteins containing 36 precise in vivo phosphorylation sites were identified. All the phosphorylation sites identified, with the exception of the phosphorylation site on HSP22, have not been reported previously (Lund et al.

Diaqua-1κO,3κO-di-μ-cyanido-1:2κN:C;2:3κC:N-dicyanido-2κN-bis-{4,4'-dibromo-2,2'-[propane-1,2-diylbis(nitrilo-methyl-idyne)]diphenolato}-1κO,N,N',O';3κO,N,N',O'-1,3-dimanganese(III)-2-nickel(II).

In the title compound, [Mn(2)Ni(C(17)H(14)Br(2)N(2)O(2))(2)(CN)(4)(H(2)O)(2)] or [{Mn(C(17)H(14)Br(2)N(2)O(2))(H(2)O)}(2)(μ-CN)(2){Ni(CN)(2)}], each Mn(III) atom is chelated by a Schiff base ligand via two N and two O atoms and is additionally coordinated by a water mol-ecule to give a slightly distorted octa-hedral geometry. Two such Mn(III) ions are linked by a square-planar Ni(CN)(4) unit, which lies on an inversion centre. A two-dimensional network is formed by O-H⋯O and O-H⋯N hydrogen bonds.