[Elesclomol-Cu Induces Cuproptosis in Human Acute Myeloid Leukemia Cells].

Objective: To investigate the effects of elesclomol-Cu (ES-Cu) on the proliferation and cuproptosis of human acute myeloid leukemia (AML) cells.

Methods: The effects of ES-Cu on the proliferation of AML cells and the AML cells pre-treated with ammonium tetrathiomolybdate (TTM) were examined by CCK-8 assay. The Calcein/PI kit was used to detected the changes in activity and cytotoxicity of AML cells induced by ES-Cu.

Development and validation of a rapid LC-MS/MS method to quantify letrozole in human plasma and its application to therapeutic drug monitoring.

A selective, rapid, and sensitive liquid chromatography-tandem mass spectrometry(LC-MS/MS) method was developed and validated for the determination of letrozole (LTZ) in human plasma, using anastrozole as internal standard (IS). Sample preparation was performed by one-step protein precipitation with methanol. The analyte and IS were chromatographed on a reversed-phase YMC-ODS-C18 column (2.

Pharmacokinetics and bioequivalence study of three oral formulations of ambroxol 30 mg: a randomized, three-period crossover comparison in healthy volunteers.

Objective: To compare the pharmacokinetic properties of two newly developed generic ambroxol formulations with a branded innovator product in healthy Chinese male volunteers.

Methods: This was a single-dose, randomized, open-label, three-period crossover study in healthy volunteers aged 18 - 45 years under fasting conditions. Subjects were assigned to receive 1 of 2 test formulations or a reference tablet of ambroxol 30 mg.

[An approach to predicting antioxidative activities of natural products based on near infrared spectroscopy].

The aim of present study is to develop a new approach to predicting antioxidative activities of flavonoid extracts from traditional Chinese medicine based on near-infrared spectroscopy (NIR). The 1,1-diphenyl-2-picryl-hydrazyl (DPPH) method was employed to assay the antioxidative activities of twenty-eight extracts. Then, the near infrared diffuse reflectance spectra of those samples were acquired in the range of 4000-10,000 cm(-1).