Thiol-ene photoimmobilization of chymotrypsin on polysiloxane gels for enzymatic peptide synthesis.

Chemical incorporation of enzymes onto polymeric materials has recently attracted intense scientific attention. Cross-linked polysiloxane gels as a typical super-hydrophobic support, are a good candidate for supporting enzymes in low-water organic medium to efficiently catalyze peptide synthesis because the hydrophobic polysiloxane matrix can prevent water from attacking the acyl-enzyme intermediate, which is beneficial for the shift in equilibrium to peptide formation. In this work, we develop a facile strategy to photoimmobilize olefin-functionalized chymotrypsin onto cross-linked polysiloxane gels UV-initiated thiol-ene click chemistry.

A calamitic mesogenic near-infrared absorbing croconaine dye/liquid crystalline elastomer composite.

In this work, we report the first example of a calamitic mesogenic near-infrared (NIR) absorbing organic dye, made by functionalizing a thiophene-croconaine chromophore rigid core with two symmetric long flexible alkyl chains. The liquid crystal (LC) NIR dye exhibits a sharp and intense NIR absorption band with a maximum absorption peak at 796 nm. Taking advantage of the improved solubility of dispersed in mesogenic molecules, a homogeneously-aligned mono-domain liquid crystalline elastomer (LCE)/ composite film is successfully prepared by applying the classical LC-cell-alignment method and photo-polymerization of photocurable LC monomer mixtures.

Near-infrared-responsive gold nanorod/liquid crystalline elastomer composites prepared by sequential thiol-click chemistry.

A novel NIR-responsive GNR/LCE composite fiber material was prepared by a three-step sequential thiol-click chemistry approach. Taking advantage of GNRs' significant photo-thermal effect, a GNR/LCE composite material with a very low Au loading-level (0.09 wt%), under 808 nm NIR stimulus achieved the N-to-I transition and shrank dramatically in an ambient environment.

[Evaluation on methodological problems in reports concerning quantitative analysis of syndrome differentiation of diabetes mellitus].

Objective: To evaluate the quality of reports published in recent 10 years in China about quantitative analysis of syndrome differentiation for diabetes mellitus (DM) in order to explore the methodological problems in these reports and find possible solutions.

Methods: The main medical literature databases in China were searched. Thirty-one articles were included and evaluated by the principles of clinical epidemiology.