[Acupoint application of herbal paste relieves symptoms and naso-mucosal inflammatory response by down-regulating serum TGF-β1 in allergic rhinitis rats].

Objective: To observe the effect of acupoint application of herbal paste on symptoms of allergic rhinitis (AR), serum immunoglobulin E (IgE) and transforming growth factor beta 1 (TGF-β1) level, and number of nasal eosinophils (EOS) in rats with AR, so as to explore its underlying mechanisms.

Methods: Forty male Wistar rats were randomly divided into normal control, model, medication and acupoint application groups (＝10 rats per group). The AR model was established by intraperitoneal (i.

Study on Site of Action of Sinapine Thiocyanate following Acupoint Herbal Patching.

Objective: To investigate the site of action of sinapine thiocyanate (ST), following acupoint herbal patching (AHP).

Methods: Twenty Wistar rats were randomized into five groups (groups A, B, C, D, and E), and all groups received the same AHP . Skin samples were excised at 2 h, 4 h, 6 h, 10 h, and 26 h after AHP administration from group A to group E separately and the concentrations of ST in the skin were determined using a liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) method.

Downregulation of human Cdc6 protein using a lentivirus RNA interference expression vector.

Eukaryotic CDC6 gene function is required for the initiation of DNA replication and is a key regulatory protein during cell cycle progression. The human CDC6 gene is not expressed in most normal tissues, in contrast with its marked expression in proliferating cancer cells. An effective way to explore the gene functions of CDC6 is to knock-down the CDC6 messenger RNA (mRNA) and examine the phenotypic consequences.