Validity, reliability, and application of the electronic version of a chronic kidney disease patient awareness questionnaire: a pilot study.

Objectives: A questionnaire which provides desirable reliability and validity has been previously developed to assess the disease awareness of diagnosed chronic kidney disease (CKD) patients. However, conventional paper questionnaires often have disadvantages, including recall bias. To substantially improve this, we therefore aimed to explore the feasibility of developing a smartphone-based electronic version (e-version) based upon its original paper version and subsequently tested its validity, reliability, and applicability.

[Application of purified recombinant outer membrane lipoprotein LipL32 in detecting antibodies among leptospirosis cases].

Objective: To establish recombinant outer membrane lipoprotein LipL32-based antibody detection assays in identifying leptospirosis.

Methods: Recombinant leptospiral outer membrane protein LipL32 was obtained by genetic engineering method. This purified protein was used in the indirect and sandwich ELISA assays to test the antibodies in sera of human beings and rats, and the results were compared with those obtained by microscopy agglutination test (MAT) and imported ELISA kit.

[A sero-epidemiology study on hepatitis E virus infection in Fujian province].

Objective: To understand the sero-prevalence of hepatitis E virus (HEV) infection among different populations and animals in Fujian province.

Methods: One thousand one hundred and fifty-one serum samples were collected from 5 species of animals including swine, dog, cow, sheep and rat. A total of 2209 and 1722 serum samples from the general population and from the exposed population were collected.