Application of a Promising Bone Graft Substitute in Bone Tissue Regeneration: Characterization, Biocompatibility, and Animal Study.

The purpose of the present study was to investigate the effect of local hydroxyapatite (HA) combined with extracted sea cucumber () collagen as a promising bone graft substitute on bone remodeling. Fourier-transform infrared spectroscopy, X-ray diffractometry, transmission electron microscopy, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and Sprague-Dawley rat model were used to characterize the microstructure, cytotoxicity, and bone-healing properties of the investigated biocomposite material. Analytical results found that the hydrothermal reaction-synthesized local HA had a hexagonal close-packed structure.

The potential of the stem cells composite hydrogel wound dressings for promoting wound healing and skin regeneration: In vitro and in vivo evaluation.

In this study, the wound healing properties of the gelatin-based hydrogel (GBH) wound dressing combined with adipose-derived stem cells (ADSCs) were investigated using the mouse and porcine models. The analytical results showed that the ADSCs harvested from the porcine significantly increased cell growth and promoted cell differentiation (adipogenesis and osteogenesis) in comparison to the ADSCs harvested from the mouse in vitro. Moreover, the in vivo results also indicated that the GBH wound dressing combined with ADSCs and its culture medium could potentially accelerate wound healing in the mouse and porcine models.