One-Pot Assay Based on CRISPR/Cas13a Technology for HEV RNA Point-of-Care Testing.

Hepatitis E virus (HEV) poses a serious threat to both public health and animal food safety, thereby highlighting the demands for rapid, sensitive, and easy-to-use detection. This study aimed to develop a One-Pot assay using CRISPR/Cas13a for detecting HEV RNA, suitable for point-of-care testing (POCT) in resource-limited settings. CRISPR/Cas13a combined with reverse transcription polymerase chain reaction (RT-PCR) and reverse transcription recombinase-aided amplification (RT-RAA) was applied to a One-Pot assay device.

High-Performing Direct X-Ray Detection Made of One-Dimensional Perovskite-Like (TMHD)SbBr Single Crystal With Anisotropic Response.

3D and 2D organic hybrid perovskites, as well as double perovskites, have demonstrated their suitability for direct X-ray detection. However, the sensitivity and stability of 3D perovskite X-ray detectors are currently being hindered by the existing constraints on ion movement. Therefore, there is an immediate need to develop X-ray detectors that are both highly sensitive and stable while also being environmentally friendly.

Depressed TFAM promotes acetaminophen-induced hepatotoxicity regulated by DDX3X-PGC1α-NRF2 signaling pathway.

Background: Acetaminophen (APAP)-induced acute liver injury (AILI) is the most prevalent cause of acute liver failure and mitochondrial dysfunction plays a dominant role in the pathogenesis of AILI. Mitochondrial transcription factor A (TFAM) is an important marker for maintaining mitochondrial functional homeostasis, but its functions in AILI are unclear. This study aimed to investigate the function of TFAM and its regulatory molecular mechanism in the progression of AILI.

Comparative analysis of metabolic characteristics and prognostic stratification of HER2-low and HER2-zero breast cancer using F-FDG PET/CT imaging.

Background: Recent advancements in novel anti-human epidermal growth factor receptor 2 (HER2) antibody-drug conjugates (ADCs) have highlighted the emerging HER2-low breast cancer subtype with promising therapeutic efficacy. This study aimed to comparatively analyze the metabolic characteristics and prognostic stratification of HER2-low and HER2-zero breast cancer using baseline fluorine-18 fluorodeoxyglucose (F-FDG) positron emission tomography/computed tomography (PET/CT) imaging.

Methods: Consecutive patients with newly diagnosed breast cancer who underwent F-FDG PET/CT prior to therapy in our hospital were retrospectively reviewed.

Axillary lymph node management strategies in cN + breast cancer patients after neoadjuvant chemotherapy.

With the widespread use of neoadjuvant chemotherapy (NAC), the optimal management strategy for axillary lymph nodes following chemotherapy has become a hot topic of discussion. For patients with clinically positive axillary lymph nodes (cN +) (defined as axillary lymph nodes confirmed positive by pathology before NAC), axillary lymph node dissection (ALND) remains the current standard treatment. However, there is still no consensus on whether sentinel lymph node biopsy (SLNB) and other local axillary treatments following NAC can safely replace ALND to reduce injury and complications.

A Rare Case of High-Grade Fetal Adenocarcinoma of the Lung Demonstrated by 18F-FDG PET/CT.

An 85-year-old non-smoking woman with right back pain for 1 month underwent a chest CT, which showed a mass with a maximum diameter of 12.2 cm in the upper lobe of right lung. The lesion demonstrated heterogeneous uptake on FDG PET scanning.

Sub--benziporphyrin: a subcarbaporphyrinoid and its B complex with an unprecedented planar tridentate 14π-aromatic network.

Sub--benziporphyrins were synthesized by Pd-catalyzed cross-coupling of ,'-diboryl--benzitripyrrane with 9,10-bis(1,1-dibromomethylenyl)anthracene. Reaction of sub--benziporphyrin with PhBCl and triethylamine gave its B-phenyl complex as a tetracoordinate nonaromatic B complex. In contrast, the reaction with BBr and triethylamine furnished a neutral B porphyrinoid with a planar and triangular coordination as the first example, in which the -phenylene unit was partially reduced, allowing for the global 14π-aromatic circuit.

Hydrogel dressing composed of nanoAg@QAC promotes the healing of bacterial infected diabetic wounds.

Diabetes mellitus ranks as the eighth most prevalent cause of mortality and disability worldwide. It is a major challenge for clinics to treat diabetic-infected wounds. The hydrogel (referred to as NanoAg@QAC), which combines the advantages of nanosilver (NanoAg) and quaternary ammonium chitosan (QAC), possesses the characteristics of an ideal wound dressing, including proper mechanical properties, antimicrobial activity, anti-biofilm properties, and cytocompatibility.

A Rare Case of Intraperitoneal Infection by Kodamaea ohmeri.

Background: In April 2024, our hospital confirmed a rare case of intra-abdominal infection by Kodamaea ohmeri. The patient sought medical attention at our hospital after taking painkillers orally for one month, experiencing recurrent abdominal pain for 17 days and worsening for 7 days. In March 2024, the patient received symptomatic treatment with oral analgesics (diclofenac sodium) for arthritis.

Oxidations of (2,2'-Diphenyl)ethylidene-Bridged Porphyrin Dimers.

Oxidation reactions of (2,2'-diphenyl)ethylidene-bridged porphyrin dimers were examined for the synthesis of (2,2'-diphenyl)ethylidene-inserted porphyrin arch tape dimers. These reactions provided, in addition to the target arch tape dimers, unexpected products such as a bicyclo[3.3.

[Advancements in the regulatory effects and mechanisms of the immune metabolite itaconate in diseases].

Itaconate is a pivotal intermediate metabolite in the tricarboxylic acid (TCA) cycle of immune cells. It is produced by decarboxylation of -aconitic acid under the catalysis of aconitate decarboxylase 1 (ACOD1), which is encoded by the immune response gene 1 (IRG1). Itaconate has become a focal point of research on immunometabolism.

Double-layer microencapsulation of ammonium polyphosphate and its enhancement on the hydrophobicity and flame retardancy of cellulose paper.

Cellulose paper is a flammable and hygroscopic material, which limits its application. In this paper, melamine-formaldehyde resin (MF) and silane coupling agents were used to microencapsulate ammonium polyphosphate (Si@MFAPP) in turn and added to the fibers suspension to prepare hydrophobic and flame-retardant cellulose paper. It was found that the surface of the ammonium polyphosphate (APP) was smooth with the water solubility of 0.

Meta-analysis: effects of adenoidectomy/tonsillectomy on pediatric maxillary growth development.

Quantitative analysis of adenoid size plays a pivotal role in experimental research, and imaging examinations are extensively employed for this purpose. This study aims to investigate the impact of adenoidectomy/tonsillectomy on the maxillary growth and respiratory outcomes of children. A comprehensive systematic search was conducted across multiple databases, including PubMed, Web of Science and Scopus, utilizing the following keywords: "gland resection", "tonsillectomy", "mouth breathing", "airway obstruction", "low ventilation", "obstructive sleep apnea (OSA) syndrome" and "dental maxillary growth" in articles published between January 2000 and April 2022.

Perceived protective behavioral changes in Chinese residents post-dynamic zero-COVID policy lifting: a cross-sectional study.

Objective: To investigate how Chinese residents perceived changes in their protective behaviors in the early stage after the lifting of the dynamic zero-COVID policy, and to explore the associations between the overall perceived change and factors such as demographic and health-related information, COVID-19 related perceptions, negative emotions, and coping styles.

Methods: This cross-sectional study involved 798 Chinese residents who completed an online questionnaire between 16 and 25 December 2022. The questionnaire covered demographic and health-related information, COVID-19 related perceptions, negative emotions, coping styles, and perceived changes in protective behaviors.

Exploring the Frontier of Cyclic Dipeptides: A Bioinformatics Approach to Potential Therapeutic Applications in Schizophrenia.

Cyclic dipeptides (CDPs), known for their diverse biological activities, have potential therapeutic applications in mental and behavioral disorders (MBDs), particularly schizophrenia. This study explores the CDPs' therapeutic potential using bibliometric analysis, network pharmacology, molecular docking, and experimental verification, focusing on the interactions with the SIGMA1 receptor. A literature review over three decades utilizing the Web of Science Core Collection (WOSCC) was conducted to identify the emerging trends in CDPs research.

Experiences and needs of older patients with stroke in China involved in rehabilitation decision-making: a qualitative study.

Background: Shared decision-making is recommended for stroke rehabilitation. However, the complexity of the rehabilitation modalities exposes patients to decision-making conflicts, exacerbates their disabilities, and diminishes their quality of life. This study aimed to explore the experiences and needs of older patients with stroke in China during rehabilitation decision-making, providing a reference for developing decision-support strategies.

The ZmCPK39-ZmDi19-ZmPR10 immune module regulates quantitative resistance to multiple foliar diseases in maize.

Gray leaf spot, northern leaf blight and southern leaf blight are three of the most destructive foliar diseases affecting maize (Zea mays L.). Here we identified a gene, ZmCPK39, that encodes a calcium-dependent protein kinase and negatively regulates quantitative resistance to these three diseases.

Single-cell analysis of matrisome-related genes in breast invasive carcinoma: new avenues for molecular subtyping and risk estimation.

Background: The incidence of breast cancer remains high and severely affects human health. However, given the heterogeneity of tumor cells, identifying additional characteristics of breast cancer cells is essential for accurate treatment.

Purpose: This study aimed to analyze the relevant characteristics of matrix genes in breast cancer through the multigroup data of a breast cancer multi-database.

Challenges in Accessing Community-Based Rehabilitation and Long-Term Care for Older Adult Stroke Survivors and Their Caregivers: A Qualitative Study.

Background And Objectives: While extensive research has examined the rehabilitation challenges faced by stroke survivors, there is limited literature addressing the difficulties encountered by older adult stroke survivors and their caregivers in accessing rehabilitation and long-term care within the Chinese community. This study aimed to investigate the specific challenges related to access, quality, and coordination of rehabilitation and long-term care experienced by older adult stroke survivors and their caregivers within the Chinese community.

Methods: A qualitative phenomenological design was employed to explore the lived experiences of stroke survivors and their caregivers within the Chinese community.