Exploring the potential mechanisms of sorafenib resistance in hepatocellular carcinoma cell lines based on RNA sequencing.

Background: Exploring the mechanisms underlying sorafenib resistance that arises in hepatocellular carcinoma (HCC) may provide new treatment perspectives.

Methods: Drug-resistant and drug-sensitive HCC cell lines were constructed from existing HepG2 and Huh7 cell lines, and gene expression profiles were determined. Genes differentially expressed between the resistant and sensitive lines were identified and organized into modules based on weighted gene co-expression network analysis.

cGAS-mediated antibacterial immunotherapy against tuberculosis by macrophage-targeted manganese dioxide nanoagonist.

Tuberculosis (TB), induced by Mycobacterium tuberculosis (Mtb) infection, remains one of the top killers among infectious diseases. The pathogenesis hallmarks for TB are complex immune escape mechanisms of Mtb and low targeting effects of anti-TB drugs. cGAS signaling, which is responsible for triggering host antibacterial immunity against Mtb infection, has shown potentials to serve as targets for anti-TB immunotherapy.

Case report: Lobular capillary hemangioma of the right main bronchus: a rare case with calcified and cystic lymph nodes.

Lobular capillary hemangioma (LCH), typically a benign vasoproliferative lesion of the skin or mucosal surfaces, is exceptionally rare in the trachea. Now, we present the second reported case of LCH found in the right intermediate bronchus, characterized by calcification within the lesion and cystic changes. These distinctive features should alert clinicians to consider LCH in the differential diagnosis of other benign vascular tumors and mediastinal lymphadenopathy, particularly when calcified and cystic lesions are observed.

Ferromagnetic transformation of α-FeO Co doping for efficient water oxidation under magnetic field.

The introduction of a magnetic field has great potential for enhancing oxygen evolution reaction (OER) performance, but it requires the catalyst to be ferromagnetic and there is a lack of understanding of the fundamental mechanisms. Herein, we achieved the transformation of non-ferromagnetic α-FeO into ferromagnetic CoFeO by Co doping. Compared to α-FeO, the OER activity of CoFeO considerably increased under a magnetic field of 3000 Oe and is proportional to the magnetic field strength within a certain range.

[Diagnosis and treatment of special type of laryngeal foreign body with granuloma in children].

This article explores the diagnosis and treatment of a special type of laryngeal foreign body with granuloma in children, providing a clinical reference for standardized diagnosis and treatment. This article retrospectively analyzes one case admitted to our department and five other cases reported in relevant literature involving a special type of laryngeal foreign body with granuloma in children. It summarizes the clinical characteristics of this disease and explores its diagnosis and treatment.

Aging-enhanced autophagy activity promotes fibrotic progression via the TGF-β2/Smad signaling pathway in trabecular meshwork cells-a new insight from POAG.

Introduction: Glaucoma, a leading cause of irreversible blindness, is characterized by optic neuropathy and retinopathy, with primary open-angle glaucoma (POAG) being the most prevalent form. The primary pathogenic mechanism of POAG involves elevated intraocular pressure caused by chronic fibrosis of the trabecular meshwork (TM). Autophagy, a critical process for maintaining cellular homeostasis, has been implicated in fibrosis across various organs.

Proximity-activated guide RNA of CRISPR-Cas12a for programmable diagnostic detection and gene regulation.

The flexibility and programmability of CRISPR-Cas technology have made it one of the most popular tools for biomarker diagnostics and gene regulation. Especially, the CRISPR-Cas12 system has shown exceptional clinical diagnosis and gene editing capabilities. Here, we discovered that although the top loop of the 5' handle of guide RNA can undergo central splitting, deactivating CRISPR-Cas12a, the segments can dramatically restore CRISPR function through nucleic acid self-assembly or interactions with small molecules and aptamers.

A Modified Ligature-Induced Peri-Implantitis Murine Model and RNA Sequencing Analysis Compared With Human Subjects.

Aim: To propose a modified method for establishing a peri-implantitis murine model, assess the gene expression profile and immune cell infiltration of the gingiva and alveolar bone, and evaluate the transcriptomic similarity between patients with peri-implantitis and the corresponding murine model.

Materials And Methods: A ligature-induced peri-implantitis murine model was established using an immediate implant placement approach. RNA sequencing was performed to determine the transcriptomic profiles of peri-implant tissues from mice, patients with peri-implantitis and healthy individuals.

Fast and sensitive multivalent spatial pattern-recognition for circular RNA detection.

While circular RNAs (circRNAs) exhibit lower abundance compared to corresponding linear RNAs, they demonstrate potent biological functions. Nevertheless, challenges arise from the low concentration and distinctive structural features of circRNAs, rendering existing methods operationally intricate and less sensitive. Here, we engineer an intelligent tetrahedral DNA framework (TDF) possessing precise spatial pattern-recognition properties with exceptional sensing speed and sensitivity for circRNAs.

RNA modifications in plant adaptation to abiotic stresses.

Epitranscriptomic chemical modifications of RNAs have emerged as potent regulatory mechanisms in the process of plant stress adaptation. Currently, over 170 distinct chemical modifications have been identified in mRNAs, tRNAs, rRNAs, microRNAs (miRNAs), and long noncoding RNAs (lncRNAs). Genetic and molecular studies have identified the genes responsible for addition and removal of chemical modifications from RNA molecules, which are known as "writers" and "erasers," respectively.

Direct synthesis of PtNi coated with NiB for efficient electrochemical hydrogen evolution from seawater.

The design of a protective electron-rich surface is an ideal route to enhance the performance of catalysts. Due to the higher work function of NiB, facile-directed electron transport occurs from PtNi to NiB to enrich electron density on the surface of the NiB shell. This process increases hydrogen adsorption-desorption kinetics and mitigates Cl corrosion.

Circular RNA SCMH1 suppresses KMO expression to inhibit mitophagy and promote functional recovery following stroke.

Metabolic dysfunction is one of the key pathological events after ischemic stroke. Disruption of cerebral blood flow impairs oxygen and energy substrate delivery, leading to mitochondrial oxidative phosphorylation dysfunction and cellular bioenergetic stress. Investigating the effects of circSCMH1, a brain repair-related circular RNA, on metabolism may identify novel therapeutic targets for stroke treatment.

Carboxymethyl Chitosan as a Reversible Template of Calcium Phosphate for Multifunctional Conservation of Carbonate Stone.

The accelerated deterioration of carbonate stone artifacts under climate change has long been an urgent issue. Inspired by biomineralization, we developed carboxymethyl chitosan-diammonium hydrogen phosphate (CD) composite and investigated the conservation effectiveness of the CD composite compared to diammonium hydrogen phosphate (DAP) on limestone. The morphologies and microstructures were characterized using scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FT-IR).

New TIPARP inhibitor rescues mitochondrial function and brain injury in ischemic stroke.

Ischemic stroke is a high-mortality disease that urgently requires new therapeutic strategies. Insufficient cerebral blood supply can induce poly (ADP-ribose) polymerase (PARP) activation and mitochondrial dysfunction, leading to tissue damage and motor dysfunction. We demonstrate that the expression of TCDD inducible PARP (TIPARP) is elevated in ischemic stroke patients and mice.

A transcriptome data set for comparing skin, muscle and dorsal root ganglion between acute and chronic postsurgical pain rats.

Chronic postsurgical pain (CPSP), with a high prevalence and rising epidemic of opioids crisis, is typically derived from acute postoperative pain. Our knowledge on the forming of chronic pain mostly derives from mechanistic studies of pain processing in the brain and spinal cord circuits, yet most pharmacological interventions targeting CNS came to be unhelpful in preventing CPSP. Revealing the peripheral mechanisms behind the transition from acute to chronic pain after surgery could shine a light on the novel analgesic regimens.

LncRNA SNHG16 Inhibits Intracellular Growth Involving Cathelicidin Pathway, Autophagy, and Effector Cytokines Production.

Long noncoding small nucleolar RNA (LncRNA) host gene 16 (SNHG16) is associated with certain diseases, including cancers. However, its role and mechanism in () infection remain unclear. Here, we demonstrated that SNHG16 expression levels were suppressed in peripheral blood mononuclear cells (PBMCs) and CD14 monocytes of tuberculosis (TB) patients.

Membrane Associated RNA-Containing Vesicles Regulate Cortical Astrocytic Microdomain Calcium Transients in Awake Ischemic Stroke Mice.

Astrocytic processes minutely regulate neuronal activity via tripartite synaptic structures. The precision-tuning of the function of astrocytic processes is garnering increasing attention because of its significance in promoting brain repair following ischemic stroke. Microdomain calcium (Ca) transients in astrocytic processes are pivotal for the functional regulation of these processes.

Fever and reversible laboratory abnormalities associated with prolonged use of piperacillin/tazobactam: A case report.

Piperacillin/tazobactam is a widely used anti-infective agent. However, prolonged use can lead to adverse drug reactions, primarily presenting as fever and various abnormal laboratory test results. Certain abnormal test outcomes may mislead clinical assessments.

MIR4435-2HG: A novel biomarker for triple-negative breast cancer diagnosis and prognosis, activating cancer-associated fibroblasts and driving tumor invasion through EMT associated with JNK/c-Jun and p38 MAPK signaling pathway activation.

Background: Breast cancer has the highest incidence rate and causes the most fatalities among all female cancers worldwide. Triple-negative breast cancer (TNBC) is known for its strong invasiveness and higher rates of recurrence. In this research, we aimed to identify MIR4435-2HG as a promising long non-coding RNA (lncRNA) biomarker and therapeutic target for TNBC.

Intranasal Delivery of circATF7IP siRNA via Lipid Nanoparticles Alleviates LPS-induced Depressive-Like Behaviors.

Major depressive disorder (MDD) is a prevalent mental disorder that significantly impacts social and psychological function, but no effective medication is currently available. Circular RNAs (circRNAs) have been reported to participate in the pathogenesis of MDD which are envisioned as promising therapeutic targets. However, nonviral-based delivery strategies targeting circRNA against MDD are not thoroughly investigated.

Self-Assembly of Three-Dimensional Hyperbranched Magnetic Composites and Application in High-Turbidity Water Treatment.

In order to improve dispersibility, polymerization characteristics, chemical stability, and magnetic flocculation performance, magnetic FeO is often assembled with multifarious polymers to realize a functionalization process. Herein, a typical three-dimensional configuration of hyperbranched amino acid polymer (HAAP) was employed to assemble it with FeO, in which we obtained three-dimensional hyperbranched magnetic amino acid composites (FeO@HAAP). The characterization of the FeO@HAAP composites was analyzed, for instance, their size, morphology, structure, configuration, chemical composition, charged characteristics, and magnetic properties.