TMEM74, a lysosome and autophagosome protein, regulates autophagy.

Autophagy is an intracellular degradation/recycling process in eukaryotic cells. It contributes to the turnover of cellular components by delivering portions of the cytoplasm and organelles to lysosomes for digestion. The molecular mechanisms of autophagy and vesicle trafficking, especially the biogenesis and turnover of autophagosomes, are poorly understood.

[Effect of anti-midgut-protein-ingredient antibodies of Anopheles stephensi on the oocysts of Plasmodium yoelii].

Objective: To observe the inhibitory effect of the antibodies against midgut-protein-ingredient of Anopheles stephensi on the oocysts of Plasmodium yoelii.

Methods: Female An. stephensi mosquitoes raised in laboratory were dissected and the midguts were collected.

[Cloning, expression and subcellular localization of a novel human gene-RNF122].

Objective: To clone a functionally unknown gene RNF122 and analyze its expression pattern and subcellular localization.

Methods: PCR was used to clone the novel gene-RNF122 from the mixed human tissue cDNA library. Bioinformatics analysis was used to identify structure characteristics of the gene.