Mechanistic analysis of thermal stability in a novel thermophilic polygalacturonase MlPG28B derived from the marine fungus Mucor lusitanicus.

In this study, heterologous MlPG28B expression was obtained by cloning the Mucor lusitanicus gene screened from a marine environment. The enzyme activity of MlPG28B was maximum at 60 °C, 30 % of the enzyme activity was retained after incubation at 100 °C for 30 min, and enzyme activity was still present after 60 min incubation, one of the best thermostable polygalacturonases characterized until now. The high-purity oligosaccharide standards (DP2-DP7) were prepared with polygalacturonic acid as a substrate.

Analyzing world city network by graph convolutional networks.

British scholar Peter Taylor constructed the World City Network by analyzing the office networks of multinational companies, enabling a network perspective on world cities. However, this method has long been hindered by data deficiencies and update delays. In this study, we utilized publicly available, real-time updated data on global routes to construct the World City Network, thereby addressing the issues of data insufficiency and delayed updates in the existing model.

Recent advances in polygalacturonase: Industrial applications and challenges.

This review focuses on the applications of polygalacturonase (PG), one of the most commercially produced enzymes on the biocatalyst market, in the food, beverage, feed, textile, and paper industries. Most PGs are acidic mesophilic enzymes, as shown by a summary of their biochemical properties. However, the acidic PGs discovered to date are insufficiently effective for industrial applications.