Publications by authors named "Line A Andersen"
Article Synopsis
- A comparative study examined various chromatographic resins, including heparin and cation exchangers, analyzing factors like efficiency, binding strength, and capacity.
- The experiments involved four different proteins, showing that pH had a minimal effect on retention for proteins with high isoelectric points, but certain resins had a notable decrease in retention as pH rose.
- Results indicated that cation-exchange resins’ binding strength varied with ionic strength and protein type, with significant dynamic capacity utilized during operations, revealing potential substitutes for heparin resins in specific conditions.*
A comparative study was performed on strong cation-exchangers to investigate the pH dependence, efficiency, binding strength, particle size distribution, static and dynamic capacity, and SEM pictures of chromatographic resins. The resins tested included: SP Sepharose XL, Poros 50 HS, Toyopearl SP 550c, SP Sepharose BB, Source 30S, TSKGel SP-5PW-HR20, and Toyopearl SP 650c. Testing was performed with four different proteins: anti-FVII Mab (IgG), aprotinin, lysozyme, and myoglobin.
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