Engineered PQQ-Glucose Dehydrogenase as a Universal Biosensor Platform.

Biosensors with direct electron output hold promise for nearly seamless integration with portable electronic devices. However, so far, they have been based on naturally occurring enzymes that significantly limit the spectrum of detectable analytes. Here, we present a novel biosensor architecture based on analyte-driven intermolecular recombination and activity reconstitution of a re-engineered component of glucometers: PQQ-glucose dehydrogenase.

Disposable screen printed sensor for the electrochemical detection of methamphetamine in undiluted saliva.

Background: Methamphetamine has an adverse effect on the ability to drive safely. Police need to quickly screen potentially impaired drivers therefore a rapid disposable test for methamphetamine is highly desirable. This is the first proof-of-concept report of a disposable electrochemical test for methamphetamine in undiluted saliva.

Disposable screen printed sensor for the electrochemical detection of delta-9-tetrahydrocannabinol in undiluted saliva.

Background: Cannabis has an adverse effect on the ability to drive safely, therefore a rapid disposable test for Δ(9)-tetrahydrocannabinol (Δ(9)-THC), the psychoactive component of cannabis, is highly desirable for roadside testing.

Results: A screen printed carbon electrode is used for the N-(4-amino-3-methoxyphenyl)-methanesulfonamide mediated detection of Δ(9)-THC in saliva. Mediator placed in an overlayer was galvanostatically oxidized and reacted with Δ(9)-THC to give an electrochemically active adduct which could be detected by chronoamperometric reduction.

Biosensors and bioelectrochemistry.

This review describes recent developments in the field of biosensors and bioelectrochemistry. Nanoparticles have been used to improve sensor performance and to develop biosensors based on new detection principles. Their use has extended into all areas of biosensor and bioelectrochemistry research.