Dorsal Raphe Dopamine Neurons Modulate Arousal and Promote Wakefulness by Salient Stimuli.

Ventral midbrain dopamine (DA) is unambiguously involved in motivation and behavioral arousal, yet the contributions of other DA populations to these processes are poorly understood. Here, we demonstrate that the dorsal raphe nucleus DA neurons are critical modulators of behavioral arousal and sleep-wake patterning. Using simultaneous fiber photometry and polysomnography, we observed time-delineated dorsal raphe nucleus dopaminergic (DRN) activity upon exposure to arousal-evoking salient cues, irrespective of their hedonic valence.

Temporal analysis of reference frames in parietal cortex area 5d during reach planning.

The neural encoding of spatial and postural reference frames in posterior parietal cortex has traditionally been studied during fixed epochs, but the temporal evolution of these representations (or lack thereof) can provide insight into the underlying computations and functions of this region. Here we present single-unit data recorded from two rhesus macaques during a reach planning task. We found that area 5d coded the position of the hand relative to gaze before presentation of the reach target, but switched to coding the target location relative to hand position soon after target presentation.

Coding of the reach vector in parietal area 5d.

Competing models of sensorimotor computation predict different topological constraints in the brain. Some models propose population coding of particular reference frames in anatomically distinct nodes, whereas others require no such dedicated subpopulations and instead predict that regions will simultaneously code in multiple, intermediate, reference frames. Current empirical evidence is conflicting, partly due to difficulties involved in identifying underlying reference frames.

Comparison of the immediate effects of surgical incision on dorsal horn neuronal receptive field size and responses during postnatal development.

Background: Pain behavior in response to skin incision is developmentally regulated, but little is known about the underlying neuronal mechanisms. The authors hypothesize that the spatial activation and intensity of dorsal horn neuron responses to skin incision differ in immature and adult spinal cord.

Methods: Single wide-dynamic-range dorsal horn cell spike activity was recorded for a minimum of 2 h from anesthetized rat pups aged 7 and 28 days.

Postnatal tuning of cutaneous inhibitory receptive fields in the rat.

Spinal nociceptive processing undergoes extensive maturation in the postnatal period. The large excitatory cutaneous receptive fields and sensitivity to mechanical stimulation in the first weeks of life suggest a lack of inhibitory control in developing spinal sensory pathways, which cannot be easily explained at the synaptic level. We hypothesized that developmental changes in dorsal horn inhibition occur at the network level, and tested this by mapping the spatial and modality organization of dorsal horn cell inhibitory receptive fields (RFs) in decerebrate spinal adult and neonatal rats.