Impact of physical activity on monocyte subset CCR2 expression and macrophage polarization following moderate intensity exercise.

Unlabelled: Coronary artery disease (CAD) is an immune-mediated disease in which CCR2 attracts classical, intermediate, and non-classical monocytes to the arterial intima where they differentiate to macrophages. Balance between pro-inflammatory M1 and anti-inflammatory M2 macrophages contributes to CAD prevention. Moderate to vigorous intensity physical activity (MVPA) elicits an immune response and reduces the incidence of CAD, however, the impact of prior MVPA on monocyte subset CCR2 expression and macrophage polarization following acute exercise is unknown.

Characterization of cell-cell junction changes associated with the formation of a strong endothelial barrier.

A principal function of endothelial cells is the formation of a barrier between the blood and tissues.  This barrier arises from the physical connections at cell-cell junctions, which includes cytoskeletal tight junction and adherens junction proteins. Methods that alter barrier function must therefore affect these cell-cell connections.