Enhancing brown dog tick rearing (Rhipicephalus sanguineus): In vivo feeding using mice (Mus musculus) as blood sources.

Ticks vector a large number of significant pathogens, yet remain understudied due to the challenges in laboratory colonization. This study introduces innovative techniques for rearing Rhipicephalus sanguineus, focusing on in vivo tick feeding using ICR mice (Mus musculus) as a blood source. The research, conducted at the Walter Reed Army Institute of Research - Armed Forces Research Institute of Medical Sciences (WRAIR-AFRIMS), outlines the successful utilization of mice to support all stages of tick development.

B-Spline Solution of the Two-Center Dirac Equation in the Electronic Continuum for Relativistic Molecular Photoionization.

In this work, the two-center Dirac equation is solved numerically using an extension of an adapted B-spline basis set method previously implemented in relativistic atomic calculations (Fischer, C. F.; Zatsarinny, O.

Identification of Bacteria and Viruses Associated with Patients with Acute Febrile Illness in Khon Kaen Province, Thailand.

The majority of cases of undifferentiated acute febrile illness (AFI) in the tropics have an undefined etiology. In Thailand, AFI accounts for two-thirds of illnesses reported to the Ministry of Public Health. To characterize the bacterial and viral causes of these AFIs, we conducted molecular pathogen screening and serological analyses in patients who sought treatment in Chum Phae Hospital, Khon Kaen province, during the period from 2015 to 2016.

Anisotropy Parameters for Two-Color Photoionization Phases in Randomly Oriented Molecules: Theory and Experiment in Methane and Deuteromethane.

We present combined theoretical and experimental work investigating the angle-resolved phases of the photoionization process driven by a two-color field consisting of an attosecond pulse train and an infrared pulse in an ensemble of randomly oriented molecules. We derive a general form for the two-color photoelectron (and time-delay) angular distribution valid also in the case of chiral molecules and when relative polarizations of the photons contributing to the attosecond photoelectron interferometer differ. We show a comparison between the experimental data and theoretical predictions in an ensemble of methane and deuteromethane molecules, discussing the effect of nuclear dynamics on the photoionization phases.

Seasonal pattern of questing ticks and prevalence of pathogenic Rickettsia and Anaplasmataceae in Khao Yai national park, Thailand.

Background: Tick-borne diseases (TBD) are considered neglected diseases in Thailand with disease burden likely underestimated. To assess risk for emerging TBD in Thailand, the seasonality of questing tick and pathogen prevalence were studied in Khao Yai National Park, a top tourist destination.

Methods: During 2019, questing ticks around tourist attractions were systematically collected bimonthly and analyzed for Rickettsia and Anaplasmataceae bacterial species by polymerase chain reaction and DNA sequencing.

The effects of human and rhesus macaque blood meal sources on mosquito reproduction and adult survival under laboratory conditions.

Mass rearing of mosquitoes as required to fulfil research studies is a technically challenging endeavor. Blood meal source has been recognized as a key consideration in mass rearing of mosquitoes that affects colony health and fecundity. Four species of laboratory-colonized mosquitoes from the Department of Entomology, US Army Medical Directorate - Armed Forces Research Institute of Medical Sciences (USAMD-AFRIMS); Anopheles cracens, An.

Applying next generation sequencing to detect tick-pathogens in Dermacentor nuttalli, Ixodes persulcatus, and Hyalomma asiaticum collected from Mongolia.

Ticks and tick-borne diseases represent major threats to the public health of the Mongolian population, of which an estimated 26% live a traditional nomadic pastoralist lifestyle that puts them at increased risk for exposure. Ticks were collected by dragging and removal from livestock in Khentii, Selenge, Tuv, and Umnugovi aimags (provinces) during March-May 2020. Using next-generation sequencing (NGS) with confirmatory PCR and DNA sequencing, we sought to characterize the microbial species present in Dermacentor nuttalli (n = 98), Hyalomma asiaticum (n = 38), and Ixodes persulcatus (n = 72) tick pools.

Borrelia miyamotoi a neglected tick-borne relapsing fever spirochete in Thailand.

Borrelia miyamotoi is a relapsing fever spirochete that shares the same vector as Lyme disease causing Borrelia. This epidemiological study of B. miyamotoi was conducted in rodent reservoirs, tick vectors and human populations simultaneously.

Attosecond dynamics of multi-channel single photon ionization.

Photoionization of atoms and molecules is one of the fastest processes in nature. The understanding of the ultrafast temporal dynamics of this process often requires the characterization of the different angular momentum channels over a broad energy range. Using a two-photon interferometry technique based on extreme ultraviolet and infrared ultrashort pulses, we measure the phase and amplitude of the individual angular momentum channels as a function of kinetic energy in the outer-shell photoionization of neon.

Metagenomic profiles of tick pathogens from across Mongolia, using next generation sequencing.

Tick-borne diseases are a major public health concern in Mongolia. Nomadic pastoralists, which make up ~ 26% of Mongolia's population, are at an increased risk of both tick bite exposure and economic loss associated with clinical disease in herds. This study sought to further characterize tick-borne pathogens present in ticks ( = 1,773) sampled in 2019 from 15 of Mongolia's 21 aimags (provinces).

Laboratory studies of oviposition site choice of Toxorhynchites splendens: Role of larval holding-rearing water and predator-prey interactions.

Oviposition preference by Toxorhynchites mosquito species is an important aspect of their biological control potential. When ovipositing, the females deposit individual eggs by hovering above the water, indicating that they may be capable of detecting chemical cues from above the water surface. These chemical cues may help the females to determine whether the water is suitable for their larvae.

The role of hydraulic and geomorphic complexity in predicting invasive carp spawning potential: St. Croix River, Minnesota and Wisconsin, United States.

Since they were first introduced to the United States more than 50 years ago, invasive carp have rapidly colonized rivers of the Mississippi River Basin, with detrimental effects on native aquatic species. Their continued range expansion, and potential for subsequent invasion of the Great Lakes, has led to increased concern for the susceptibility of as-yet uncompromised lotic and lentic systems in the central United States. Because invasive carp eggs and larvae must drift in the river current for the first several days following spawning, numerical drift modeling has emerged as a useful technique for determining whether certain river systems and reaches have the potential to support suspension-to-hatching survival of invasive carp eggs, a critical first step in recruitment.

Erratum: Semirelativistic Schrödinger Equation for Relativistic Laser-Matter Interactions [Phys. Rev. Lett. 121, 253202 (2018)].

This corrects the article DOI: 10.1103/PhysRevLett.121.

Autoionizing Polaritons in Attosecond Atomic Ionization.

Light-induced states and Autler-Townes splitting of laser-coupled states are common features in the photoionization spectra of laser-dressed atoms. The entangled light-matter character of metastable Autler-Townes multiplets, which makes them autoionizing polaritons, however, is still largely unexplored. We employ attosecond transient-absorption spectroscopy in argon to study the formation of polariton multiplets between the 3s^{-1}4p and several light-induced states.

US Department of Defense Support of Civilian Vector Control Operations Following Natural Disasters.

The United States Department of Defense (DoD) employs advanced-degreed entomologists as Preventive Medicine and Public Health Officers in the Army, Navy, and Air Force. While the primary objective of military entomologists is service member health and readiness ("force health protection"), military entomology resources can provide support to civil authorities as directed by the President or Secretary of Defense through Department of Defense Directive 3025.18, Defense Support of Civil Authorities (DSCA).

Potential of Outdoor Ultra-Low-Volume Aerosol and Thermal Fog to Suppress the Dengue Vector, Aedes aegypti, Inside Dwellings.

A field study investigated penetration of outdoor ground ultra-low-volume (ULV) aerosol and thermal fog adulticide applications into a dwelling to control the dengue vector Aedes aegypti (L). Four applications of Kontrol 4-4 (4.6% permethrin active ingredient [AI], 4.

Attosecond electron-spin dynamics in Xe 4d photoionization.

The photoionization of xenon atoms in the 70-100 eV range reveals several fascinating physical phenomena such as a giant resonance induced by the dynamic rearrangement of the electron cloud after photon absorption, an anomalous branching ratio between intermediate Xe states separated by the spin-orbit interaction and multiple Auger decay processes. These phenomena have been studied in the past, using in particular synchrotron radiation, but without access to real-time dynamics. Here, we study the dynamics of Xe 4d photoionization on its natural time scale combining attosecond interferometry and coincidence spectroscopy.

Fano's Propensity Rule in Angle-Resolved Attosecond Pump-Probe Photoionization.

In a seminal article, Fano predicts that absorption of light occurs preferably with increase of angular momentum. We generalize Fano's propensity rule to laser-assisted photoionization, consisting of absorption of an extreme-ultraviolet photon followed by absorption or emission of an infrared photon. The predicted asymmetry between absorption and emission leads to incomplete quantum interference in attosecond photoelectron interferometry.

Comparative Analysis of Mosquito Trap Counts In the Peruvian Amazon: Effect of Trap Type and Other Covariates On Counts and Diversity.

Efficient detection of multiple species of adult mosquitoes in various habitats using effective traps is a crucial 1st step in any disease prevention program. Novel trap types that target tropical vectors of human diseases require field testing in the habitat of the vector-disease system in question. This paper analyzes a series of mosquito trapping studies conducted at Mapacocha, San Juan Bautista District, Loreto, Peru, during August-September 2013 and April-May 2014.

Semirelativistic Schrödinger Equation for Relativistic Laser-Matter Interactions.

A semirelativistic formulation of light-matter interaction is derived using the so called propagation gauge and the relativistic mass shift. We show that relativistic effects induced by a superintense laser field can, to a surprisingly large extent, be accounted for by the Schrödinger equation, provided that we replace the rest mass in the propagation gauge Hamiltonian by the corresponding time-dependent field-dressed mass. The validity of the semirelativistic approach is tested numerically on a hydrogen atom exposed to an intense extreme ultraviolet laser pulse strong enough to accelerate the electron towards relativistic velocities.

Anisotropic photoemission time delays close to a Fano resonance.

Electron correlation and multielectron effects are fundamental interactions that govern many physical and chemical processes in atomic, molecular and solid state systems. The process of autoionization, induced by resonant excitation of electrons into discrete states present in the spectral continuum of atomic and molecular targets, is mediated by electron correlation. Here we investigate the attosecond photoemission dynamics in argon in the 20-40 eV spectral range, in the vicinity of the 3snp autoionizing resonances.

A Small-Scale Investigation Into the Effect of A Larvicidal Oil On Oviposition Site Preference By Aedes aegypti.

Larvicidal oils can be used to control immature Aedes aegypti and other pestiferous mosquitoes. To test whether oil-based larvicides also act as oviposition deterrents, indoor and semi-field trials were conducted at the Navy Entomology Center of Excellence in Jacksonville, FL. In both studies, treatment cages consisted of oviposition cups lined with seed germination paper as an oviposition substrate and filled with 1-wk-old southern live oak ( Quercus virginiana) leaf litter-infused water.

Erratum: Resonance Effects in Photoemission Time Delays [Phys. Rev. Lett. 115, 133001 (2015)].

This corrects the article DOI: 10.1103/PhysRevLett.115.