Investigation of the effects of deracoxib and piroxicam on the in vitro viability of osteosarcoma cells from dogs.

Objective: To determine whether exposure of canine osteosarcoma cells to deracoxib or piroxicam results in decreased viability, whether the cytotoxic effects of deracoxib and piroxicam involve induction of apoptosis, and whether deracoxib is a more potent inhibitor of osteosarcoma cell growth than piroxicam.

Sample Population: 1 fibroblast and 3 osteosarcoma cell lines.

Procedure: Cell counts and viability assays were performed using osteosarcoma cells (POS, highly metastatic POS, and canine osteosarcoma cell 31) and fibroblasts after 72 hours of incubation with deracoxib at concentrations of 0.

Investigation of the effect of pamidronate disodium on the in vitro viability of osteosarcoma cells from dogs.

Objective: To determine the effect of pamidronate disodium on the in vitro viability of osteosarcoma cells and non-neoplastic cells from dogs.

Sample Population: 3 osteosarcoma and 1 fibroblast cell lines derived from dogs.

Procedure: Cell counts and cell viability assays were performed in cultures of osteosarcoma cells (POS, HMPOS, and COS31 cell lines) and fibroblasts after 24, 48, and 72 hours of incubation with pamidronate at concentrations of 0.

The effect of the bisphosphonate alendronate on viability of canine osteosarcoma cells in vitro.

The objective of this study was to determine the effect of alendronate on the viability of canine osteosarcoma cells and nonneoplastic canine cells. The sample population was composed of canine osteosarcoma tumor cells. Osteosarcoma cells and canine fibroblasts were maintained in culture under standard conditions.

The effect of topical tripeptide-copper complex on healing of ischemic open wounds.

Objective: To evaluate the effects of topical glycyl-L-histidyl-L-lysine tripeptide-copper complex (TCC; Iamin 2% Gel; Procyte Corporation, Redmond, WA) on healing in ischemic open wounds.

Study Design: Experimental study.

Sample Population: Twenty-four adult male Sprague-Dawley rats.