Bactericidal and cytotoxic effects of chloramine-T on wound pathogens and human fibroblasts in vitro.

Objectives: To evaluate cytotoxicity and bactericidal effects of chloramine-T.

Methods: In vitro study of various concentrations and exposure times to preparations containing human fibroblasts or 1.5 x 10 colony forming units per milliliter (CFU/mL) of 3 gram-positive bacteria-Staphylococcus aureus, methicillin-resistant S aureus, and vancomycin-resistant Enterococcus faecalis-and 2 gram-negative bacteria-Escherichia coli and Pseudomonas aeruginosa-with and without fetal bovine serum present.