Publications by authors named "Linda Broodtaerts"
Background: Free light chains (FLC) are useful biomarkers for diagnosis and follow-up of plasma cell disorders. FLC quantification is encumbered by non-linearity and antigen excess (>4-fold difference between results obtained at the 2000- and 100-fold dilution).
Methods: FLC concentration was measured with Freelite® reagents on the BNII, using 100- and 2000-fold dilutions in 3645 samples.
Background: Free light chains (FLC) are useful biomarkers in the assessment of plasma cell disorders. Concerns have been raised about some technical aspects of the assay. This report examined the occurrence of dilution anomalies and/or antigen excess.
View Article and Find Full Text PDFDiagnostic accuracy of the FIDIS multiplex fluorescent microsphere immunodetection system for anti-extractable nuclear antigen (ENA) antibodies in connective tissue diseases.
Martine Vercammen Patricia Meirlaen Jacques Sennesael Brigitte Velkeniers Sara T'Kint Linda Broodtaerts
Clin Chem Lab Med
June 2007
Article Synopsis
- Anti-extractable nuclear antigen antibodies (ENA) are important for diagnosing connective tissue diseases (CTDs) and have been examined using different testing methods.
- A comparison of the FIDIS system and traditional methods (INNO-LIA and immunodiffusion) showed no significant differences in sensitivity or specificity for detecting anti-ENA in CTD patients.
- The FIDIS method demonstrated acceptable sensitivity and high specificity, with overall diagnostic performance similar to that of other established testing methods.