The effects of hypothalamic microglial activation on ventricular arrhythmias in stress cardiomyopathy.

Background: Stress cardiomyopathy (SCM) currently has a high incidence in older adults, and the theories regarding its causes include "catecholamine myocardial toxicity" and "sympathetic hyperactivation". However, the role of the central nervous system in the pathogenesis of SCM remains unknown. We investigated the role of microglia activation in the paraventricular hypothalamic nucleus (PVN) in the development of SCM.

Nanoenzyme-based sensors for the detection of anti-tumor drugs.

Natural enzymes are a class of biological catalysts that can catalyze a specific substrate. Although natural enzymes have catalytic activity, they are susceptible to the influence of external environment such as temperature, and storage requirements are more stringent. Since the first discovery of magnetic FeO nanoparticles with peroxidase-like activity in 2007, the research on nanoenzymes has entered a rapid development stage.

Graphite Phase Carbon Nitride Nanosheets-Based Fluorescent Sensors for Analysis and Detection.

Fluorescent sensors reflect information such as the concentration or content of the analysis by interacting with a specific recognition group to change the signal of the fluorophore. It has attracted much attention because of its advantages of high sensitivity, fast detection speed and low cost, and it has become an effective alternative to traditional detection methods. Graphitic phase carbon nitride nanosheets (g-CNNs) are a class of carbon-based fluorescent nanomaterials derived from bulk graphite phase carbon nitride (g-CN), which have attracted much attention from scholars because of their advantages of low cost, simple fabrication, high quantum yield, strong stability and nontoxicity.

Lanthanide coordination polymers as luminescent laccase mimics for ratiometric sensing of dopamine.

Some metal ions, with inner enzyme-like catalytic activity, could be doped into lanthanide coordination polymers (Ln CPs) through coordination, which has been proved as a facile strategy to prepare the luminescent nanozymes. In this study, Cu-doped Ln CPs with laccase-mimic activity and double luminescence were rationally designed and synthesized by self-assembly of guanine monophosphate (GMP), 2-aminoterephthalic acid (ATA), Cu and Tb in buffer solution at room temperature. The obtained probes Tb/Cu-GMP/ATA CPs not only emitted green fluorescence of Tb and blue fluorescence of ATA simultaneously under irradiation at the same wavelength, but also processed enhanced laccase-like activity for catalyzing the oxidation of phenolic substrates.

Genome-wide analysis of WRKY gene family and the dynamic responses of key WRKY genes involved in cadmium stress in .

The WRKY transcription factors comprise one of the most extensive gene families and serve as pivotal regulators of plant responses to heavy metal stress. They contribute significantly to maintaining plant growth and development by enhancing plant tolerance. However, research on the role of WRKY genes in response to cadmium (Cd) stress in mustard is minimal.

A signal-on photoelectrochemical aptasensor based on WO/CdS heterojunction for the ultrasensitive detection of kanamycin.

In this study, a signal-on photoelectrochemical (PEC) aptasensor for the ultrasensitive determination of kanamycin (KANA) was constructed using WO/CdS heterojunction as photoactive material. Firstly, WO/CdS heterojunction with excellent photoelectric response was successfully prepared by simple co-precipitation method, resulting in a strong and stable initial photocurrent. In addition, amino modified aptamers were immobilized on the electrode surface by glutaraldehyde as biological recognition components.

An electrochemical ratiometric immunosensor for the detection of NMP22 based on ZIF-8@MWCNTs@Chit@Fc@AuNPs and AuPt-MB.

Nuclear matrix protein 22 (NMP22) is one of the most important tumor markers of bladder cancer and is significantly elevated in the urine of bladder cancer patients. Therefore, in this work, a highly sensitive ratiometric electrochemical immunosensor was constructed to detect NMP22 based on ZIF-8@MWCNTs@Chit@Fc@AuNPs composites. ZIF-8 had a large surface area and good adsorption ability.

Photocurrent polarity switching photoelectrochemical aptasensor for oxytetracycline based on BiOBr/AgS/PDA//CuO: CuO-induced II-type to dual Z-scheme system.

Background: As a broad-spectrum tetracycline antibiotic, Oxytetracycline (OTC) was widely used in a variety of applications. But, the overuse of OTC had led to the detection of it in food, water and soil, which could present significance risk to human health and cause damage to ecosystem. It was of great significance to develop sensitive detection methods for OTC.

Low-molecular-weight organic acids inhibit the methane-dependent arsenate reduction process in paddy soils.

Anaerobic methane oxidation (AOM) can drive soil arsenate reduction, a process known as methane-dependent arsenate reduction (M-AsR), which is a critical driver of arsenic (As) release in soil. Low molecular weight organic acids (LMWOAs), an important component of rice root exudates, have an unclear influence and mechanism on the M-AsR process. To narrow this knowledge gap, three typical LMWOAs-citric acid, oxalic acid, and acetic acid-were selected and added to As-contaminated paddy soils, followed by the injection of CH and incubation under anaerobic conditions.

Janus liposozyme for the modulation of redox and immune homeostasis in infected diabetic wounds.

Diabetic foot ulcers often become infected, leading to treatment complications and increased risk of loss of limb. Therapeutics to manage infection and simultaneously promote healing are needed. Here we report on the development of a Janus liposozyme that treats infections and promotes wound closure and re-epithelialization.

Lanthanide coordination polymers@CuO nanoparticles: Enhanced self-cascade nanoenzyme activity and ratiometric fluorescence assay of glutathione.

Tandem enzyme can catalyze some cascade reactions with high efficiency, and some few tandem enzyme-like mimics have been discovered recently. Further improving the catalytic efficiency of tandem nanoenzymes with facile method may undoubtedly promote and broaden their applications in various fields. In this work, cupric oxide nanoparticles (CuO NPs) with dual-functional enzyme mimics were synthesized using the rapid deposition method in advance, which simultaneously combined with lanthanide infinite coordination polymers (Ln ICPs) during the self-assemble of Tb, guanine-5'-triphosphate (GTP) and auxiliary ligand terephthalic acid (TA).

Developing Isomeric Peptides for Mimicking the Sequence-Activity Landscapes of Enzyme Evolution.

Enzymes catalyze almost all material conversion processes within living organisms, yet their natural evolution remains unobserved. Short peptides, derived from proteins and featuring active sites, have emerged as promising building blocks for constructing bioactive supramolecular materials that mimic native proteins through self-assembly. Herein, we employ histidine-containing isomeric tetrapeptides KHFF, HKFF, KFHF, HFKF, FKHF, and FHKF to craft supramolecular self-assemblies, aiming to explore the sequence-activity landscapes of enzyme evolution.

Twice-walk strategy based on three-dimensional DNA walking machine driven by duplex-specific nuclease.

A twice-walk strategy based on a three-dimensional (3D) cleat-equipped DNA walking machine with a high signal amplification efficiency was investigated for ultrasensitive detection of miRNA. Impressively, addition of duplex-specific nuclease (DSN) just once drove the twice-walk strategy, making the strategy simpler. With the advantages of being simple, rapid and ultrasensitive, the biosensor offers potential for use in early clinical diagnosis.

Copper-doped Lanthanide Coordination Polymers as Luminescent Nanoenzyme for Ratiometric Sensing of HO and Glutathione.

Design and fabrication of integrated multifunctional probes with intrinsic catalytic and detection abilities is of great importance to simplify the operation in biosensing application with high sensitivity. Herein, dual-emitting lanthanide coordination polymers (Ln-CPs) were facilely prepared by self-assembly of guanine diphosphate (GDP), terephthalic acid (TA), Tb and Cu designated as Tb/Cu-GDP/TA CPs. The doped Cu endowed CPs with obviously enhanced peroxidase mimicking activity compared with free Cu.

Cascade signal amplification strategy for the electrochemical aptasensing of nucleic acid: Combination of dual-output toehold-mediated DNA strand displacement, DNA walker and Exo III.

Background: Sensitive and selective analysis of low content nucleic acid sequences plays an important role in pathogen analysis, disease diagnosis and biomedicine. The electrochemical biosensor based on toehold-mediated strand displacement reaction (TMSD) is highly attractive in nucleic acid detection due to their improved sensitivity and rapid response. But the traditional TMSD carried out on the electrode always with low displacement efficiency and complicated electrode operation, resulting in compromised sensing performance.

Application of hydroxypropyl methylcellulose to improve the wettability of chitosan coating and its preservation performance on tangerine fruits.

Hydroxypropyl methylcellulose (HPMC) was employed as an intermediate layer to enhance interfacial interaction between chitosan (CS) coating and tangerine fruits, thereby improving the preservation effect. Owing to the low surface tension of tangerine fruit (26.04 mN/m), CS coating solutions showed poor wetting properties on fruit peels (contact angle > 100°).

A rapid one-step electrochemical method based on cleat-equipped molecular walking machine.

Various nucleic acid molecular machines have emerged in recent years. However, when the nucleic acid tracks are fully depleted, these walkers are highly susceptible to premature release or stalling in regions where the tracks are locally exhausted. In this work, a molecular walking machine with a cleat domain preventing dissociation from the track was explored for ultrasensitive detection of miRNA.

Histological and functional assessment of a Takotsubo cardiomyopathy model established by immobilization stress.

Introduction: Takotsubo cardiomyopathy (TTC), also known as stress-induced cardiomyopathy, resembles acute heart failure syndrome but lacks disease-specific diagnosis and treatment strategies. TTC accounts for approximately 5-6% of all suspected cases of acute coronary syndrome in women. At present, animal models of TTC are often created by large amounts of exogenous catecholamines such as isoproterenol.

Effects of citric acid on arsenic transformation and microbial communities in different paddy soils.

Root exudate is a major source of soil organic matter and can significantly affect arsenic (As) migration and transformation in paddy soils. Citric acid is the main component of rice root exudate, however, the impacts and rules of citric acid on As bioavailability and rhizobacteria in different soils remains unclear. This study investigated the effects of citric acid on As transformation and microbial community in ten different paddy soils by flooded soil culture experiments.

Comprehensive investigation of BZR gene family in four dicots and the function of PtBZR9 and PtBZR12 under drought stress.

Brassinazole-resistant (BZR) transcription factor plays an important role in plant growth and stress resistance through brassinosteroid (BR) signal transduction. However, systematic analysis of the BZR family in dicots remains limited. In this study, we conducted a genome-wide study of four typical dicots: Arabidopsis thaliana, Carica papaya, Vitis vinifera and Populus trichocarpa.

A signal-on photoelectrochemical aptasensor based on ferrocene labeled triple helix DNA molecular switch for detection of antibiotic amoxicillin.

A sensitive signal-on photoelectrochemical aptasensor for antibiotic determination was constructed based on the energy level matching between ferrocene and CuInS. P-type CuInS microflower was complexed with reduced graphene oxide (CuInS/rGO) to get photocathode current with good photoelectric conversion efficiency and stability. Then, hairpin DNA (HP) was covalently bonded to the electrode surface.

ACS-20/FATP4 mediates the anti-ageing effect of dietary restriction in C. elegans.

Dietary restriction is an effective anti-ageing intervention across species. However, the molecular mechanisms from the metabolic aspects of view are still underexplored. Here we show ACS-20 as a key mediator of dietary restriction on healthy ageing from a genetic screen of the C.

Response and inversion of skewness parameters to meteorological factors based on RGB model of leaf color digital image.

In the natural environment, complex and changeable meteorological factors can influence changes in the internal physiology and phenotype of crops. It is important to learn how to convert complex meteorological factor stimuli into plant perception phenotypes when analyzing the biological data obtained under the natural field condition. We restored the true gradation distribution of leaf color, which is also known as the skewed distribution of color scale, and obtained 20 multi-dimensional color gradation skewness-distribution (CGSD) parameters based on the leaf color skewness parameter system.