High-throughput sequencing explores the genetic variability of endophytic bacteria in three Sichuan bamboo species (, , and ).

Introduction: Bamboo is a sustainable and degradable resource for sustenance, high-strength cellulose microfibers, and synthetic fiber in China. Endophytic bacteria enhance root development and ethylene levels, benefiting the host plants' physiology.

Methods: We investigated the population, diversity, and abundance of endophytic bacteria in the leaves of three bamboo species-, , and -using high-throughput 16S rDNA sequencing.

Engineering of CD8 T cells with an HIV-specific synthetic notch receptor to secrete broadly therapeutic antibodies for combining antiviral humoral and cellular immune responses.

The application of immunotherapeutic strategies, such as chimeric antigen receptor-T cells and broadly neutralizing antibodies (bNAbs), for the treatment of human immunodeficiency virus (HIV) infection is hindered by the latent reservoirs and viral escape. Achieving long-term control of viral load in the absence of antiretroviral therapy requires a combination approach utilizing these immunotherapeutic strategies. For this purpose, we developed novel anti-HIV-1 synthetic Notch (synNotch) receptor-T cells, termed CD4-17b-VN, which express both a bNAb (VRC01) and a bispecific T cell-engaging protein (N6-αCD3) with antigenic control.

Problematic media use, mental health literacy, and their interaction in depression among nursing students.

Background: In the digital age, the relationship between social media use and mental health has gained significant attention. Problematic social media use can negatively affect mental health, while mental health literacy plays a crucial role in mitigating these effects.

Objective: This study aimed to explore the relationships between problematic social media use, mental health literacy, and their interaction with depression among nursing students.

Associations of insulin resistance estimated by glucose disposal rate with frailty progression.

Objectives: To investigate the relationship between estimated glucose disposal rates (eGDR) and the progression of frailty, using longitudinal data.

Methods: We analyzed four waves of data from the China Health and Retirement Longitudinal Study (CHARLS) from 2011 to 2018, involving 6,778 middle-aged and older adults. eGDR was calculated using a specific formula, categorizing participants into high and low eGDR groups based on the lowest quartile (8.

Two rare cases of severe community-acquired bloodstream infections: a clinical case report.

Background: The escalating demographic shift towards an aging population and the widespread occurrence of immunological diseases have contributed to an elevation in the frequency of community-acquired infections. Notably, among these infections, community-acquired bloodstream infections (CABSI) stand out due to their significant lethality. Detailed medical history inquiries, assessment of underlying immune status, detection of the source of infection, and initial precise identification and treatment of the infectious agents can improve the prognosis of CABSI.

Intrinsic capacity and its association with predictors among Chinese empty nest older adults in communities: a latent class analysis.

Objectives: This study aimed to examine Intrinsic Capacity (IC) subgroups and the association of IC subgroups with IC predictors in Chinese urban empty nesters.

Methods: A convenient sample of 385 older adults aged 60 and above in Community Health Service Center was recruited from Hei Longjiang Province, China, between June 2023 and December 2023. Latent class Analysis (LCA) was conducted to explore IC subgroups using the sensory, cognition, locomotion, psychological, and vitality domains of IC as input variables.

Electrochemical Biosensor Based on Dual-Ligand Functionalized Lanthanide-Encapsulated Polyoxometalate Conductive Polymer Film for Detecting Broad-Spectrum Tumor Marker MicroRNA-155.

In this work, a dual-ligand functionalized lanthanide-encapsulated selenotungstate [HN(CH)]NaH[Ho(HO)(HPACA)WO(Ac)][SeWO] · 60HO (, HPACA = 2-pyrazinecarboxylic acid, HAc = acetic acid) was successfully acquired by simultaneously incorporating rigid HPACA and flexible Ac ligands to one reaction system. Interestingly, the polyanion [Ho(HO)(HPACA)WO(Ac)][SeWO] of is composed of six trivacant Keggin-type [B-α-SeWO] units interconnected through an organic-inorganic hybrid dual-ligand bimetallic [Ho(HO)(HPACA)WO(Ac)] cluster. Moreover, the @PNMPy film (PNMPy = poly(-methylpyrrole)) was successfully prepared through an electrochemical polymerization strategy.

Veno-venous extracorporeal membrane oxygenation-assisted treatment of severe airway stenosis due to goiter: Two case reports.

Rationale: Extracorporeal membrane oxygenation (ECMO) is a critical care intervention that acts as a temporary substitute for the heart and lungs, facilitating adequate tissue perfusion and gas exchange. The 2 primary configurations, veno-arterial and veno-venous ECMO, are tailored to support either the heart and lungs or the lungs alone, respectively.

Patient Concerns: The case report details patients with tumor-induced airway stenosis who encountered limitations with standard treatments, which were either insufficient or carried the risk of severe complications such as hypoxia and asphyxia.

Chromosome genome assembly of the (Gamble) with PacBio and Hi-C sequencing data.

Introduction: , a crucial commercial crop and a fundamental component of traditional Chinese medicine, is renowned for its abundant production of volatile terpenoids. However, the lack of available genomic information has hindered pertinent research efforts in the past.

Methods: To bridge this gap, the present study aimed to use PacBio HiFi, short-read, and highthroughput chromosome conformation capture sequencing to construct a chromosome-level assembly of the genome.

Mechanism of PANoptosis in metabolic dysfunction-associated steatotic liver disease.

In recent years, the incidence of metabolic dysfunction-associated steatotic liver disease (MASLD) has been steadily rising, emerging as a major chronic liver disease of global concern. The course of MASLD is varied, spanning from MASLD to metabolic dysfunction associated steatohepatitis (MASH). MASH is an important contributor to cirrhosis, which may subsequently lead to hepatocellular carcinoma.

The impact of wind energy on plant biomass production in China.

Global wind power expansion raises concerns about its potential impact on plant biomass production (PBP). Using a high-dimensional fixed effects model, this study reveals significant PBP reduction due to wind farm construction based on 2404 wind farms, 108,361 wind turbines, and 7,904,352 PBP observations during 2000-2022 in China. Within a 1-10 km buffer, the normalized differential vegetation and enhanced vegetation indices decrease from 0.

Comparative analysis of endophytic fungal communities in bamboo species Phyllostachys edulis, Bambusa rigida, and Pleioblastus amarus.

Fungal endophytes in plant leaf mesophyll form mutually beneficial associations through carbon assimilation, synthesis of biologically active chemicals, and enhancement of aesthetic and nutritional value. Here, we compared community structure, diversity, and richness of endophytic fungi in the leaves of three bamboo species, including Phyllostachys edulis (MZ), Bambusa rigida (KZ), and Pleioblastus amarus (YT) via high-throughput Illumina sequencing. In total, 1070 operational taxonomic units (OTUs) were retrieved and classified into 7 phylum, 27 classes, 82 orders, 185 families, 310 genus, and 448 species.

Cerium-Encapsulated Sb-Se-Templating Polyoxotungstate for Electrochemically Sensing Human Multidrug Resistance Gene Segment.

A tower-like Sb-Se-templating polyoxotungstate [HN(CH)]NaH[Ce/Na(HO)][SbSeWO]·50HO () was synthesized, whose skeleton is assembled from two prolonged lacunary Dawson [SbSeWO] units and two [Ce/Na(HO)] linkers. The uncommon [SbSeWO] unit can be viewed as a combination of one [SeWO] group grafted onto a trivacant Dawson [SbSeWO] subunit. The conductive composite -Au@rGO containing , gold nanoparticles, and reduced graphene oxide (rGO) was conveniently prepared, using which the -Au@rGO-based electrochemical genosensor was constructed for detecting human multidrug resistance gene segment.

Adsorption and Aggregation Behavior in Aqueous Solution of Tetrasiloxane-based Carboxylate Surfactants via "Thiol-ene" Photochemical Reaction.

Anionic silicone surfactants have long been a neglected field. In this paper three anionic silicone surfactants were synthesized first time from dichloromethylvinylsilane through hydrolysis-condensation, "thiol-ene" photo- chemical and then salting reaction. The critical aggregate concentration (CAC), surface tension, minimum surface area per surfactant molecule and surface pressure at CAC were studied by both surface tension and electrical conductivity.

Utility of metagenomics next-generation sequencing in the diagnosis and treatment of severe infectious diseases in the intensive care unit.

Background: Metagenomic next-generation sequencing (mNGS) is a new method that combines high-throughput sequencing and bioinformatics analysis. However, it has not become as popular due to the limited testing equipment and high costs and lack of family awareness with not much relevant intensive care unit (ICU) research data.

Objective: To explore the clinical use and value of metagenomics next-generation sequencing (mNGS) in patients with sepsis in the ICU.

Interface Adsorption and Aggregation Behavior of Sulfonate-Based Anionic Silicone Surfactants in Aqueous Solution.

A series of sulfonate silicone surfactants possessing different hydrophobic groups were synthesized. Their adsorption and thermodynamic parameters in aqueous solutions were investigated by surface tension measurements, conductivity, transmission electron microscopy (TEM) and dynamic light scattering (DLS). These sulfonate-based anionic silicone surfactants exhibit significant surface activity and can reduce the surface tension of water to 19.

The mediating effect of achievement motivation on mindfulness and procrastination behavior of nursing students: A correlational study.

Procrastination behavior is prevalent among nursing students in China. However, little research has examined how mindfulness is associated with procrastination behavior, via achievement motivation among nursing students. The aims of this study were to investigate the relationship between procrastination behavior, mindfulness and achievement motivation, and explore the mediating effect of achievement motivation on mindfulness and procrastination behavior of nursing students.

Comprehensive guidance for antibiotic dosing in obese adults: 2022 update.

Drug dosing in obese patients continues to be challenging due to a lack of high-quality evidence to guide dosing recommendations. We first published guidance for antibiotic dosing in obese adults in 2017, in which we critically reviewed articles identified from a broad search strategy to develop dosing recommendations for 35 antimicrobials. In this updated narrative review, we searched Pubmed, Web of Science, and the Cochrane Library using Medical Subject Headings including anti-infectives, specific generic antimicrobial names, obese, pharmacokinetics, and others.

Syntheses, Properties, and Aggregation Behavior of Novel Carboxylate-Based Silicone Surfactants.

Two new anionic silicone surfactants were synthesized for the first time from dichloromethylvinylsilane or trichlorovinylsilane through hydrolysis-condensation and then thiol-ene reactions. Their structures were characterized by FT-IR, H NMR and ESI-MS. The surface tension (γ), critical aggregate concentration (CAC), surface pressure at CAC ( ) and minimum surface area per surfactant molecule ( ) were studied by surface tension and electrical conductivity, demonstrating their high surface activity at the gas/liquid interface.

Establishment of a modified Kyoto classification scoring model and its significance in the diagnosis of Helicobacter pylori current infection.

Background And Aims: We aimed to establish a modified model of the Kyoto classification score and verify its accuracy for predicting Helicobacter pylori (HP) infection during endoscopy.

Methods: Patients who underwent gastroscopy from June 2020 to March 2021 were included in this study. Atrophy, intestinal metaplasia, hypertrophy of the gastric fold, nodularity, diffuse redness, sticky mucus, spotty redness, xanthoma, map-like redness, fundic gland polyp, and regular arrangement of collecting venules (RAC) were recorded according to the Kyoto classification of gastritis.

Mediating effects of emotional intelligence on the relationship between empathy and humanistic care ability in nursing students: A cross-sectional descriptive study.

Patient-centered nursing holistic care is of utmost important to the nursing profession, and humanistic care cultivation has become a global nursing education concern. This study aimed to examine the relationship among emotional intelligence, empathy and humanistic care ability in nursing students, and to determine whether positive emotional intelligence could mediate the relationship between empathy and humanistic care ability. This study used a cross-sectional, descriptive design.

Risk factors and outcomes associated with persistent vancomycin resistant Enterococcal Bacteremia.

Background: Prior studies have identified that vancomycin resistant enterococcus (VRE) bacteremia that persists for four days or more is an independent predictor of mortality. Despite this, there is no published data to identify those patients at highest risk of developing persistent VRE bacteremia.

Methods: This was a single center, retrospective, case-control study of adult patients with a VRE bloodstream infection (BSI).

Ginger (Zingiber officinale) extract mediated green synthesis of silver nanoparticles and evaluation of their antioxidant activity and potential catalytic reduction activities with Direct Blue 15 or Direct Orange 26.

The green synthesis of silver nanoparticles (AgNPs) using a water extract of Ginger (Zingiber officinale) root by microwave irradiation and its antibacterial activities have been reported. However, AgNPs prepared from different parts of ginger root water or ethanol extract by ultrasound synthesis and their antioxidant activity and whether the biogenic could be used to catalyze the reduction of hazardous dye are unknown. This study concentrated on the facile green synthesis of AgNPs prepared from different parts (unpeeled ginger, peeled ginger, and ginger peel) of ginger root water or ethanol extract by the ultrasound-assisted method.