Cellular interactions and evolutionary origins of endosymbiotic relationships with ciliates.

As unicellular predators, ciliates engage in close associations with diverse microbes, laying the foundation for the establishment of endosymbiosis. Originally heterotrophic, ciliates demonstrate the ability to acquire phototrophy by phagocytizing unicellular algae or by sequestering algal plastids. This adaptation enables them to gain photosynthate and develop resistance to unfavorable environmental conditions.

Radiomics analysis of multiparametric MRI for preoperative prediction of microsatellite instability status in endometrial cancer: a dual-center study.

Objective: To develop and validate a multiparametric MRI-based radiomics model for prediction of microsatellite instability (MSI) status in patients with endometrial cancer (EC).

Methods: A total of 225 patients from Center I including 158 in the training cohort and 67 in the internal testing cohort, and 132 patients from Center II were included as an external validation cohort. All the patients were pathologically confirmed EC who underwent pelvic MRI before treatment.

Single-cell transcriptome analysis reveals the association between histone lactylation and cisplatin resistance in bladder cancer.

Patients with bladder cancer (BCa) frequently acquires resistance to platinum-based chemotherapy, particularly cisplatin. This study centered on the mechanism of cisplatin resistance in BCa and highlighted the pivotal role of lactylation in driving this phenomenon. Utilizing single-cell RNA sequencing, we delineated the single-cell landscape of Bca, pinpointing a distinctive subset of BCa cells that exhibit marked resistance to cisplatin with association with glycolysis metabolism.

Mechanical Properties and Microstructure of Polypropylene-Glass-Fiber-Reinforced Desert Sand Concrete.

In order to improve the performance of desert sand concrete, polypropylene fiber (PF) and glass fiber (GF) were used to prepare desert sand concrete (DSC) with different fiber and volume content, and the basic mechanical properties, such as cube compressive, tensile and flexural strengths, were tested and studied. Based on the mercury injection method (MIP) and scanning electron microscopy (SEM), the evolution of pore structure and interface structure was analyzed. The mechanism of fiber toughening was revealed at the microscopic level.

Heme oxygenase‑1 inhibits renal tubular epithelial cell pyroptosis by regulating mitochondrial function through PINK1.

Endotoxin-induced acute kidney injury (AKI) is commonly observed in clinical practice. Renal tubular epithelial cell (RTEC) pyroptosis is one of the main factors leading to the development of endotoxin-induced AKI. Mitochondrial dysfunction can lead to pyroptosis.

Glycosylated protein 4-deficient PRRSV in complementing cell line shows low virus titer.

Porcine Reproductive and Respiratory Syndrome (PRRS) threats the swine industry seriously. The spread of live vaccine virus leads to the emergence of recombinant virus, which brings biosafety problems. The replication-deficient virus as a vaccine candidate would avoid this problem.

FGFBP1 as a potential biomarker predicting bacillus Calmette-Guérin response in bladder cancer.

Accurate prediction of Bacillus Calmette-Guérin (BCG) response is essential to identify bladder cancer (BCa) patients most likely to respond sustainably, but no molecular marker predicting BCG response is available in clinical routine. Therefore, we first identified that fibroblast growth factor binding protein 1 (FGFBP1) was upregulated in failures of BCG therapy, and the increased FGFBP1 had a poor outcome for BCa patients in the E-MTAB-4321 and GSE19423 datasets. These different expression genes associated with FGFBP1 expression are mainly involved in neutrophil activation, neutrophil-mediated immunity, and tumor necrosis factor-mediated signal pathways in biological processes.

Subunit vaccine based on glycoprotein B protects pattern animal guinea pigs from tissue damage caused by infectious bovine rhinotracheitis virus.

Infectious bovine rhinotracheitis (IBR) is caused by Bovine herpesvirus type 1 (BoHV-1), which seriously threatens the global cattle industry. Only vaccination to improve immunity is the most direct and effective means to prevent IBR. Attempts are being made to use subunit vaccines, deleted or recombinant viral vaccines to reduce or eradicate IBR.

Radiomics Features at Multiparametric MRI Predict Disease-Free Survival in Patients With Locally Advanced Rectal Cancer.

Objective: To investigate the potential value of radiomics features based on preoperative multiparameter MRI in predicting disease-free survival (DFS) in patients with local advanced rectal cancer (LARC).

Methods: We identified 234 patients with LARC who underwent preoperative MRI, including T2-weighted, diffusion kurtosis imaging, and contrast enhanced T1-weighted. All patients were randomly divided into the training (n = 164) and validation (n = 70) cohorts.

Toxic effects of methomyl on mouse oocytes and its possible mechanisms.

Methomyl is a broad-spectrum carbamate insecticide that has a variety of toxic effects on humans and animals. However, there have been no studies on the toxicity of methomyl in female mammalian oocytes. This study investigated the toxic effects of environmental oestrogen methomyl exposure on mouse oocyte maturation and its possible mechanisms.

Role of Pericytes in Diabetic Angiogenesis.

In the context of diabetes mellitus, various pathological changes cause tissue ischemia and hypoxia, which can lead to the compensatory formation of neovascularization. However, disorders of the internal environment and dysfunctions of various cells contribute to the dysfunction of neovascularization. Although the problems of tissue ischemia and hypoxia have been partially solved, neovascularization also causes many negative effects.

Epidemiological Research Advances in Vascular Calcification in Diabetes.

Vascular calcification is the transformation of arterial wall mesenchymal cells, particularly smooth muscle cells (SMCs), into osteoblast phenotypes by various pathological factors. Additionally, vascular transformation mediates the abnormal deposition of calcium salts in the vascular wall, such as intimal and media calcification. Various pathological types have been described, such as calcification and valve calcification.

Diffusion kurtosis imaging-derived histogram metrics for prediction of resistance to neoadjuvant chemoradiotherapy in rectal adenocarcinoma: Preliminary findings.

Purpose: This study aimed to evaluate the potential role of diffusion kurtosis imaging (DKI)-derived parameters for assessing resistance to CRT in patients with Locally advanced rectal cancer (LARC) by using histogram analysis derived from whole-tumor volumes.

Method: 136 consecutive patients with histologically confirmed rectal adenocarcinoma who underwent MRI examination before and after chemoradiotherapy were enrolled in our retrospective study. The parameters D, K, and conventional apparent diffusion coefficient (ADC) were measured using whole-tumor volume histogram analysis.

The efficacy and safety of PD-1/PD-L1 immune checkpoint inhibitors in treating advanced urothelial cancer: a meta-analysis of clinical trials.

Survival outcomes in advanced urothelial cancer (UC) are dismal. Over the past years, immunotherapy remains an evolving treatment modality for these patients. This meta-analysis was performed to comprehensively evaluate the efficacy and safety of immune checkpoint inhibitors.

Multi-channel multi-task deep learning for predicting and mutations of non-small cell lung cancer on CT images.

Background: Predicting the mutation statuses of 2 essential pathogenic genes [epidermal growth factor receptor () and Kirsten rat sarcoma ()] in non-small cell lung cancer (NSCLC) based on CT is valuable for targeted therapy because it is a non-invasive and less costly method. Although deep learning technology has realized substantial computer vision achievements, CT imaging being used to predict gene mutations remains challenging due to small dataset limitations.

Methods: We propose a multi-channel and multi-task deep learning (MMDL) model for the simultaneous prediction of and mutation statuses based on CT images.

Advance of African swine fever virus in recent years.

African swine fever (ASF) is one of the most devastating hemorrhagic infectious diseases that affect pigs and wild suids due to the lack of a vaccine or an effective treatment. The large dsDNA genome of African swine fever virus (ASFV) contains up to 167 ORFs that are predicted to encode proteins. Since its introduction to China in 2018, this genome has aroused the enthusiasm of researchers throughout the world.

HnRNP-F promotes the proliferation of bladder cancer cells mediated by PI3K/AKT/FOXO1.

Our previous study showed that heterogeneous nuclear ribonucleoprotein F (hnRNP-F) could induce epithelial-mesenchymal transition and metastasis in bladder cancer (BC), however, the role and mechanism of hnRNP-F in mediating the proliferative ability of BC cells remain unclear. HnRNP-F promoted the proliferation of BC cells by using BC cell lines and cell counting kit-8 (CCK8), colony formation and flow cytometry assays . Furthermore, the association of hnRNP-F with the phosphoinositide 3‑kinase (PI3K)/protein kinase B (AKT) signalling pathway was confirmed by western blotting after bioinformatic analysis.

The optimization of a novel selective antagonist for human M muscarinic acetylcholine receptor.

Muscarinic acetylcholine receptors (mAChRs) comprise five distinct subtypes denoted M to M. The antagonism of M subtype could increase the release of acetylcholine from vesicles into the synaptic cleft and improve postsynaptic functions in the hippocampus via M receptor activation, displaying therapeutic potentials for Alzheimer's disease. However, drug development for M antagonists is still challenged among different receptor subtypes.

Multicenter electron-sharing σ-bonding in the AgFe(CO) complex.

The heterodinuclear silver tetracarbonyl-iron anion was generated in the gas phase and studied by joint photoelectron velocity map imaging spectroscopy and quantum chemical calculations. The AgFe(CO)4- anion is characterized to be an 18-electron complex with the silver atom covalently bonded to the anionic tetracarbonyl-iron, an isolobal analogue of the methyl radical. The bonding analyses using a range of state-of-the-art quantum chemistry methods revealed a peculiar decentralized bonding situation, where the silver atom is covalently bonded to both the iron center and the vicinal carbon atoms in the form of an electron-sharing σ bond.

Radiomics Analysis of Multiparametric MRI for the Preoperative Prediction of Lymph Node Metastasis in Cervical Cancer.

To develop and validate a radiomics predictive model based on multiparameter MR imaging features and clinical features to predict lymph node metastasis (LNM) in patients with cervical cancer. A total of 168 consecutive patients with cervical cancer from two centers were enrolled in our retrospective study. A total of 3,930 imaging features were extracted from T2-weighted (T2W), ADC, and contrast-enhanced T1-weighted (cT1W) images for each patient.

One-year change in resting heart rate and subsequent risk of hypertension in healthy Chinese adults.

Background: Changes in baseline resting heart rate (RHR) appear to predict new-onset hypertension (NOH). However, RHR is a dynamic anthropometric parameter; thus, the association between changes in RHR and NOH requires further investigation.

Methods: We studied 10 403 participants who were initially normotensive and who had at least one routine health examination at baseline and 1 year later during 2011-2016.