Exploiting long read sequencing to detect azole fungicide resistance mutations in Pyrenophora teres using unique molecular identifiers.

Resistance to fungicides is a global challenge as target proteins under selection can evolve rapidly, reducing fungicide efficacy. To manage resistance, detection technologies must be fast and flexible enough to cope with a rapidly increasing number of mutations. The most important agricultural fungicides are azoles that target the ergosterol biosynthetic enzyme sterol 14α-demethylase (CYP51).

Virulence Profiles and Genome-Wide Association Study for Isolates Collected from Australian Lentil-Growing Regions.

, the causal organism of Ascochyta blight (AB) of lentil (), has been shown to produce an avirulence effector protein that mediates AB resistance in certain lentil cultivars. The two known forms of the effector protein were identified from a biparental mapping population between isolates that have reciprocal virulence on 'PBA Hurricane XT' and 'Nipper'. The effector AlAvr1-1 was described for the PBA Hurricane XT-avirulent isolate P94-24 and AlAvr1-2 characterized in the PBA Hurricane XT-virulent isolate Kewell.

Field Pea () Germplasm Screening for Seedling Ascochyta Blight Resistance and Genome-Wide Association Studies Reveal Loci Associated with Resistance to and .

Ascochyta blight is a damaging disease that affects the stems, leaves, and pods of field pea () and impacts yield and grain quality. In Australia, field pea Ascochyta blight is primarily caused by the necrotrophic fungal species and . In this study, we screened 1,276 spp.

Phenotypic and Genotypic Diversity of Populations in Southern Australia.

Speg. is a serious foliar fungal disease of faba bean and a constraint to production worldwide. This study investigated the phenotypic and genotypic diversity of the pathogen population in southern Australia and the pathogenic variability of the population was examined on a differential set of faba bean cultivars.

The novel avirulence effector AlAvr1 from Ascochyta lentis mediates host cultivar specificity of ascochyta blight in lentil.

Ascochyta lentis is a fungal pathogen that causes ascochyta blight in the important grain legume species lentil, but little is known about the molecular mechanism of disease or host specificity. We employed a map-based cloning approach using a biparental A. lentis population to clone the gene AlAvr1-1 that encodes avirulence towards the lentil cultivar PBA Hurricane XT.

Current population structure and pathogenicity patterns of in Australia.

Ascochyta blight disease, caused by the necrotrophic fungus , is a major biotic constraint to chickpea production in Australia and worldwide. Detailed knowledge of the structure of the pathogen population and its potential to adapt to our farming practices is key to informing optimal management of the disease. This includes understanding the molecular diversity among isolates and the frequency and distribution of the isolates that have adapted to overcome host resistance across agroecologically distinct regions.

Reference genome assembly for Australian Ascochyta lentis isolate Al4.

Ascochyta lentis causes ascochyta blight in lentil (Lens culinaris Medik.) and yield loss can be as high as 50%. With careful agronomic management practices, fungicide use, and advances in breeding resistant lentil varieties, disease severity and impact to farmers have been largely controlled.

Reference Genome Assembly for Australian Isolate ArME14.

is the causal organism of ascochyta blight of chickpea and is present in chickpea crops worldwide. Here we report the release of a high-quality PacBio genome assembly for the Australian isolate ArME14. We compare the ArME14 genome assembly with an Illumina assembly for Indian isolate, ArD2.

Characterization of Growth Morphology and Pathology, and Draft Genome Sequencing of , the Causal Organism of Chocolate Spot of Faba Bean ( L.).

Chocolate spot is a major fungal disease of faba bean caused by the ascomycete fungus, . is also implicated in botrytis gray mold disease in lentils, along with . Here we have isolated and characterized two isolates from chocolate spot lesions on faba bean leaves.

Agrobacterium tumefaciens-mediated transformation and expression of GFP in Ascochyta lentis to characterize ascochyta blight disease progression in lentil.

The plant immune system is made up of a complex response network that involves several lines of defense to fight invading pathogens. Fungal plant pathogens on the other hand, have evolved a range of ways to infect their host. The interaction between Ascochyta lentis and two lentil genotypes was explored to investigate the progression of ascochyta blight (AB) in lentils.