Publications by authors named "Lina Ancheva"
Peptides From the Variable Domain of Immunoglobulin G as Biomarkers in Chronic Inflammatory Demyelinating Polyradiculoneuropathy.
Neurol Neuroimmunol Neuroinflamm
November 2023
Background And Objectives: Chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) is a clinically heterogeneous immune-mediated disease. Diagnostic biomarkers for CIDP are currently lacking. Peptides derived from the variable domain of circulating immunoglobulin G (IgG) have earlier been shown to be shared among patients with the same immunologic disease.
View Article and Find Full Text PDFArticle Synopsis
- Pre-vaccination SARS-CoV-2 infection enhances COVID-19 vaccine effectiveness, and breakthrough infections after vaccination can further improve immunity, leading to what is called 'hybrid immunity'.
- A study analyzed the complementarity determining regions (CDR) of anti-RBD antibodies from individuals with hybrid immunity and those vaccinated but not previously infected.
- Results indicate that individuals with hybrid immunity exhibit a unique CDR profile that is different from vaccinated individuals without prior infection, suggesting that both infection and vaccination contribute to more robust immune responses.
The wide diversity of proteins expressed in a cell or a tissue as a result of gene variants, RNA editing or PTMs results in several hundred thousand distinct functional proteins called proteoforms. The large-scale analysis of proteomes has been driven by bottom-up MS approaches. This allowed to identify and quantify large numbers of gene products and perform PTM profiling which yielded a significant number of biological discoveries.
View Article and Find Full Text PDFPurpose: We report an immunocapture strategy to extract proteins known to harbor driver mutations for a defined cancer type before the simultaneous assessment of their mutational status by MS. Such a method bypasses the sensitivity and selectivity issues encountered during the analysis of unfractionated complex biological samples.
Experimental Design: Fast LC separations using short nanobore columns hyphenated with a high-resolution quadrupole-orbitrap mass spectrometer have been devised to take advantage of fast MS cycle times in conjunction with sharp chromatographic peak widths to accelerate the sample analysis throughput.