Publications by authors named "Lin-kai Guo"

Extensive application of carbon quantum dots (CQDs) enlarges its concentration in sewage treatment system. The response of nitrifying sludge to CQDs after long-term exposure was investigated. Results showed that CQD concentrations of 0-100 mg/L presented positive effect to enzymes involved in nitrification, accelerating NH-N degradation and NO-N transformation.

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Article Synopsis
  • * As nCrO levels increase, Chlorella sp. produces more extracellular polymeric substances (EPS) to protect itself, but this adaptive response eventually fails, leading to severe cellular damage and metabolic disruptions.
  • * The research highlights that the toxicity of nCrO is linked to physical cell damage, increased oxidative stress, and impaired gene transcription related to essential metabolic processes at doses starting from 20 mg/L.
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The occurrence of metal oxide nanoparticles (NPs) in wastewater treatment plants (WWTPs) has raised great concerns about their adverse impacts on nitrification performance. In this study, a heterotrophic nitrifying bacterium Pseudomonas putida strain NP5 showed strong resistance against TiO and NiO NPs. Under 5-50 mg/L NP stress, cell viability was still normal, and the final nutrient removal rates, always higher than 80%, were slightly inhibited.

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Because of the massive discharge of nitrogenous wastewater, the eutrophication of a water body is becoming increasingly serious, and how to effectively remove nitrogen from this wastewater remains an urgent problem to be solved. In this study, due to disadvantages in the traditional biological nitrogen removal process, such as complex and long procedures, high energy consumption, weak impact resistance, and NO release, the nitrogen removal theory by heterotrophic nitrification was further analyzed by discussing the physiological-biochemical, heterotrophic nitrification-aerobic denitrification, and NO production characteristics of a high-efficiency heterotrophic nitrifying bacteria YL. Results show that the strain YL had an eminent heterotrophic nitrification and aerobic denitrification ability, and NH-N, NO-N, and NO-N with concentration of 100 mg·L could be completely removed during the 24-hour incubation period.

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Due to the problems of traditional biological nitrogen and phosphorus removal, including long process duration and high infrastructural and operational costs, the simultaneous nitrogen and phosphorus removal capabilities, influencing factors and kinetic characteristics were systematically studied using the heterotrophic nitrifier NP1 which possesses efficient simultaneous nitrogen and phosphorus removal ability. The results showed that strain NP1 exhibited efficient heterotrophic nitrification ability with a maximum ammonia removal rate of 99.12%.

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A novel strain NP5 with efficient heterotrophic nitrification, aerobic denitrification and phosphorus accumulation ability was isolated and identified as Pseudomonas putida strain NP5. The removed ammonium and phosphate were mainly converted into intracellular components by assimilation, and negligible nitrification intermediates and NO were accumulated during heterotrophic nitrification. In addition, the optimal conditions for nutrient removal were: succinate as carbon source, C/N 10, P/N 0.

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Objective: To study the correlation between the expression of Fcgamma receptor II b (FcgammaRII b) on B cells and rheumatoid arthritis (RA) patients of Shen deficiency syndrome (SDS).

Methods: There were 43 RA patients, including 26 of SDS and 17 of non-SDS. The expression levels of FcgammaRII b on naive B cells, memory B cells, and plasma blasts in the peripheral blood were detected by flow cytometry.

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Objective: To investigate the correlation between auto-immune antibodies and rheumatoid arthritis (RA) patients of Shen deficiency syndrome (SDS), thus providing clinical evidence for further researches on molecular biological mechanisms of RA patients of SDS.

Methods: Totally 451 RA patients were assigned to the SDS group and the non-SDS group. Their general conditions (including gender, age, duration, and age of onset), C reactive protein (CRP), erythrocyte sedimentation rate (ESR), platelet (PLT), disease activities (DAS28), auto-antibodies [rheumatoid factor (RF), anti-CCP antibodies, anti-nuclear antibody (ANA)] were compared between the two groups.

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Aim: To confirm extracts of activity from Traditional Chinese Medicine TongBiHeJi, study effect on two signaling pathways of T cells and clarify the pharmacological mechanisms of TongBiHeJi.

Methods: Concanavalin(ConA) were added successively into rats lymphocytic culture with different extracts of activity from Traditional Chinese Medicine. After 24 hours, CD71 expression rate on rat T lymphocytes activated with ConA was analyzed by flow cytometry.

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