Publications by authors named "Lin-Yi Cai"
Article Synopsis
- Osteoarthritis (OA) is the most common chronic joint disorder, characterized by various degenerative changes, but the biological mechanisms behind it remain unclear.
- Researchers analyzed gene expression data from synovial tissues to identify key biomarkers and immune cell involvement in OA, finding 18 important differentially expressed genes (DEGs).
- The study highlights specific signaling pathways related to these DEGs and suggests that the hub gene ZNF160 may play a significant role in immune cell activity, offering potential new directions for OA diagnosis and treatment.
[The Effects of NF-kappaB Signalling on the TNF-α induced Ratios of MMPs to TIMPs in Chondrocytes].
Sichuan Da Xue Xue Bao Yi Xue Ban
September 2016
Objectives: To explore the influence of the NF-κB inhibitor (bay11-7082) on tumor necrosis factor-α (TNF-α)-induced different ratios of matrix metalloproteinases (MMPs) and their tissue inhibitors (TIMPs) in chondrocytes.
Methods: Chondrocytes were isolated from the knee joint of a 1-day old mouse by trypsin digestion method. Hematoxylin-Eosin (HE) stain was used to show the morphology of isolated chondrocytes; Semi-quantitative PCR was applied to analyze the influence of bay11-7082 on gene expressions of TNF-α-induced and in chondrocytes; Zymography was used to elucidate activities of the gelatinases induced by TNF-α and/or bay11-7082.
Osteoarthritis is recognised to be an interactive pathological process involving the cartilage, subchondral bone and synovium. The signals from the synovium play an important role in cartilage metabolism, but little is known regarding the influence of the signalling from bone. Additionally, the collagenases and stromelysin-1 are involved in cartilage catabolism through mitogen-activated protein kinase (MAPK) signalling, but the role of the gelatinases has not been elucidated.
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