Fluorescence detection of intracellular pH changes in the mitochondria-associated process of mitophagy using a hemicyanine-based fluorescent probe.

Intracellular pH behaves as a vital parameter in the physiological and pathological processes. Novel small molecule probes for precise and dynamic monitoring of pH fluctuations in cellular physiological processes are still highly required. Herein, we present a hemicyanine-based probe (HcPH) detection of the pH changes during the intracellular process of mitochondria-associated autophagy.